Skip to main content
Addgene
Showing: 321 - 340 of 597 results

  2. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ... blog. CRISPR-based genome engineering has revolutionized the gene editing field by making experimental...considerably easier, faster, and more efficient than previous methods. Still, generating reliable results from...

  4. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... you will not be a graduate student or postdoc forever, and you need to be thinking about what comes next... development center, if one exists. Get online! Review company and institution career pages. Make yourself...

  5. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...takes on cases requiring in-depth analysis, data review, in-house testing etc. Technical support roles ...too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting protocols,...

  6. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate...gRNAs into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9...

  7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...require a PAM, and RNA targeting is potentially reversible since there is no genomic edit. Some Cas13 enzymes...KW, Yoon S, Kim J-S (2016) Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells...

  8. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...Bacterial Plasmids. Microbiology and Molecular Biology Reviews 62(2): 434–464. Pubmed PMID: 9618448 Diaz R, Rech...RP (1987) Plasmid incompatibility. Microbiology Reviews. 1987;51(4):381–395. Pubmed PMID: 3325793 Schumacher...

  9. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...the questions.  After the code is written, it is reviewed by at least one other developer. This is another...point of collaboration for us. I enjoy doing code reviews because it’s like a little peek into the mind of...

  11. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David...conversion by engineered glycosylase. National Science Review, 10(8), nwad143. https://doi.org/10.1093/nsr/nwad143...

  12. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. ...involves insertion of landing sites via Cas9-directed reverse transcriptases, followed bylanding site recognition...

  13. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...company you currently use. You can also find many previously validated gRNAs at Addgene. In vitro screening... and stuff ups). This is a huge improvement on previous mouse work, which could take 8 months or more....

  14. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...subcultured at densities that are low enough to prevent confluency but high enough to promote cell growth...Incidence, sources, effects, detection, elimination, prevention.” Cytotechnology 39.2 (2002). 75-90. Pubmed PMID...

  15. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...seizures or some other unwanted phenomenon or it could reveal something fascinating. Behavior is based primarily...behavioral test might be contextual fear conditioning (reviewed in [4]). Contextual fear conditioning is a behavioral...

  16. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...CRISPR editing CRISPR, of course, has started a revolution in gene editing. However, while CRISPR is remarkably...CRISPR-based approaches is described in several recent reviews (Howes and Schofield, 2015; Moser and Hirsch, 2016...

  18. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second crossover... in 5 days. Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab...

  20. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...antibody dilution — both factors we’ve covered in our previous blog posts (1, 2) on western blotting. If the ...confidence in the accuracy of western blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/...
Showing: 321 - 340 of 597 results