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We narrowed to 352 results for: plasmids 101

Showing: 321 - 340 of 352 results

  1. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. PubMed PMID: 26422227 Find plasmids from this publication... is well suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche...Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver...Biotechnology (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. 3. Zetsche ...

  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers...more about SHERLOCK and DETECTR Learn more about plasmids available for COVID-19 research Resources on ...in our CRISPR guide Browse our entire COVID-19 plasmid collection ...and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton JP, Deng X, Yu G, Fasching...Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch, J. N., Valois, E., Solley...Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang, F., Abudayyeh, O. O. &...

  3. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids Visit the Fluorescent Protein Guide...Frank Hayward et al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore...Lab is eager to hear from you. Find the MagLOV plasmid here!   References and resources References Duret...Biophysical Journal, 116(3), 454–468. https://doi.org/10.1016/j.bpj.2019.01.003.  Frank Hayward, R., Rai, A.,...

  4. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...validated editing reagents can be found on our plasmid pages. We encourage any labs performing genome ...CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our List of gRNA Design...ribonucleoproteins." Genome research 24.6 (2014): 1012-1019. PubMed PMID: 24696461. PubMed Central PMCID: ...

  5. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in mind...tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which expresses the activation helper protein. ...Nureki O. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Genome-scale...Weissman JS. Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9.     ...

  6. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ... [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for...Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab ... PubMed PMID: 24291815. PubMed Central PMCID: PMC4101921. 19. Dias, J., et al., Generation of red blood... PubMed PMID: 21434814. PubMed Central PMCID: PMC3161101. 20. Chang, C.J., et al., Production of embryonic...

  7. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...the upstream recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount...Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology Reference...proportions of cells. bioRxiv 788679; https://doi.org/10.1101/788679 Lin R, Wang R, Yuan J, Feng Q, Zhou Y, Zeng...

  8. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...Introduction to PCR.) But if you're ready to go beyond the 101 and take your understanding to the next level, read..., Pages 19-28, ISSN 2214-7535, https://doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes V; Garson JA...Bioinformatics, Volume 27, Issue 7, 1 April 2011, Pages 1019–1020, https://doi.org/10.1093/bioinformatics/btr065Freeman... Pages 761-774, SSN 0167-7799, https://doi.org/10.1016/j.tibtech.2018.12.002.Teo IA; Choi JW;  Morlese...Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

  9. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ... allows us to provide scientists with complete plasmid sequences. After launching our viral service in... the packaging cell line, helper and packaging plasmids. We decided to use this to our advantage by designing...Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001 Additional resources on the...

  11. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...results in as little as an hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection...sensitivity from patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR...Systems. Molecular Cell 60:385–397 . https://doi.org/10.1016/j.molcel.2015.10.008 Zetsche B, Gootenberg JS, ...CRISPR-Cas System. Cell 163:759–771 . https://doi.org/10.1016/j.cell.2015.09.038 Additional resources on the ...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... our CRISPR guide Find CRISPR plasmids for your research Browse plasmids for base editing References ...Journal of Hygiene 27:113–159 . https://doi.org/10.1017/s0022172400031879 Hershey AD, Chase M (1952) INDEPENDENT...Sciences de la Vie 323:1053–1060 . https://doi.org/10.1016/s0764-4469(00)01250-6 Ran FA, Hsu PD, Wright J,...

  13. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors...to ~1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011 - 1x1012... 1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage...

  14. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...Chemical Biology, 26(4), 584-592.e6. https://doi.org/10.1016/j.chembiol.2019.01.003 Gallagher, S. S., Sable,...Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016/j.chembiol.2008.01.007 Griffin, B. A., Adams, S...

  15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    .... elegans. These modular toolkits build single plasmid targeting vectors that encode the gRNA transcript... C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to...Precision Genome Editing. BioRxiv. https://doi.org/10.1101/354480 Ghanta KS, Mello CC (2020) Melting dsDNA...elegans. Methods 121–122:86–93 . https://doi.org/10.1016/j.ymeth.2017.03.023 Paix A, Wang Y, Smith HE, Lee...

  16. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...get right. Here at Addgene, we use a linearized plasmid standard. However, even when the standard is made... could end up with a titer of 4 x 1012 genome copies/mL or 8 x 1012 genome copies/mL and both results ... Since AAV titers tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before...

  17. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ... Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors... Autophagy Research. Cell,140(3), 313-326. doi:10.1016/j.cell.2010.01.028. PubMed PMID: 20144757. PubMed... and Non-Mammalian Systems, Part A, 33-42. doi:10.1016/s0076-6879(08)03203-5. PubMed PMID: 19185711. Additional...

  18. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...on the Addgene blog Read blog posts related to plasmid protocols Find protocols for purifying DNA without...BBA) - Enzymology 341:484–488. https://doi.org/10.1016/0005-2744(74)90240-x Chirgwin JM, Przybyla AE, ...Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006...

  20. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...23;161(3):674-690. doi:10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested...
Showing: 321 - 340 of 352 results