We narrowed to 600 results for: STO
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TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....2019 Society for Developmental Biology meeting in Boston. The atmosphere was electric! After attending this...(2018): 134-144. PubMed PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction ...
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Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....refers to the work put into creating a permanent store of knowledge (a schema).” In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....little simplified): Find the appropriate glycerol stock within our freezers Pick the sample into the appropriate...intentionally trivial, but they're here to help tell this story. In this first example to the right (# this will... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m....induces recombination at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks...Cre is absent. When Cre is present, it removes the STOP cassette, activating gene expression. Although Cre... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....drug resistance, Bhang says “the [drug] resistance story panned out quickly,” and was summarized in a recent...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....Eventually, these errors could cause the plasmid to stop functioning properly or be something completely ...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred... Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). A cryptic plasmid... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m....Additional ongoing trials The CRISPR therapies don’t stop with the list above! There are many, many more in...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....Resources References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity... https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, ... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical... talk about science in a language that can be understood by those who are not experts in particular scientific... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase...disease has now become much like the 1980s song “Don’t Stop Believin’”; whenever you hear it (whether at a scientific... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...be the focus of your poster - the data and your story. If you choose a single-color background but want... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....reaffirming.” Now, she’s an Associate Scientist at Bristol Myers Squibb and also writes for the Addgene blog...you’re set up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate Harten DeMaio Studies...color-changing fluorescent timer (FT) protein to the histone H2B that allows for observation in live cells.... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m.... we'll provide an overview on how transcription stops, or termination. Read on to learn more! Download...basic processes for eukaryotic termination are understood and it has been noted that each eukaryotic RNA... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....genetics and anatomy, which are not yet fully understood. We attempt to deconstruct and understand this... into it! If you’re in the Victoria area, please stop by. And if not, I suspect you can find a makerspace... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog PostPublishedJan. 21, 2015, 1:30 p.m.... 5) Listen to your supervisor’s viewpoint and brainstorm about mutually beneficial solutions Your supervisor...Look at the problem from their viewpoint, and brainstorm about solutions that will meet both of your needs... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m.... cleaving of the target strand is not as well understood but likely occurs in similar regions. This combination...up to hundreds or even thousands of base pairs, stopping either at boundaries defined by anti-CRISPR proteins... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....particles you need are not available, or the cost of custom viruses are too high for your budget. In those ...round provides the added benefit of producing a stock P1 virus, so that if you ever need to remake the... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....restriction sites allowing you to easily create customized overhangs to assemble up to 6 different fragments...PCR process (the precise mechanism isn’t well understood). Finally, TOPO cloning is another good option...