We narrowed to 351 results for: SUA

1. Antibodies 101: Introduction to Gating in Flow Cytometry

   Type

   Blog Post

   Published

   April 4, 2024, 1:15 p.m.

   ...and how you can combine two colors (stains) to visualize four populations at once.   Paul Heisig is a Research...

2. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   Published

   June 3, 2021, 1:15 p.m.

   ...connected by a flexible linker peptide that is usually 15-20 amino acids long and made up of glycine and...

3. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ...scenes footage in the last several years, you can usually see a director sitting in their chair not watching...

4. Using Phosphoserine to Study Protein Phosphorylation

   Type

   Blog Post

   Published

   June 23, 2016, 2:30 p.m.

   ...ability to isolate purified phosphorylated products usually depends on tools such as phosphospecific antibodies...

5. Evolution of Lab Techniques

   Type

   Blog Post

   Published

   June 21, 2016, 2:30 p.m.

   ...sample thereby labeling it. The label can then be visualized by autoradiography and X-ray film for radioactively...

6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ...common infections and opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy...

7. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ...reaction is typically fairly high, so there is usually no need to screen a large amount of colonies. Excluding...

8. Hot Plasmids: Spring 2025

   Type

   Blog Post

   Published

   June 3, 2025, 1:15 p.m.

   ...loose-seal, cell-attached recordings in primary visual cortex (V1) layer 2/3 (L2/3). B) Fluorescence from...

9. CRISPR 101: Off-Target Effects

   Type

   Blog Post

   Published

   Feb. 8, 2024, 2:15 p.m.

   ...pathway, a growth factor, etc.) this control is usually not very meaningful. Unfortunately, there really...

10. A Control for All Seasons

    Type

    Blog Post

    Published

    Oct. 5, 2023, 1:15 p.m.

    ...Samples are incubated with ‌secondary antibody as usual and the signal measured. If a nonspecific signal...

11. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    Published

    June 27, 2023, 1:15 p.m.

    ...it (Nagel et al., 2003). Inhibitory opsins are usually light-driven pumps that pass ions (Cl-, H+, Na+...

12. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...to describe or classify the labeled structures visually; for example, confirming that your target protein...

13. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...library of transformants for single colonies (this usually requires 10-20 plates) (see box below). This initial...

14. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...oligonucleotide, we aim to generate 50 to 100 mice and are usually successful in deriving mice carrying the intended...

15. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...not suit everyone. My career path may seem quite unusual but it has allowed me to try out working in many...

16. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ...to regulate gene expression (CRISPRi/CRISPRa), visualize specific genomic loci (CRISPR-imaging), or to ...

17. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    Published

    Feb. 25, 2020, 2:15 p.m.

    ...  Genetic Engineering Era: Pre-CRISPR  Having visualized and understood the DNA, the next milestone for...

18. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...to cell-type specific promoters; however, those usually drive weak expression of downstream genes. Strong...

19. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...differences. The original creators of this technique visualized the ligation product in a gel, but we can now...

20. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    Published

    Nov. 28, 2017, 2 p.m.

    ...experiments I’d been learning about for years were usually the products of teams: Watson, Crick, & Franklin...