We narrowed to 904 results for: CAN

...Preprints can be read freely by anyone and are typically free of charge for authors to post. They can be assessed...materials available to the community significantly sooner. Plus, authors can include the Addgene ID for their...them as best you can! At the time of writing, Mike Lacy was also employed by the American Society for Cell...sharing results faster and more broadly, scientists can receive recognition for their efforts, inspire future... preprint as evidence of their productivity that can be shared with hiring and review committees, as opposed...comments, reviewers’ comments, and author responses can be shared directly alongside the preprint while the...awaiting a final publication in a journal.  Preprints can be one tool in a combination of strategies for your...

...going to take a look at a very interesting tool that can be used for creating (excuse the pun) specific, targeted...Cre-lox? The Cre-lox system is a technology that can be used to induce site-specific recombination events...recombination event between two loxP sites, which can be located either on the same or on separate pieces...resulting in the reciprocal crossover event. This event can have three general outcomes based on the location...translocation event is generated at the loxP sites. How can I use Cre-lox? The Cre/lox system is a well-established...selection process. By floxing the selection marker, Cre can be used to easily perform this eviction. Regulated...with tamoxifen or doxycycline), the Cre recombinase can be expressed only in specified cells or at specified...

...factors can also be integrated into the bacterial chromosome, but when encoded on plasmids, they can promote...around us! A plasmid is a small piece of DNA that can replicate independently from its host’s chromosome...we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA passengers. Most natural...keep them around and even help them spread. They can be inherited by daughter cells during cellular reproduction...reproduction, similar to the host’s chromosome, or they can spread between hosts through horizontal (or “lateral...lateral”) gene transfer. Horizontal gene transfer can occur naturally through transformation (cellular uptake...lab specimens — there is evidence that lightning can also promote gene transfer in soil bacteria! (Demanèche...

...rAbs. Production Recombinant antibody production can take place in bacterial, yeast, plant, or mammalian...faster-growing than mammalian cells, but mammalian cells can perform human-like post-translational modifications.... (L'Abbé et al., 2018).  Recombinant antibodies can be produced through a single or dual-plasmid transfection... The antibodies bind the immobilized ligands and can be eluted once the other materials have been washed...preferred buffer. If that’s the case, a buffer exchange can be performed using a desalting column or an ultrafiltration...extinction coefficient of IgG is around 1.35, though this can vary depending on the specific amino acid sequence...protein standard. The density of the antibody bands can be measured against the protein standard and the ...

...relocation plans. They can be skills-based: if you're switching fields, you can use your cover letter ...cover letters can be a barrier for qualified applicants. In short, while a cover letter can’t replace skills...reproducible assay. That information can make you a much stronger candidate!  Additional uses The cover letter...transferable skills make you a good candidate for the position. You can also address any missing experience...cover letter won’t hurt your application, but it can certainly help you stand out.  In some fields, a ... application. Even within an organization, there can be variance. At Addgene, our content team, where ...have ddPCR experience. In your cover letter, you can expand on that by describing how you developed and...

...out of a complex mix. Using this technique, users can look for the presence or absence of a protein, determine...sites free to capture the target protein. The beads can be collected by centrifugation (agarose beads) or...recombinant protein, Protein A/G, was developed, which can be used for most antibodies compatible with either...for binding sites. In cases like these, antibodies can be directly conjugated to agarose or magnetic beads... not require centrifugation between washes which can cause loss of beads, break weak protein interactions... to use will vary between capture antibodies and can be determined through titration. Unbound or weakly... are removed through washing, the target protein can be eluted. The elution method will vary depending...

...traditional restriction enzyme cloning. Instead, you can choose a molecular cloning technique that will work...the appropriate entry and destination vectors, one can use Gateway to clone a gene of interest into a variety...Under certain conditions, the attL and attR sites can recombine, leading to the excision of the phage from...modified versions of the att sites so that scientists can easily clone in their desired DNA sequences. Gateway...is toxic to the cell, Gateway cloning efficiency can reach >99%.   STEP 3: Express your Gene of Interest... via sequencing or restriction digest! Then, you can transform or transfect the cells that you want to...entry clone with your DNA sequence of interest, you can move this DNA fragment across any expression system...

... that dimerize can form dynamic contacts that bring apposing membranes together and can wrap the membranes...noncovalent dimers. This property of EGFP can lead to significant artifacts. If you're using green fluorescent...families, which cannot form heterodimers (i.e. EGFP and mCherry). These simple strategies can help you make...homogenously distributed throughout the volume and can freely tumble in 720°. However, if the molecules ... FRET biosensors that contain two dimerizing FPs can exist in an open inactive state, a closed active ...FPs will collide with each other. The consequences can be dramatic. For a membrane POI localized to the ..., the normally spiderweb-like pattern of tubules can be grossly distorted into dense stacked membranes...

... variations in how one can gate, select, and analyze data. But now that you can read a FACS plot and follow...literature. Data reported from flow cytometry experiments can be a little challenging to understand if you’ve never...them it's helpful to have done flow first… which can send you in an endless loop. Let’s break out of the...users to analyze single cells in a population. It can be used to measure protein expression, identify rare... by the researcher, based on their controls. You can usually tell where this point is by looking at the...the cell populations as a guide  (Figure 3.) You can see that in our dot plot, we have A-B- cells, A-B...markers. There are various types of SSC and FSC that can be used for this purpose.  Pro tip! The forward scatter...

...nests so can be studied over time. They recognize individual faces (I guess of other wasps) so can be used...This black footed slipper snail can be manipulated by injected RNA and can express fluorescent proteins....organism, but researchers are optimistic that this can be done for most, if not all, of these new experimental...is designed to expand the number of systems that can be studied by providing support "for development ... and educational programs for K-12 students. You can see why I was excited to be invited to attend. My...reagents from these emerging organisms so scientists can easily find the useful new reagents that come out... nest on your house. Their nests are open so you can see them and even feed them RNA to do silencing in...