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We narrowed to 844 results for: STI

Showing: 321 - 340 of 844 results

  1. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...the way we apprehend molecular complexes and is still a very useful tool.    Like a guitar hero (that ...SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and most used pairs to measure FRET... is difficult to address especially when you investigate unknown molecular complexes. But it is a parameter... faster measurement systems, this technique has still many good days and good “gigs” ahead...No offense...Chemiluminescence Resonance Energy Transfer    Interesting Articles About FRET: Piston DW, Kremers GJ. ...

  2. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that can drive lasting transcriptional...cofactor fused to a histone tail to recruit and stimulate the endogenous methyltransferase. The strategy...H3K4me0 on D3L, releasing the autoinhibition and stimulating the methylation of nearby CpG sites on the target...expression off. Plus, DNA methylation is a long-lasting repressive marker, allowing the team to develop...

  3. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...potential for therapeutic drug discovery as it is estimated that 75% of disease causing genes in humans have...Stainer group determined that there are actually two distinct populations of β-cells that originate in different...other model organisms. Worms have been used to investigate an array of biological systems and processes ...PubMed PMID: 9486653. Hesselson, Daniel, et al. "Distinct populations of quiescent and proliferative pancreatic...19706417. PubMed Central PMCID: PMC2736433. Howe, Kerstin, et al. "The zebrafish reference genome sequence...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...GFP-like probes by supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional...mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological Studies) Multiple uses: From...applications.  There is a whole set of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names...occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For...developed based on mIFP6. Learn more about the characteristics, performance, and applications of near-infrared...

  5. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...article is to provide enough background for you to distinguish the features of any common lab strain and determine...them in 1885. E. coli are mainly found in the intestinal tract of animals. There are many different naturally...hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated... Hte   High transformation efficiency deoR constitutive expression of genes for deoxyribose synthesis...strains; however, these tables are by no means exhaustive! For a more comprehensive list and additional...

  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...ve reduced the chance of off-target effects, you still need to do your due diligence to ensure they are...here. For some applications, this is ok, but it still must be controlled for and acknowledged as a potential...downstream characterization of, isolating 2 or 3 distinct clones to perform key experiments on is a good...principle holds true - the higher the number of distinct editing events, the lower the likelihood of an...transduction control, a different gRNA would have distinct off-target sites, eliminating its effectiveness...

  7. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...chemical input to an RSM drives expression of a distinct large serine recombinase that recognizes its own...demonstrates an example of a register that enters distinct states when treated with different combinations...designs, we have observed that certain GRSMs may still not perform as expected, perhaps due to idiosyncrasies...the same gene regulation program. We recommend testing multiple register designs for the same gene regulation...described in the literature (5, 6) and may be substituted for one another in an RSM. The predictability...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...was fascinated by CRISPR and rushed to test it. I still remember the day, March 16, 2014, that I got my ... in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...transmission of the transgene, this technique is still in need of improvement. Also when working with CRISPR... discovered in an obscure bacterium and that we still have much to learn from biology in all its forms...editing.     Haoyi Wang is a professor at the Institute of Zoology, Chinese Academy of Sciences, and he...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... transcription factors, or when the gene is constitutively-active or a dominant-negative mutant. Reducing... TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely seamless...researcher may subsequently kill off any cells that still contain the donor plasmid after excision by PBx,... technology using a site-specific nuclease to stimulate homologous recombination and Footprint-Free™ removal...and I. M. Verma, “Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from...

  10. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...Fellow in the Church and Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is...CRISPR during his postdoc 12:01 - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why .... 13:22 - 22:07 : Alex's projects at the Wyss Institute - New CRISPR tools and the importance of moving...26 - 33:08 : Plans for future work at the Wyss Institute. 33:09 - end : How Alex hopes to help Addgene ...

  11. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...far? Do you have any suggestions for this year’s teams that may be procrastinating choosing their costume...these. Addgenies have been organizing Halloween festivities for years – searching our photo archives I found... ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, ...

  12. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ... service, AAV Packaged on Request  has seen a fantastic response from the research community. Customers...minBG-jRGECO1a-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14360:...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14361: ...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...

  13. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...our in-stock service: ddPCR, purity testing, and sterility testing, plus viral genomic sequencing to confirm...webpage to get started with your browsing and requesting. If you’re curious about the service, read on...on Request AAVs are a big deal, so if you have questions, we have plenty of information available for you...

  14. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ...service and is ready to use out of the box. We estimate that providing cloning grade DNA could save scientists...Plasmids offered as cloning grade DNA As we begin testing out this service, we’re starting with 210 plasmids...molecular biology experiments such as cloning, digestions, PCR etc. and/or directly long term storage. ...service in the future using the Cloning Grade DNA Suggestion Form.  Additional resources on the Addgene blog...

  15. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...hydrogen bonds and a base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined ... ́-phosphate to the ligation). The structural/mechanistic reason for this differential is not known for...Despite this higher fidelity, Taq DNA Ligase can still detectably ligate many T:G, T:T, and A:C mismatches...In this method, substrate pools were prepared consisting of one target (template) strand and four upstream...Reaction Temperature Calculator can be used to estimate an appropriate incubation temperature when using...

  16. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...science policy career and this seemingly simple question is a moving target. There are a multitude of options...policy? I’ve intentionally started with the ‘who’ question because the science policy community is the best...start. What is science policy? THE IMPOSSIBLE QUESTION. Science policy is the work you do to creatively... I would argue that this is the most important question that you can explore. The answer will help you...interviews or essays. Note: the answer to this question may change over time and that’s perfectly normal...

  17. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    Published
    Jan. 21, 2016, 4:52 p.m.
    ...development of phage-based therapeutics. After a brief stint at the venture capital firm, Flagship Ventures, ...opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens in food products prior to Sample ... from developing therapeutics to developing a diagnostic? 36:53 - 38:00 : As an entrepreneur, is it more...Read Sample 6's Paper on Bacteriophage-based Diagnostics Additional Resources on the Addgene Blog Check...

  18. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries. We continue to ship...plasmids, analyze QC sequence, answer scientists' questions, manage our website, and continue to improve the...plasmids. The partnerships and collaborations with institutions and tech transfer offices that facilitate MTAs...Sidney St, Suite 550A, Cambridge, MA 02139 Have a question? Email: [email protected] Call: +1 (617) 225...

  19. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...to be used as research tools is particularly interesting. Although humans have known about bioluminescent...bioluminescent organisms and their characteristic radiance for some time – there are records describing them dating...ring that glows with a faint green light. To investigate this green glimmer, Shimomura collected many,... Roger Tsien resulted in much of our current mechanistic understanding of GFP’s function. His lab described...

  20. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...proteins, so you can't distinguish the different proteins from each other. To distinguish different target ...inside the cells and are thus typically used for distinguishing live from dead cells. The combination of antibody-fluorophores...fluorophore? Strong brightness makes it easier to distinguish a signal from noise; therefore, it is recommended...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...
Showing: 321 - 340 of 844 results