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TypeBlog PostPublishedSept. 25, 2018, 2:39 p.m....accounts for 10,000 or 1%! — BryanRoth (@zenbrainest) August 21, 2018 As Addgene grows, we have been able to...distributing ready-to-use viral preps in 2016 and we just reached our 10,000th viral vector shipped! In the...
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500,000 Plasmids Shipped and Counting!
TypeBlog PostPublishedMarch 26, 2015, 3:24 p.m....plasmids, storing them, providing quality control and customer support, making plasmids easy for you to find ... succeeds because of our amazing community. The trust of our depositors to allow us to help store and ... -
Addgene's Website Update
TypeBlog PostPublishedApril 8, 2024, 2:15 p.m....done straight from the new search bar, but the menu just above it can help you quickly and easily navigate...stands out better on the page, or that the text is just a little bit faster to skim through. Users with ... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog PostPublishedJune 16, 2020, 1:25 p.m....consequence, the preparations for this technique must be larger scale, to allow the extraction of enough...acid and chemical contaminants, these DNA samples must also be entirely endotoxin free. Endotoxin is a ... -
Making Career Development an Integral Part of the Science Training System
TypeBlog PostPublishedFeb. 25, 2021, 2:15 p.m....the project and the findings were captured in this just released report “Enhancing Dissemination of Evidence-Based...continue attracting and launching science talent we must ensure that career skills training is a required... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog PostPublishedNov. 10, 2015, 3:30 p.m....determine its expression level without the need for a custom antibody. There is one major caveat - a tag may...localization and/or function, so each tagged protein must be tested carefully to ensure it retains the attributes... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m....fellow blogger Caroline LaManna put it well: "Wow, custom mutations in monkeys already. I know we've discussed...sequences that Cas9 can target." As further proof of just how big and mainstream the news on CRISPR has gotten... -
Scientist Networking: What is an Informational Interview?
TypeBlog PostPublishedJuly 1, 2014, 3:42 p.m....networking has gotten a bad reputation (as in…”I just hate networking”). Scientists enjoy learning new...Franklin Effect, most people actually enjoy helping (just ignore the people too busy to respond to you). You... -
Top 10 Open Science Developments of 2013
TypeBlog PostPublishedDec. 19, 2013, 3:46 p.m.... data, serve society and patients, and respect industry concerns.” 5) In the aftermath of the Supreme... breast and ovarian cancer risk directly from customers. 6) An analysis of more than 10,500 microarray... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....X-CHIME, a suite of four systems which utilizes custom mouse strains and lentiviral expression vectors....H. (2019). PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m....Vampirovibrio). What’s left of the algae cell is just the cell wall and cytoplasmic membrane. Vampirovibrio...fish (Lutjanus guttatus) by a parasitic isopod (Crustacea: Isopoda)." Copeia 1983.3 (1983): 813-816. Gilbert... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog PostPublishedJuly 3, 2014, 3:48 p.m..... If you were there, you might have even seen me bust a move to “Call Me Maybe.” After cocktail hour, ...humble beginnings as an operator of a printing press. Just as her grandfather used the printing press to make... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog PostPublishedDec. 17, 2013, 2:47 p.m...."The greatest good you can do for another is not just to share your riches but to reveal to him his own...becoming scientists. It takes us a decade or more for just the “training” portion of our careers. In that time... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m....find what works and what doesn’t while engaging in robust discussion with others in the IBEX community. ...resource for anyone performing multiplexed imaging, not just IBEX. The IBEX Imaging Community’s vision is an ... -
Working 9-5: an Addgene Co-op
TypeBlog PostPublishedJune 2, 2022, 2:15 p.m.... work experience I had was in the food service industry, which followed a very different schedule. Fortunately...make sure people feel connected. As I became accustomed to the routine, I realized that the “9-5” was... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog PostPublishedJune 26, 2017, 1:31 p.m....each other, or are friends, are included in this cluster analysis. The closer two dots are to each other...(blue) and social (orange) scientists largely cluster by discipline, suggesting that scientists tend ... -
9 Tips to Achieve Success in Academia
TypeBlog PostPublishedApril 1, 2014, 5:33 p.m....with full force. George Church 1. “Initial and sustained motivation is a huge component of success.” 2...from passion True success evolves from heart and hustle. Each PI emphasizes the importance of passion, ... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog PostPublishedMarch 15, 2018, 12:59 p.m.... oriented in comparison to each other? The gRNAs must target different strands to create a DSB, but this...accomplished with either a PAM-in or PAM-out orientation. Just as the names imply, PAM-out designs have the PAM... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The enzyme-based...at particular genomic sites when in fact they are just non-specific or indirectly interacting. The CUT... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....the gene, we can easily annotate it by making a custom feature in Snapgene. This saves us time as we are...restriction enzymes, but you will also be able to create custom annotations and design additional experiments like...