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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 605 results for: STO

Showing: 321 - 340 of 605 results

  1. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...closing in on the one million plasmids shared milestone in 2018, we wrote a post on the time and cost ...any of the numbers in this post is a mathematical story well beyond my ability to tell. But even from these...

  2. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation... fused to a dead Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase...

  4. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...little simplified): Find the appropriate glycerol stock within our freezers Pick the sample into the appropriate...intentionally trivial, but they're here to help tell this story. In this first example to the right (# this will...

  6. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...drug resistance, Bhang says “the [drug] resistance story panned out quickly,” and was summarized in a recent...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

  7. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical... talk about science in a language that can be understood by those who are not experts in particular scientific...

  8. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...be the focus of your poster - the data and your story. If you choose a single-color background but want...

  9. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate Harten DeMaio Studies...color-changing fluorescent timer (FT) protein to the histone H2B that allows for  observation in live cells....

  10. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...reaffirming.” Now, she’s an Associate Scientist at Bristol Myers Squibb and also writes for the Addgene blog...you’re set up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal...

  11. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase...disease has now become much like the 1980s song “Don’t Stop Believin’”; whenever you hear it (whether at a scientific...

  12. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...Eventually, these errors could cause the plasmid to stop functioning properly or be something completely ...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains...

  13. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred... Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). A cryptic plasmid...

  14. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...Additional ongoing trials The CRISPR therapies don’t stop with the list above! There are many, many more in...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog...

  15. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Resources References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity... https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, ...

  16. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... we'll provide an overview on how transcription stops, or termination. Read on to learn more! Download...basic processes for eukaryotic termination are understood and it has been noted that each eukaryotic RNA...

  17. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...genetics and anatomy, which are not yet fully understood. We attempt to deconstruct and understand this... into it! If you’re in the Victoria area, please stop by. And if not, I suspect you can find a makerspace...

  18. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ... 5) Listen to your supervisor’s viewpoint and brainstorm about mutually beneficial solutions Your supervisor...Look at the problem from their viewpoint, and brainstorm about solutions that will meet both of your needs...

  19. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...resulted from our work with teachers, and to create custom models of proteins for researchers. Modeling CRISPR...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...
Showing: 321 - 340 of 605 results