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Addgene
Showing: 321 - 340 of 633 results

  1. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...enhance expression of your gene of interest with a variety of Cas9 activators available in lentiviral format...be used to screen for genes involved in a wide variety of cellular processes. While these are widely requested...

  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...students simply need to add water to express a variety of proteins. FD-CF reactions are also cost-effective..., but it could be widely adapted and used in a variety of settings. If you are a biology teacher at the...

  3. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...go into developing gene therapy strategies for various diseases. That’s when I thought, if it cannot be...There should be ways to adapt the game to each a variety of subjects at many different education levels....

  4. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...relax. Companies at LabCentral get access to a variety of experimental services and expertise without ... cancer. My partner for the day was Julie from Novartis who was working with cell culture and who was ...

  6. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...mechanisms to enable multiplex gene editing in a variety of cells and organisms. However, lengthy genetic...gRNAs from DAP array.7 Using DAP arrays with a variety of base editors, the Gao Lab successfully demonstrated...

  7. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for...

  8. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...efficiently. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...practices try "Why Aren't You Delegating" in the Harvard Business Review. Another excellent blog on delegation...

  9. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...bacterial cells); however, the techniques and reagents vary. Plasmid transfection into mammalian cells is fairly...performing a "kill curve" (basically growing cells in various concentrations of the selection reagent). Cells...

  10. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...University of North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the... 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services. There ...

  11. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...exhibition area, I got a sampling of the broad variety of tools in neuroscience trade. There were, of ...easily I could jump in and ask questions about the various posters I visited. If you’re interested in neuroscience...

  12. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...after the conjugation reaction is done, through a variety of methods or commercial kits.  However, antibodies...create a small, usable panel.  First, fluorophores vary in brightness. You don’t want a super-bright fluorophore...

  13. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... versus other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...

  14. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ... confirm expression in target cells. While the various AAV serotypes can be used to achieve different ...supply of transgenic reporter lines that supply a variety of GFP expression patterns, thus allowing you to...

  16. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

  17. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ..., we are here to help with resources from AAV variables that matter to adenovirus production tips. For...DOI:10.3390/v13030388 Coffin J.M., Hughes S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold...

  18. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...protein modifications of interest or any of a huge variety of ncAAs to study the relationship between structure... our blog – we’ll be posting more posts on the various protocols and helping to spread the word as new...

  19. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...

  20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...plasmids can direct the production of virus with various tropisms.  Packaging using helper-free packaging... virus is produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables ...
Showing: 321 - 340 of 633 results