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  1. CRISPR Guide

    Type
    Guide
    ...changing the target sequence present in the gRNA. Figure 1: Overview of the basic CRISPR mechanism. CRISPR was...from Staphylococcus aureus ) has a coding sequence ~1 kb shorter than SpCas9 while retaining the same basic...lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included in the gRNA-containing...343–347. https://doi.org/10.1038/s41586-022-04470-1 PMID: 35236982 Bubeck, F., Hoffmann, M. D., Harteveld...doi.org/10.1038/cr.2013.122 PMID: 23979020 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N....efficiency and fidelity. Nature Communications , 13 (1), 1425. https://doi.org/10.1038/s41467-022-29089-8...interrogation by SpRY-Cas9. Nature Communications , 15 (1), 3663. https://doi.org/10.1038/s41467-024-47830-3...
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