We narrowed to 363 results for: nsf

1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ...explains it, her work has always been focused on gene transfer, from transgenes to plants, chloroplast to the...

2. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   Published

   Sept. 6, 2022, 1:15 p.m.

   ...S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector dose–response...

3. DNA Purification Without a Kit

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   Blog Post

   Published

   Feb. 11, 2020, 2:15 p.m.

   ...could interfere with interpreting the results of transfection assays. Go forth and perform your DNA extractions...

4. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ... lipoic acid ligase, biotin ligase, phosphopantetheinyltransferase) – These enzymes catalyze the covalent...

5. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ...enhancers and genome math come in. They help us to transform raw genomic coordinates into meaningful experiments...

6. A Career in Grants: How To Become a Grant Professional

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   Blog Post

   Published

   Aug. 20, 2015, 2:30 p.m.

   ...management, and program evaluation are highly transferable. Use your experience and your passion(s) to ...

7. Plasmids 101: Protein tags

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   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ...peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant proteins...

8. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   Published

   Dec. 2, 2014, 7:23 p.m.

   ...of new technologies may be very effective in transforming conventional research. So, I hope my younger...

9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   Published

   May 15, 2014, 3:33 p.m.

   ...earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions...

10. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ... for presenting a science talk Check out our transferable skills guide Read all of our career blog posts...

11. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ...Charles G. Kurland. "Growth rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal...

12. To Codon Optimize or Not: That is the Question

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    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ...important? Translation and protein synthesis relies on transfer RNAs (tRNAs). tRNAs bind to and deliver amino ...

13. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...structures within cells. HeLa cells were transfected with plasmids encoding FPs tagged with the plasma...

14. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...automating the bioinformatics steps needed to transform the raw sequencing data into a complete plasmid...

15. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...target. In addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence...

16. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...protein of interest, such as a sample transiently transfected or stably infected with the protein of interest...

17. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...Protocols Agarose Gel Electrophoresis Bacterial Transformation How to Design a Primer Creating Bacterial Glycerol...

18. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ... The benefit is that these skills are highly transferable and will help you enter other roles in project...

19. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    Published

    Aug. 5, 2014, 6:51 p.m.

    ...responsible for storage.  If datasets are too large to transfer to a journal repository, they are likely too large...

20. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate ...