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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 608 results for: Sis;

Showing: 321 - 340 of 608 results

  1. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not...determine its genotype. However, this detailed analysis is not possible with IVF, as most of the cells...

  2. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...systematically and logically is as useful for finishing your thesis as it is for helping your team reach an important...thinking, decision making, creativity, or data analysis. Conveniently, these are all skills you are already...

  3. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with half the size...promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001). PubMed PMID: 11509958...

  4. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to their abundance...considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that arise during transcription...

  5. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...using words for their field or technology in a consistent way. Just creating a lexicon was not enough. ... using the same words with the same meanings consistently, meetings will be shorter and more productive...

  6. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...technical details of the problem you're solving, but consistency is key. Some namespace collisions you may encounter...treating these pieces of data in ways that are inconsistent with how they are being treated in the lab, ...

  8. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...presence? Learn a key new job skill? Finish your thesis? Knowing your desired outcomes will help you assess...For example Dora Farkas, founder of Finish Your Thesis (who has also blogged for us) offers a free report...

  9. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...as in important physiological states such as adipogenesis and macrophage activation. Our mass spectrometry-affinity...coregulators in HeLa cells was the first comprehensive analysis of the interactome of these key regulatory molecules...

  10. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...always helpful to remember that if a PI asks for assistance in the grant application process, she has faith...deem your proposal successful. Typical proposals consist of 3 to 5 aims, with secondary milestones to meet...

  11. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...fluorescent timer emits blue light around the time of synthesis and, after a few hours, a conformational change...myeloid leukemia and saw that expression of H2B-FT consistently predicted the cell cycle speed across these ...

  13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...community involved in sampling or bioinformatics analysis or upload your data to Zooniverse. The Natural..., Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a...

  16. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...fills all those vials? Well, it used to be a lab assistant, but with increased plasmid demand it was too ...fill 400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in 45 minutes by hand...

  17. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...All destination vectors contain an ampicillin resistance cassette and an origin of replication, which ...pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...

  18. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...topics that may require additional attention during analysis. These include topics such as: locations in a ...on Addgene.org Educational Resources Sequence Analysis of a Plasmid Protocol ...

  19. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ... will be selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic... may be some low-level fluorescent protein or resistance marker expression following transfection, higher-level...

  20. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...rates, determined using sophisticated genome-wide analysis. At many of its computationally predicted off-... Hur JK, Been KW, Yoon SH, Kim JS. Genome-wide analysis reveals specificities of Cpf1 endonucleases in...
Showing: 321 - 340 of 608 results