...Lebbink RJ, Rehmann H, Geijsen N. Cell. 2015 Apr 23;161(3):674-690. doi:10.1016/j.cell.2015.03.028. PubMed. ... a protocol has been established) may be less laborious than viral purification, and it does not raise...

.../ I203R) (2) and PA-mRFP1(S146H / I161V / I197H) (3) were derived from DsRed and mRFP, respectively. When...PubMed PMID:19169259. PubMed Central PMCID:PMC2901231. 3.Verkhusha, Vladislav V., and Alexander Sorkin. "Conversion...emitted, making it easier to capture a clear image above background. The majority of PA-FPs come in 2 flavours...Additional Resources on the Addgene Blog Read More about Fluorescent Protein Imaging Choosing Your Fluorescent...

...because it required only about 15 minutes of my hands on time and a total of about 3 hours. It was robust,...Addgene lab. In this post, I’ll tell you a little bit about what I learned from a New England Biolabs (NEB) ...this, but thought we could learn a little bit more about high throughput DNA prepping by trying out NEB’s... new teaching space at Lab Central to learn all about it. LabCentral is a lab and office space shared ...without breaking their bank accounts. When I heard about how LabCentral aims to enable emerging biotech companies...different biotech companies to interact and learn more about one another. They also provided both breakfast, ...go to a workshop because it will help you think about the most useful pieces of information from the workshop...

... motivated reasoning. Psychological bulletin, 108(3), 480. More resources on the Addgene blog Summer SciComm...discussions about the pros and cons of different fabrics while we worked. We also had discussions about the difference...grad student. I chose to focus on misinformation about sexual and reproductive health, as the stigma around... misinformation can flourish. I’ve never forgot about all the anxious, whispered questions from friends...the study design or by their preconceived ideas about the controversial medical issues. Participants ...none of the participants had preconceived opinions about “adeolitis.” The participants in my study were ...make reusable cloth menstrual pads while we talked about the environmental impact of disposable products....

... scientists have certainly not lost site of that. 3. Stem cell science is hard, and expensive Dr. Yamanaka...the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent role in the ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years... a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular...proof that these researchers are thriving in a collaborative, rather than a combative, field. Of course they...

...the filters on the right to find single, dual, and 3+ cutters. You can also sort enzymes by clicking on...see the new map in action). Read on to learn more about the improvements we’ve implemented and how they’...more functional display, you can start thinking about your next cloning experiment early on. Beyond these... to to download the static image file (PNG, see above) for your own reference or to paste directly into...identified a plasmid that you want to learn more about, updates to our sequence analyzer will allow you...the features shown in the static maps discussed above, but also have interactive functionalities. You ...enzyme, primer, or ORF to learn more information about it. For example, if you hover over the ampicillin...

...an updated protocol), send an email instead. The 3 A's - Agendas, Action items, and Accountability Agendas...each of your meeting types (see above). We’ve provided some tips above for what works well at Addgene,...opposite effect. A great meeting should produce collaboration - a sense of dialogue and community among participants...meeting to discuss something difficult, be excited about the prospect of working toward a solution. At the...sections for each person to add a bullet or two about what they are working on. Building this into the...purpose of the meeting (see “defining the scope” above) or if you can cut it down to thirty minutes. ...some tips. Before long, you will be running collaborative, informative, energizing meetings all your own...

...produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based on ...sponsored the capital construction of our new BL2+ laboratory and the infrastructure capabilities to produce...our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently...helping thousands of sharing scientists from laboratories all over the world. With this reach and exposure...In early 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services....provides viral vector technical service (details about virus production protocols and quality control can...material. The Outreach Team is getting the word out about this new resource and providing fantastically useful...

...great tool for someone starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do ...saved and cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook...enzyme, or name to discover all the information about that restriction enzyme or share it via email with... this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS... new researchers or students beginning to learn about biochemistry. The least this app will do is allow...

...specifically expressing Cre in the hippocampus (Fig. 3). The Cre-dependent AAV would access the neurons (... Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service Resources...We often think about the brain’s function in terms of its regions. But equally important is the way the...head bone’s connected to the neck bone,” but with about a hundred billion neurons and six hundred trillion...

...recombination. In most recombineering strategies, only 3-4 phage proteins are needed and these can be provided...from previously discussed methods. As described above, you design two primers, each containing the desired...suited for insertions and deletions. The figure above shows guidelines for primer design; a desired insertion...mutation-containing colonies/total colonies) is about 84% for each of the three methods. However, REPLACR-mutagenesis... Additional Resources on the Addgene Blog Read about Other Plasmid Cloning Techniques like SLIC and Golden...

... Obligate aerobe Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose...pombe have dominated the research scene. But what about the other yeasts – in the biomedical world, are ...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii...

... epitopes that will likely bring about unwanted immunogenicity. The 3-D models can be applied to corroborate...limitations. Using methods together can inform decisions about in vivo application and clinical testing. This would...to drugs: adverse reaction terminology. WHO Collaborating Centre for International Drug Monitoring, Uppsala...

...Cepko 83898 AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 Gordon Fishell   Chemogenetics Chemogenetics offers...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

... lab E. coli K12 strains such as DH5alpha contain 3 methylases that recognize and methylate different ...questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction ...be methylated. Using the same example plasmid as above (which has the XbaI site blocked by methylation)...

...development since its inaugural year in 2012. That’s just 3 to 4 years ago. It is a bubble of innovation (a piñata...PMID: 23873081. PubMed Central PMCID: PMC3969858. 3. Doench, John G., et al. "Rational design of highly...databases mentioned above or, even better, first-hand knowledge from laboratories employing CRISPR on ... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...targetable NGG PAM sites whereas the human genome has about 300 million. With such a sparsely targetable genome...worse by an ever increasing marketplace. Since 2013 about 11 tools have been added every year.   Breaking ...databases are still young and will require a collaborative effort from the community in order to succeed...

...mutations was surprising,” says Balazs.   Figure 3: Neutralization outcomes of convalescent sera with...Browse all COVID-19 related blog posts Learn more about pseudotyping Resources on Addgene.org Find plasmids...

...cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et al 2022.   Find CRISPR Csm plasmids... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...

...). Cells should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how...

...guide RNA, (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite...sites with A's, producing C → A edits. Figure 3: Mechanism of cytosine transversion editors. Red arrows...still isn’t over! Keep an eye out for our next post about base editing, where we’ll tackle transversion editors...CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime Editing...