We narrowed to 631 results for: UTY

1. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

   Type

   Blog Post

   Published

   July 27, 2017, 1:11 p.m.

   ...played enormous roles in enabling researchers to utilize and develop these tools by making their own fluorescent...Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs in a variety of research applications...

2. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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   Blog Post

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   July 21, 2020, 1:15 p.m.

   ...your DNA: Concentration (ug/ml) = A260 reading x dilution factor x 50 ug/ml This method is quick and simple...You then run samples of your DNA at different dilutions and quantify based on band intensities of your...

3. Plasmids 101: Gram Negative and Positive Bacteria

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   Blog Post

   Published

   Aug. 2, 2022, 1:15 p.m.

   ... tap water. Flood the slide with Gram's iodine solution. Rinse briefly with tap water. Add ethyl alcohol...preparing the smear, and mixing the stains and solutions used, check out these resources.   Bruckner, ...

4. DAP arrays for multiplex gene editing

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   Blog Post

   Published

   July 7, 2022, 1:15 p.m.

   ...for a higher number of MPE. Therapeutic potential  To explore the therapeutic potential of the DAP array...

5. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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   Blog Post

   Published

   Dec. 10, 2019, 1:58 p.m.

   ... T helper type 2 (Th2) cells from mice that constitutively express Cas9 and screen for genes that affect...commonly called bad cholesterol). However, size distribution and concentration of ApoB-LPs has been shown...

6. R Bodies: Membrane-Rupturing Microscopic Tools

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   Blog Post

   Published

   April 14, 2016, 2:30 p.m.

   ...we can also learn a lot from Nature’s simpler solutions to transmitting forces over long distances. For...

7. How to Lead a Great Meeting

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   Blog Post

   Published

   Feb. 9, 2016, 3:30 p.m.

   ...excited about the prospect of working toward a solution. At the end of the meeting, make sure to thank...Addgene Blog Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a ...

8. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...

9. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   Blog Post

   Published

   July 28, 2017, 1:30 p.m.

   ...genetic manipulations. You can produce some very beautiful immunofluorescent images like in this paper (2...experimental assay that works with flies, you can utilize the resources of the Drosophila community to easily...

10. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post

    Published

    May 11, 2017, 2:30 p.m.

    ...each genotype with vulnerabilities) to design a therapeutic strategy that works. But, those darn cancers ... using attributes of cancer variants such as evolutionary conservation, or predicted deleteriousness of...

11. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ... break site (Figure 2A). The single molecule resolution of digital PCR allows classification of individual...total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA from a few thousand...

12. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    Blog Post

    Published

    Jan. 21, 2025, 2:15 p.m.

    ... "do it yourself" can result in burnout and underutilized team potential. I often thought that performing...PhDs in their careers with actionable steps and execution plans.   Resources  Resources for aspiring managers...

13. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ... the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs were then verified...plasmids have been deposited with Addgene for distribution to the scientific community with the plasmid...

14. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post

    Published

    June 15, 2023, 1:15 p.m.

    ...more effectively used in a technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic...to include reagents accessible to your lab. The solution GCE provides almost endless possibilities for ...

15. Viral Vectors 101: Types of viruses

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    Blog Post

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    June 6, 2023, 1:15 p.m.

    ...gamma-retrovirus in the lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival...naturally occurring and more synthetic capsids are routinely being developed) to choose from, which can target...

16. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ...serotype also has promising therapeutic applications; it can enhance therapeutic delivery across broader ...

17. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ... Rep68 to initiate replication and a terminal resolution site that serves as the target site for Rep proteins...structure. The Rep binding element (RBE) and terminal resolution site (trs) are noted. Created with biorender....

18. Behind the scenes: Addgene’s new search engine and more

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    Blog Post

    Published

    Dec. 12, 2019, 2:22 p.m.

    ...protocols for educational resources, and labs and institutions for depositors (see below). Even if the user...suggestions provide shortcuts to matching PI labs and institutions. There are many more technical details that ...

19. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...this same antibody to any protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small...thing to do to put these out there. It’s a simple solution. It would be silly not to share it as soon as ...

20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary to improve...multiplexing afforded by PTGs will improve the utility of CRISPR/Cas9 in new avenues of research. The ...