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...and New Faculty" downloadable book. Skip to Chapter 3 for tips on how to develop your role as a lab leader...start their own academic research laboratory. How does one even go about starting a lab? Obviously, funding...always helps. But how does one build a strong collaborative research team motivated by similar visions and...booklet. What has been the most surprising thing about becoming a PI? Tom Ellis has a one word answer: ... has to deal with. My inbox currently stands at about 65,000 messages and dealing with email can be half...the first couple of hires to build a strong, collaborative team. And take advantage of the many resources...Medical School) for taking the time to speak with us about adding new scientists to their labs. Further Reading...

...ready for the live audiences in your future.     3. Start something In almost every career talk I present...mentoring group (see above). However, now that we are all so good with video calls, how about trying your hand...blog or podcast, initiated and maintained a collaborative project, served on the board of a professional...appointments with people all over the world. Think about contacting alumni of your lab or university or use...suggest you speak with. See this post that’s all about informational interviews, for more details on how...progress on both building relationships and thinking about future career options. 5. Write a grant - even if...

...and (3) development of tools for data analysis/visualization using R/Shiny. She is passionate about open...section-specific comments, I re-read what I wrote about the paper after my initial read, adjust my language...wish for The last thing that I wanted to mention about the peer-review, is that as a peer-reviewer, you...each peer-review, I try to mention what I liked about this paper and recognize the hard work that the ...review article Resources on Addgene.org Learn about depositing plasmids pre-publication Find molecular...

...Brandon Sanderson "The Mistborn series is the first 3 books of a larger epic of fantasy novels. Set in a...resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities at Addgene...scientists bring biases to research and don't collaborate with others to bring diverse perspectives. Everyone...Science is a terrifying and equally good wake up call about the science of race."     - Joanne Kamens, Executive...easy reads, some are hard. Some I’ll never think about again, some I loved so much I’ll force them on everyone...

... of the Brunello library are also shown in Figure 3. I hope you’ll agree with me that they look very similar... and we thought it would be a good time to talk about the amplification and verification processes we ... DNA. These reactions should allow us to obtain about 100 to 1,000 bacterial colonies per individual plasmid...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...you have any tips, input, or stories of interest about library use, let us know in the comments! Additional...NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org...

...et al., 2014) (Addgene #104106 - #104108) (Figure 3) that can be introduced using in utero electroporation...Additional resources on the Addgene blog Read about Brainbow Learn about using AAVs for neuronal tracing Visit...page Additional resources on Addgene.org Learn about Addgene's viral service See our fluorescent protein...

...stressed or aging cell populations. Figure 3: Micrographs of wild type (top panels) and cells with... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...non-overlapping cells. Until now, tools for doing this were labor-intensive and required using manipulations such ...

...thousands of loops. These loops not only provide a 3-D structure for packing genomic DNA into nuclei, but... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...neurons. Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven...

...Lebbink RJ, Rehmann H, Geijsen N. Cell. 2015 Apr 23;161(3):674-690. doi:10.1016/j.cell.2015.03.028. PubMed. ... a protocol has been established) may be less laborious than viral purification, and it does not raise...

.../ I203R) (2) and PA-mRFP1(S146H / I161V / I197H) (3) were derived from DsRed and mRFP, respectively. When...PubMed PMID:19169259. PubMed Central PMCID:PMC2901231. 3.Verkhusha, Vladislav V., and Alexander Sorkin. "Conversion...emitted, making it easier to capture a clear image above background. The majority of PA-FPs come in 2 flavours...Additional Resources on the Addgene Blog Read More about Fluorescent Protein Imaging Choosing Your Fluorescent...

... motivated reasoning. Psychological bulletin, 108(3), 480. More resources on the Addgene blog Summer SciComm...discussions about the pros and cons of different fabrics while we worked. We also had discussions about the difference...grad student. I chose to focus on misinformation about sexual and reproductive health, as the stigma around... misinformation can flourish. I’ve never forgot about all the anxious, whispered questions from friends...the study design or by their preconceived ideas about the controversial medical issues. Participants ...none of the participants had preconceived opinions about “adeolitis.” The participants in my study were ...make reusable cloth menstrual pads while we talked about the environmental impact of disposable products....

...because it required only about 15 minutes of my hands on time and a total of about 3 hours. It was robust,...Addgene lab. In this post, I’ll tell you a little bit about what I learned from a New England Biolabs (NEB) ...this, but thought we could learn a little bit more about high throughput DNA prepping by trying out NEB’s... new teaching space at Lab Central to learn all about it. LabCentral is a lab and office space shared ...without breaking their bank accounts. When I heard about how LabCentral aims to enable emerging biotech companies...different biotech companies to interact and learn more about one another. They also provided both breakfast, ...go to a workshop because it will help you think about the most useful pieces of information from the workshop...

... scientists have certainly not lost site of that. 3. Stem cell science is hard, and expensive Dr. Yamanaka...the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent role in the ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years... a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular...proof that these researchers are thriving in a collaborative, rather than a combative, field. Of course they...

...the filters on the right to find single, dual, and 3+ cutters. You can also sort enzymes by clicking on...see the new map in action). Read on to learn more about the improvements we’ve implemented and how they’...more functional display, you can start thinking about your next cloning experiment early on. Beyond these... to to download the static image file (PNG, see above) for your own reference or to paste directly into...identified a plasmid that you want to learn more about, updates to our sequence analyzer will allow you...the features shown in the static maps discussed above, but also have interactive functionalities. You ...enzyme, primer, or ORF to learn more information about it. For example, if you hover over the ampicillin...

...an updated protocol), send an email instead. The 3 A's - Agendas, Action items, and Accountability Agendas...each of your meeting types (see above). We’ve provided some tips above for what works well at Addgene,...opposite effect. A great meeting should produce collaboration - a sense of dialogue and community among participants...meeting to discuss something difficult, be excited about the prospect of working toward a solution. At the...sections for each person to add a bullet or two about what they are working on. Building this into the...purpose of the meeting (see “defining the scope” above) or if you can cut it down to thirty minutes. ...some tips. Before long, you will be running collaborative, informative, energizing meetings all your own...

...produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based on ...sponsored the capital construction of our new BL2+ laboratory and the infrastructure capabilities to produce...our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently...helping thousands of sharing scientists from laboratories all over the world. With this reach and exposure...In early 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services....provides viral vector technical service (details about virus production protocols and quality control can...material. The Outreach Team is getting the word out about this new resource and providing fantastically useful...

...great tool for someone starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do ...saved and cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook...enzyme, or name to discover all the information about that restriction enzyme or share it via email with... this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS... new researchers or students beginning to learn about biochemistry. The least this app will do is allow...

...specifically expressing Cre in the hippocampus (Fig. 3). The Cre-dependent AAV would access the neurons (... Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service Resources...We often think about the brain’s function in terms of its regions. But equally important is the way the...head bone’s connected to the neck bone,” but with about a hundred billion neurons and six hundred trillion...

...recombination. In most recombineering strategies, only 3-4 phage proteins are needed and these can be provided...from previously discussed methods. As described above, you design two primers, each containing the desired...suited for insertions and deletions. The figure above shows guidelines for primer design; a desired insertion...mutation-containing colonies/total colonies) is about 84% for each of the three methods. However, REPLACR-mutagenesis... Additional Resources on the Addgene Blog Read about Other Plasmid Cloning Techniques like SLIC and Golden...

...page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information Please enter ...deposits of 10 or more plasmids.     Figure 3: If submitting plasmid(s) online (left), choose to...