We narrowed to 614 results for: CaS;

...catalytically inactive form of Cas9 known as dCas9 (Jinek et al., 2012). Like Cas9, dCas9 can bind to a specific...targeting gRNA. But dCas9 does not cleave the DNA. Much of the research using dCas9 has focused on transcriptional...mammalian cells by expressing dCas9 with an N-terminal 3xFLAG tag (3xFLAG-dCas9) and a gRNA targeting their...editing, numerous discoveries have been made using the Cas9 nuclease in the absence of genomic alterations. ...activators (Qi et al., 2013). However, the fusion of dCas9 with a protein tag allows for the isolation of a...genomic region using the CRISPR system, consisting of dCas9 and targeting gRNA, to identify molecular interactions...3xFLAG tag, allowed these researchers to isolate dCas9 with the bound genomic locus, which they then confirmed...

... un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks ... express both SpCas9 and your gRNA in a variety of mammalian cell types. Purpose  SpCas9 This plasmid ...tools came out including the single base editors, Casilio, CombiGEM, and a variety of pooled libraries. Not... as is obvious from our most requested plasmid, SpCas9 is still going strong as the basis for many genome...further ado, the most request plasmid in 2016 was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last...2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though a little ...plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified version of CAG promoter...

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...great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires... CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment...potent Acr-LOV fusion protein inhibitor CASANOVA for “CRISPR-Cas9 activity switching via a novel optogenetic...AcrIIA4.” CASANOVA responds to blue light quickly. Within minutes of inactivating the CASANOVA complex,...CRISPR systems including the popular S. pyogenes Cas9 (Rauch et al., 2017, Hynes et al., 2017). However..., they have developed a system to control CRISPR-Cas9 activity using blue light (Bubeck et al., 2018)...., inhibiting function of Acr. This allows CRISPR-Cas9 to function on its target.  They name their most...

... AAV delivery of Nme2Cas9 with gRNA cassette Erik Sontheimer’s lab identified a Cas9 protein from Neisseria...Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans...paper in Communications Biology Find the Cas9-PCV and PCV-Cas9 plasmids Selective curbing of unwanted ...After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to an Mxi1 repressor...gene. Read the paper in mSphere Find the dCas9 and dCas9-Mxi1 plasmids If you have a new CRISPR tool...library Covalent tethering of DNA repair template to Cas9 Homology-directed repair (HDR) can be used to make...oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex via the fused HUH endonuclease ...

...range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely...sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to...recognize the target. Osamu Nureki’s lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for the third ...NGG. In addition to the engineered SpCas9, they also fused the SpCas9-NG with the activation-induced cytidine...  The Kanemaki lab previously developed a CRISPR/Cas9 based method to tag endogenous proteins in HCT116...widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific...cells. The increased versatility of the engineered SpCas9-NG and its variants can be used to engineer specific...

...different Cas12a inhibitors. Some of these anti-Cas12a proteins have broad inhibitory effects on Cas12a orthologs... research.     CRISPR-Cas12b for human genome editing The best studied Cas12b nuclease from Alicyclobacillus...The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C but...Communications Find the Cas12b plasmids  Plant base editing with a Cas9-adenosine deaminase fusion... - this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome editing system is regulated...Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature incompatible...tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor can be used for A•T to G•C edits...

...combining specific sgRNAs with Cas9 or Cas9 derivatives. Unmodified Cas9 causes double stranded DNA breaks... loss of function while nuclease-deactivated Cas9 (dCas9) can be tethered to a repressor (CRISPRi) or ...an inactive RNA-guided DNA binding protein (dCas9). dCas9 is then tethered to a repressive domain, such... issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point mutations in the ...library In addition to inhibiting gene expression, dCas9 can also be used for transcriptional activation....developed to recruit transcriptional machinery to dCas9 and its RNA directed binding site. These methods... fusion of an activation domain such as VP16 to dCas9 or the recruitment of multiple copies of VP16 termed...

...nicking Cas9. A guide directs Cas9 to a site of interest, where it nicks its target DNA (left). Cas9 dissociates...enhance nCas9 (enCas9) EvolvR with a targeted mutagenesis rate ~9-fold higher than the original nCas9, while...error prone PolI3M that’s fused to a nicking Cas9 (nCas9). Similar to mutagenesis with error prone PCR...used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9 nicks and then dissociates...amino acid changes were introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking... a user-defined genomic region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the...diversification by targeting a site of interest with dCas9 fused to the Activation Induced Cytidine Deaminase...

...releasing Cas9. Nanoblades can also deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, ...centrifugation. Gag-Cas9 fusion protein The MLV Gag protein fused to a Flag-tagged SpCas9 is incorporated ...Nanoblades by association with the the Cas9 portion of the Gag-Cas9 fusion protein. A single Nanoblade particle...along with the gRNA and Cas9. An “all-in-one” Nanoblades which carries a gRNA, Cas9, and a HDR donor template... leukemia virus (MLV)-like particles loaded with Cas9-gRNA ribonucleoproteins (RNPs). According to the...generation. Key components of Nanoblades Nanoblades are a Cas9 RNP delivery vehicle that’s based on mouse leukemia...just like the unmodified MLV Gag protein. After Gag-Cas9 is incorporated, the MLV protease, supplied as part...