We narrowed to 605 results for: RAN-1

...how scientists connect on Twitter that I realized 1) a lot of scientists are using social media professionally... but my consistent writing for the Addgene blog (~1-2 blog posts per month) and use of Twitter and LinkedIn... have tips for using social media as a scientist. 1. "5 Ways Scientists SHOULD Be Using Twitter" podcast...of interest. Hopefully through these links, I’ve: 1) convinced you social media can positively impact ... to research potential future employers and to arrange informational interviews. 4. “Tips for Scientists...

...intended.)  Steps to clear writing Let’s use Figure 1 as our example. Remember, our goal is to use words...information as sighted readers.    Figure 1: An image from AAV Purification by Iodixanol Gradient...are they hoping to get from it? The image in Figure 1 appears on our protocol page for AAV Purification ... share with the rest of the workshop.  For Figure 1, Addgene chose this alt text: "The left panel is a...resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad Students Writing Scientific Manuscripts...

... vectors are: 1) the potential generation of replication competent virus [usually HIV-1]; and 2) the potential...systems have been developed over the years based on HIV-1; however, the 1st-generation vectors are never used...which has been shown to repress expression of HIV-1 LTRs (more information is available here). 2nd vs...considered. Q2: Can I use lentiviral transfer vectors for transient transfections? A2: Technically, yes. Will ...produced by co-transfecting three essential ingredients: the lentiviral packaging vector, a transfer vector including...specifically for transient expression (find some useful backbones here) or from viral transduction. While not...3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors based...

...perform, at minimum, two transformations after a ligation: 1. Control Transformation containing the ligation...competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction into competent ...well, so let’s go over the details step-by-step. 1. Digestion Set up restriction digests for your insert...recipient plasmid : insert ratio” of approximately 1:3. Since the number of base pairs for each varies,...result in few colonies on both plates (unsuccessful 1), in a vector alone plate with many more colonies ...contamination from uncut plasmid. 4. Transformation Transform your ligation reaction into your bacterial... how many colonies you should expect in the transformation due to background re-circularization and contamination...

.... Table 1 provides an overview of pooled library protocols you can use as a reference. Table 1: Examples...backbone might be more difficult to transform.    Figure 1: Factors to consider when creating a...etc. The DNA amount can be anywhere from 10 ng to 1 μg of DNA. It often correlates with the size of the...libraries, chemical transformations can also be used. Efficiency for chemical transformations is less than that...for your transformation(s). The amount of competent cells you need total and per transformation may vary...the manufacturer’s protocol for transformation and expected transformation efficiency, or more specifically... coverage, since this will help decide what transformation efficiency you need to achieve. Most pooled...

...to accommodate specific volume ranges of culture as their input (Table 1). When you feel like you need ...require large amounts of high quality DNA. Figure 1 illustrates the three grades of DNA you may need based...their relative quality and quantity.   Figure 1: Plasmid preps fall into different categories based...kit for example. These kits are designed to handle ~1–5 mL of liquid culture. Using a higher volume can ...beads and a suitable magnet as you scale up. Table 1: Standard Prep Kit Capacities* Prep size Amount...Amount of culture Approximate DNA yield** Miniprep 1–5 mL 5–50 µg Midiprep 25–100 mL 20–200 µg Maxiprep...BioRender.com. Transformation Grade DNA (low quality/low quantity) – Bacterial transformation protocols require...

...2 (CRY2) and calcium and integrin-binding protein 1 (CIB1) proteins. The goal of both groups was to create... the Sata lab is composed of two fusion proteins: 1) the genomic anchor - an inactive, dead Cas9 protein...combination. The optimized LACE system consisted of: 1) CIBN-dCas9-CIBN, where CIBN is the N-terminal fragment.... crassa) (Kawano et al., 2015). Nicknamed paCas9-1 and consisting of the fusion proteins N713-pMag and...fold-induction of Cas9 activity (16.4-fold). This paCas9-1 light-inducible system was able to recognize the same...flCas9). When triggered by blue light (470nm), paCas9-1 induced indel mutations via NHEJ (frequency of 20.5%...background activity of the system by modifying paCas9-1 using nMagC714 instead of nMagHigh1-C714, generating...

...total dose between 1 x 1011 and 5 x 1011 vg per animal (i.e., 10 and 50 µL of vector at 1 x 1013 vg/mL titer... and 1 x 1012 vg*. Most systemic capsids are easy to produce in high titer, but higher doses (>1 x 1012...titers than AAV-PHP.B (e.g., 1 x 1011 vg/mouse), AAV-PHP.eB can transduce a greater number of neurons ...intravenous gene transfer throughout the non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org...endothelial cell transduction throughout the central nervous system. Nature Cardiovascular Research, 1(4), Article...capsids facilitates enhanced transgene expression in the macaque CNS. Med, 4(1), 31-50.e8. https://doi.org...before using it in an application.     Figure 1: AAV toolkit for targeted gene delivery to the brain...

...2021).   Table 1: Properties of modern NIR FPs     NIR FP Ex, nm Em, nm ECa, M-1cm-1 QYb, %   Molecular...expression level. State-of-the-art FPs (listed in Table 1) were optimized for efficient binding of endogenous...previously reported widely used dimeric iRFPs (Table 1). In general, the monomeric state of a probe leads...iRFPs in cellular and molecular brightness (Table 1).  NIR reporters and biosensors NIR reporters can ...dDetermined as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous...lamps are the best for working in this spectral range. You can also check that no filters and/or cold ...and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...

...attract fish for them to prey upon (Figure 1). Figure 1: The sea anemone's red color can be used ...synthetic biology. Journal of Biological Engineering, 12(1). https://doi.org/10.1186/s13036-018-0100-0 Murakoshi...expression in living cells. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-48604-4 Salih...chromoproteins are just now being developed for a broad range of biological inquiries.  What is a chromoprotein... a gene encoding a blue chromoprotein, after transforming them into your cells, you can simply look for... plasmids (meffRed, eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue...Image: Murakoshi et al., 2019. ShadowR is an orange light-absorbing chromoprotein that Hideji Murakoshi...