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We narrowed to 359 results for: plasmids 101

Showing: 321 - 340 of 359 results

  1. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...come to the right place. Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC....2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...doi: 10.1016/j.cell.2014.07.051. PMID: 25259918 Muscle over mind. Moon HY, van Praag H. doi: 0.1016/j.cmet...

  2. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that...differentiation. Cell. 2023. 186 (1) 209-229. doi: 10.1016/j.cell.2022.11.026 Joung J. et al., Genome-scale...

  3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...Controlled Release 298:128–141 . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, Mou H, Li S, Li Y...

  4. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...generation of mutant cell lines. Further, for each plasmid in the kit, the transcript most commonly found ...initiative to curate our collection of cancer-related plasmids such that each is linked to a known major pathway...pathway. Now, using our new Cancer Research Plasmids and Resources page, investigators may select a pathway...investigator to a list of all available Addgene plasmids he or she can use to study it (as seen in the ...Visit our Cancer Collection Pages Deposit Your Plasmids for Studying Cancer Browse Our Other Collections...Cancer cell 25.3 (2014): 272-281. PubMed PMID: 24651010. 3. Ledford, Heidi. "The ras renaissance." Nature...

  5. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...Table 1). The four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME...  The X-CHIME deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations...first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral Fellow...019-09656-2 More resources on the Addgene blog CRISPR101: Validating Your Genome Edit How to Design Your...

  6. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...Addgene.org Find plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent...acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau...109 cells/ml, which is within the range of  the ~1011 commensal bacteria per gram of stool for humans....

  7. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene activation...bacterial immune system cleaves bacteriophage and plasmid DNA. Nature 468:67–71 . https://doi.org/10.1038...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids ...2017) RNA editing with CRISPR-Cas13. Science 358:1019–1027 . https://doi.org/10.1126/science.aaq0180  ... Target DNA. Cell 156:935–949. https://doi.org/10.1016/j.cell.2014.02.001  Ran FA, Cong L, Yan WX, Scott...AAV Vectors. Cell 181:136–150 . https://doi.org/10.1016/j.cell.2020.03.023 Additional Resources on the ...

  8. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. PubMed PMID: 26422227 Find plasmids from this publication... is well suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche...Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver...Biotechnology (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. 3. Zetsche ...

  9. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers...more about SHERLOCK and DETECTR Learn more about plasmids available for COVID-19 research Resources on ...in our CRISPR guide Browse our entire COVID-19 plasmid collection ...and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton JP, Deng X, Yu G, Fasching...Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch, J. N., Valois, E., Solley...Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang, F., Abudayyeh, O. O. &...

  10. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids Visit the Fluorescent Protein Guide...Frank Hayward et al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore...Lab is eager to hear from you. Find the MagLOV plasmid here!   References and resources References Duret...Biophysical Journal, 116(3), 454–468. https://doi.org/10.1016/j.bpj.2019.01.003.  Frank Hayward, R., Rai, A.,...

  11. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...validated editing reagents can be found on our plasmid pages. We encourage any labs performing genome ...CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our List of gRNA Design...ribonucleoproteins." Genome research 24.6 (2014): 1012-1019. PubMed PMID: 24696461. PubMed Central PMCID: ...

  12. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in mind...tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which expresses the activation helper protein. ...Nureki O. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Genome-scale...Weissman JS. Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9.     ...

  13. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ... [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for...Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab ... PubMed PMID: 24291815. PubMed Central PMCID: PMC4101921. 19. Dias, J., et al., Generation of red blood... PubMed PMID: 21434814. PubMed Central PMCID: PMC3161101. 20. Chang, C.J., et al., Production of embryonic...

  14. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...the upstream recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount...Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology Reference...proportions of cells. bioRxiv 788679; https://doi.org/10.1101/788679 Lin R, Wang R, Yuan J, Feng Q, Zhou Y, Zeng...

  15. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...Introduction to PCR.) But if you're ready to go beyond the 101 and take your understanding to the next level, read..., Pages 19-28, ISSN 2214-7535, https://doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes V; Garson JA...Bioinformatics, Volume 27, Issue 7, 1 April 2011, Pages 1019–1020, https://doi.org/10.1093/bioinformatics/btr065Freeman... Pages 761-774, SSN 0167-7799, https://doi.org/10.1016/j.tibtech.2018.12.002.Teo IA; Choi JW;  Morlese...Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

  17. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ... allows us to provide scientists with complete plasmid sequences. After launching our viral service in... the packaging cell line, helper and packaging plasmids. We decided to use this to our advantage by designing...Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001 Additional resources on the...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...results in as little as an hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection...sensitivity from patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR...Systems. Molecular Cell 60:385–397 . https://doi.org/10.1016/j.molcel.2015.10.008 Zetsche B, Gootenberg JS, ...CRISPR-Cas System. Cell 163:759–771 . https://doi.org/10.1016/j.cell.2015.09.038 Additional resources on the ...

  19. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... our CRISPR guide Find CRISPR plasmids for your research Browse plasmids for base editing References ...Journal of Hygiene 27:113–159 . https://doi.org/10.1017/s0022172400031879 Hershey AD, Chase M (1952) INDEPENDENT...Sciences de la Vie 323:1053–1060 . https://doi.org/10.1016/s0764-4469(00)01250-6 Ran FA, Hsu PD, Wright J,...

  20. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors...to ~1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011 - 1x1012... 1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage...
Showing: 321 - 340 of 359 results