We narrowed to 397 results for: ARA-2

...the system outlined in Schmid-Burgk, J.L., et al (2). In this post, I’ll cover two great applications ...molecularly evolved from mNeptune2 through 33 mutations and 2 deletions. CyOFP1 has a higher quantum yield, brightness...luciferin 24 hours later demonstrated a more than 2-fold brighter signal from Antares as compared to Fluc...PMID: 22894855. PubMed Central PMCID: PMC3501149. 2. Schmid, J.L., et al. (2016) CRISPaint allows modular...create a calcium sensor with the following characteristics: No photoexcitation High Ion specificity High...

...phages but to also destroy the phage upon re-exposure (2, 3). More specifically, specialized Cas proteins snip..., accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/Cas moieties carry out these functions...length and paste them into the CRISPR sequence. Separate Cas proteins then express and process the CRISPR...of more than 40 cas gene families have been characterized. To date, 83% of archaeal and 45% of bacterial...

...one injection step! The entire protocol takes about 2-3 weeks but requires less than eight hours worth of...for C. elegans genome modification. Figure 2: Schematic from Dickinson et al. (2015) illustrating... is inserted at the 5’ end of a gene, the SEC separates the targeted gene from its promoter, creating ...Dickinson et al. tested this system across 7 genes in parallel; when they injected 60-80 animals per gene, they...

...parts: (1) β-strands 1-9 (β1-9) and the one ɑ-helix, (2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each...activating caspase-3, and detected fluorescence within 2 to 5 hours, a time consistent with previous methods...necessary for fluorescence. When these parts are separated from each other, the protein cannot fluoresce....fluoresce. When β10 and β11 are linked together, but separated from β1-9 they will rapidly recombine with β1-9...on protease activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP...FlipGFP were redesigned so that they are parallel to each other and thus can no longer fit with β1-9. They...dimerize together and force the strands into a parallel conformation. Between β11 and one of the linkers...

...is always best to have options.      Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid...the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids...Susanna Stroik The Doudna lab’s recent work characterized a new tool for RNA targeting, with a leg up ... multiple antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit...observed high fluorescence resilience to these preparations. After crystalizing the hfYFP structure, the...

...(you generally don’t want more tag than protein), 2-3 tags is increasingly common. Tandem affinity purification...TEV cleavage site (more on that in a moment), and 2 ProtA IgG-binding domains. TAP has since come to encompass...found in this paper (available at Addgene).   Table 2: Protease recognition sites commonly used with tags...for your protein. If you’re working with an uncharacterized protein, or a protein for which a good antibody...

...preexisting resistant subpopulation 2. Resistant cells among the separate replicates would have different ...medicine 21.5 (2015): 440-448. Pubmed PMID: 25849130. 2. Hata, Aaron N., et al. "Tumor cells can follow distinct... population using the library, propagated it, separated it into roughly identical replicate groups, and...depiction below): 1. Resistant cells among the separate replicates would contain the same labels - i.e...approach is in contrast to the current treatment paradigm where resistant populations are treated as they...

...vector:insert ratio is used in the DpnI digestion and 2 µL of digest product are used in the transformation...Biology 38 (2016): 145-154. PubMed PMID: 27391134. 2. Li, Chaokun, et al. "FastCloning: a highly simplified...FastCloning, you PCR both the vector and the insert in separate reactions and then combine the products and digest...

...recognize Fab fragments from the same species and 2) these fragments will be more likely to bind (and ...epitopes recognized by the secondary.   Figure 2: There are a few common solutions to avoid background...biorender.com. Proper tissue processing and sample preparation can also help reduce the levels of endogenous...

...Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan...against invading DNA. In 2014, Swarts et al. characterized Argonaute from Thermus thermophilus (TtAgo),...