We narrowed to 430 results for: sequence

1. Easing the Protein Purification Process with pCri

   Type

   Blog Post

   Published

   June 19, 2015, 3:08 p.m.

   ...subtilis, contains the MISTIC (membrane-integrating sequence for translation of integral-membrane protein constructs...

2. Addgene is Expanding Our Viral Vector Service!

   Type

   Blog Post

   Published

   Oct. 15, 2024, 1:15 p.m.

   ...testing, sterility testing, and viral genomic sequencing to confirm the identity of the packaged DNA and...

3. New CRISPR Web Resources and #12DaysofCRISPR Recap

   Type

   Blog Post

   Published

   Dec. 12, 2017, 3:13 p.m.

   ... CRISPR 101: Validating Your Genomic Edit and Sequencing Options for CRISPR Genotyping Day 5: Delivery...

4. SunTag and Fluorescent Imaging

   Type

   Blog Post

   Published

   March 28, 2017, 2:30 p.m.

   ...expression, Tanenbaum et al. modified the GCN4 sequence to increase its alpha-helical structure and stability...

5. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   Published

   Dec. 3, 2020, 2:15 p.m.

   ...tropism. Introducing changes and/or short peptides sequences into these variable loops is one method for changing...

6. Cre-ating New Methods for Site-specific Recombination in Drosophila

   Type

   Blog Post

   Published

   May 12, 2015, 1:32 p.m.

   ...complete or near-complete excision of the stop sequence. In contrast, Dre recombinase, the close relative...

7. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

   Type

   Blog Post

   Published

   Sept. 15, 2014, 6:05 p.m.

   ...have figured out how to download a bit of virus sequence to enable them to recognize and fight it forever...

8. Antibodies 101: Introduction to Antibodies

   Type

   Blog Post

   Published

   Jan. 19, 2021, 2:15 p.m.

   ...searching for. Antibodies may recognize a linearized sequence of amino acids, or only in their 3D conformation...recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity and sensitivity...

9. Viral Vectors 101: AAV Variables That Matter

   Type

   Blog Post

   Published

   June 1, 2023, 1:15 p.m.

   ... (2016). Expression mediated by three partial sequences of the human tyrosine hydroxylase promoter in ... J., & Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven...to read your experimental output; and (4) the consequences to your experiment if there’s off-target expression...

10. Some Like it Hot: Thermostable GeoCas9

    Type

    Blog Post

    Published

    Sept. 14, 2017, 12:40 p.m.

    ...SpCas9 GeoCas9 Size 1368 AA 1087 AA PAM Sequence NGG CRAA (R = A or G) Spacer Length 20 nt...

11. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    Published

    April 25, 2019, 3:01 p.m.

    ...resolution imaging. mNeonGreen also has little sequence identity to other common fluorescent proteins ...

12. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type

    Blog Post

    Published

    March 15, 2018, 12:59 p.m.

    ...the names imply, PAM-out designs have the PAM sequences on the extremes of the targeted region, whereas...

13. Understanding GPCR Signaling Using TRUPATH

    Type

    Blog Post

    Published

    Dec. 14, 2020, 2:15 p.m.

    ...protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016...

14. Hot Plasmids - October 2022

    Type

    Blog Post

    Published

    Oct. 6, 2022, 1:15 p.m.

    ...saturating mutagenesis on 21 positions in the protein sequence of the predecessor GEVIs (called ASAP 1 and 2)...

15. Plasmids 101: Gene, enhancer, and promoter trapping

    Type

    Blog Post

    Published

    May 2, 2023, 1 p.m.

    ...Unifying the known and unknown microbial coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org...

16. Addgene Begins Distribution of Recombinant Antibodies

    Type

    Blog Post

    Published

    March 28, 2022, 4:30 p.m.

    ...community-building. The transparency begins with sharing sequence information for the antibodies in our catalog,...

17. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type

    Blog Post

    Published

    May 21, 2020, 1:15 p.m.

    ..., recombination sites. These sites are target sequences of PhiC31. In the SPARC system, attP sites are...

18. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...the molecule directly into the protein’s peptide sequence. Thus, all synthesized target protein molecules...until they bind a 6-residue tetracysteine tag (sequence: CCPGCC) that can be inserted into the target ...

19. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...complete spectral information, accession numbers, sequences and lineages, FRET efficiencies, additional references...

20. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type

    Blog Post

    Published

    Jan. 25, 2018, 2:09 p.m.

    ...which binds to a small (< 20 amino acid) AviTag sequence in a protein and adds biotin to a lysine residue...