Antibodies 101: Beyond Surface Labeling
Type
Blog Post
Published
June 27, 2024, 1:15 p.m.
...would label everything but the CD8+ T cells (Figure 2). Even better, we can use the same fluorophore for...gating on the negative population.
Figure 2: Dump gating labels all unwanted cell lineages with...labeling can be subdivided into two categories, depending on the location of the targeted markers. Some commercially...proteins, such as receptors, or post-translational modifications on those proteins, like glycosylation patterns...Depending on the research question and downstream application, one or several of those techniques can be used...your flow analysis. However, certain downstream applications, like an in vivo adoptive transfer of those ...labeling
Oftentimes, a marker of interest is not located on the cell membrane but inside the cell. Due to...