We narrowed to 642 results for: Vars;
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TypeBlog PostPublishedMay 11, 2023, 1:15 p.m.... for a variety of reasons (Baker, Nature). Tagging your protein allows you access to a variety of antibodies...
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Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing... Various Addgenies... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) are fused...Localization of antibody-combining sites within the variable portions of heavy and light chains. Proceedings... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....management team). Sreyan is a research associate at Harvard Medical School and runs everything science (CSO... get through to a number of experts, including Harvard/MIT faculty, venture capital and angel investors... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m.... start somewhere. I knew if I started with the Harvard Business Review or some Chartered Institute of ...further education may allow you to transition into various realms of business. Thank you to our guest blogger... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....resource allows the undecided to learn about a variety of science professions based on their skills and...future regulations impact your work? What design variables need consideration? Enrich your knowledge and ... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....students simply need to add water to express a variety of proteins. FD-CF reactions are also cost-effective..., but it could be widely adapted and used in a variety of settings. If you are a biology teacher at the... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....go into developing gene therapy strategies for various diseases. That’s when I thought, if it cannot be...There should be ways to adapt the game to each a variety of subjects at many different education levels.... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....relax. Companies at LabCentral get access to a variety of experimental services and expertise without ... cancer. My partner for the day was Julie from Novartis who was working with cell culture and who was ... -
DAP arrays for multiplex gene editing
TypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....mechanisms to enable multiplex gene editing in a variety of cells and organisms. However, lengthy genetic...gRNAs from DAP array.7 Using DAP arrays with a variety of base editors, the Gao Lab successfully demonstrated... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....enhance expression of your gene of interest with a variety of Cas9 activators available in lentiviral format...be used to screen for genes involved in a wide variety of cellular processes. While these are widely requested... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that were... Various Addgenies... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....Facility Isotype IgG2a Differences: Distinct variable domains that recognize different epitopes on GFP...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....efficiently. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...practices try "Why Aren't You Delegating" in the Harvard Business Review. Another excellent blog on delegation... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....bacterial cells); however, the techniques and reagents vary. Plasmid transfection into mammalian cells is fairly...performing a "kill curve" (basically growing cells in various concentrations of the selection reagent). Cells... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m....exhibition area, I got a sampling of the broad variety of tools in neuroscience trade. There were, of ...easily I could jump in and ask questions about the various posters I visited. If you’re interested in neuroscience... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....after the conjugation reaction is done, through a variety of methods or commercial kits. However, antibodies...create a small, usable panel. First, fluorophores vary in brightness. You don’t want a super-bright fluorophore... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m.... versus other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....University of North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the... 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services. There ... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...