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...something difficult, be excited about the prospect of working toward a solution. At the end of the meeting, make...person to add a bullet or two about what they are working on. Building this into the agenda ensures that ...items, and Accountability Agendas: Send around talking points for the meeting a day in advance. This gives...Accountability: At your next meeting, start by checking in on the action items from last meeting. Use ...project moving. Use time wisely Meetings are for talking, not reading: Send around relevant documents, like... Executive Director Joanne Kamens FInd Tips on Picking a Research Project Read Other Inside Addgene Posts...

...the day you defend to start thinking about your next step. Start thinking now. Start browsing job postings...in your resume. If the job posting says “French-speaking is an asset,” mention “speaks French.” Employers...groups? Perhaps you had some supervisory experience working with undergraduate students? On top of that, perhaps...universal. Some universities even have officers working specifically to assist graduate students in job...postings and salary expectations public, so start by looking at these university postings to give yourself a...

...you build your lab team and foster an effective working environment? Tom Ellis was offered a start-up package...ideas, and ways to improve the lab. “An effective working environment for research is totally dependent on...communication amongst the team so getting everyone talking to each other cannot be undervalued.” Connie Cepko...of slowly building up your lab and selectively picking the first couple of hires to build a strong, collaborative...resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc... Dr. George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists...

... these files to your working directory (the folder that you are currently working in) every time you use...notebooks just won't cut it for bioinformatics. All kinds of biologists are finding themselves using computational... task will keep you from repeating your work or making similar mistakes in the future. Stay organized...comment in C++ and Java Command line history: Tracking command line history by recording all commands...system that does is through trial and error, and by asking for tips from others. Comment below to add your...

...Blog Mentoring for Scientists Series. I have been thinking a lot about Mentoring for over 10 years. Many ...Cepko to learn about her mentoring style. While working on this project, I read the book “Every Other Thursday...Form your own peer mentoring group In my travels speaking to grad students and postdocs I have started to...example, a group of people who are all interested in making a transition. Schedule your first meeting and get...breakfast once a month. All participants must commit to making the meetings a scheduling priority (and last minute...career paths and get their advice. Attend a local networking event together and help each other practice meeting...

...like multi-color imaging (Figure 2). If you’re working with purified proteins in vitro, you can even use...tags makes updating your labels easy! Instead of taking the time to clone a new construct for every new-and-improved...time points.   Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins...developed over the years; improving the labeling kinetics or the fluorophore’s microenvironment to enhance...A., Juillerat, A., Heinis, C., Corrêa, I. R., Kindermann, M., Beaufils, F., & Johnsson, K. (2008). An ...Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling...Selecting Fluorescent Dyes and Ligands GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic...

... be useful. Mainly because, well, antibodies are kind of hard to see. To solve this issue, researchers...post, where you can find some additional tips for working with these tools.          Enzymes An equally, ...properties allow for efficient signal amplification, making biotin conjugated antibodies good options for detecting...lead perfectly blissful, productive lives without thinking about these chemical reactions. After all, there... are ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...antibodies’ performance after the reaction.  If you are looking to conjugate antibodies yourself, there are kits...

..., consider making you own DNA ladders with these plasmids from Penn State Need help picking the right ...cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell culture media. Their...HA staining is weaker than reporter staining. Picking the right reporter for your RANBody While all three...staining GFP, RFP, H2A2B   Are you ready to start making your own RANbodies? Great! All of the RANbody plasmids...learn more about nanobodies Additional Resources Looking for primary monoclonal antibodies to use with the...

...heavily with other skills such as critical thinking, decision making, creativity, or data analysis. Conveniently...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...you spend time in a lab learning techniques and taking on independent projects, you will inevitably develop... skills passively. But I have always found that taking a conscious or active approach can be hugely helpful...are, while also practicing systematic, logical thinking.  If you are already a lab veteran at this point...

... Research. On December 1st 2016, many postdocs working more than 40 hours per week could expect to see... salaried workers receive overtime payment for working more than 40 hours per week increasing from $23,660... postdocs were at institutions focused only on tracking hours, and 22% of postdocs were at institutions...going ahead with their planned raises, some are taking the opportunity of the lapsed mandate (or are being...University of Massachusetts Medical School. We are working with postdocs at other institutions to see if we...that many postdocs already made plans and budgets working around issues such as childcare using money from...