We narrowed to 897 results for: ANT

...NgAgo In eukaryotes, Argonaute proteins play an important role in RNA interference, binding RNA guides to...replicated in larger studies, it will represent a key advantage over Cas9, for which gRNA sequence can greatly...aa depending on the species). This feature is advantageous if you’re working with small capacity viral ...via indel formation, this property could be an advantage. However, repair after base excision would alter... Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional...

... laptops and some server space. But what if you want to be an entrepreneur in the health sciences?  Is...The composition of the management team is more important than the product itself -- investors would rather...majority of these meetings acted as a platform for significant company advancement. Be relentless The world ...against you.  As I mentioned, you start at a disadvantage in the health science space if you don’t have... to it. First impressions are everything and you want to leave people remembering your potential as an...

... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...hurdle in front of publication when all they really want to do is get the paper off their desk and move on...only a partial solution.  How do we get where we want to be? The part that is still missing is a clear...management plans and for evidence of sharing for grant renewal—but there is clearly room to do more. If.... Sounds like a win for everyone. If we agree we want to get there, that’s half the battle. Many thanks...

...biosensor tools, we now offer the jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting...basal fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large ...-WPRE 105322  AAV1 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 111262  AAV1 pAAV-hSynapsin1-axon-GCaMP6s...Name 105322  AAV1  pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 105714  AAV8  pAAV-Ef1a-fDIO-GCaMP6s...

...connections Here are a few things you probably don’t want to do: Don’t connect via LinkedIn with strangers... for favors.  Don’t focus on networking with “important” or senior people. Focus on building relationships...times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There is a...4,700 LinkedIn connections. I mention this because I want it to be clear that it is completely impossible ...interest in you. Network with the people that you want to be with. Then, the following up will be easy....

...growing the cells on solid plates, where each transformant forms its own colony, uninfluenced by the growth... from both. These products are then purified, quantified, and sent for sequencing. Analyzing your sequencing...corresponding sequencing quality. Generally, you want to have 100x-1000x coverage of your library: in ...very similar to each other - that’s exactly what we want! At the end of the day, it’s up to you to decide...hope this post has helped clarify some of the important considerations when using, amplifying, and verifying...

...avenues previously unavailable to them. With these fantastic tools it’s now easy to monitor protein expression...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...precisely spatio-temporally controlled as a key advantage over other inducible enzyme technologies. She ...Oligosaccharides (HMOs). HMOs are thought to play an important role in nuturing the development of beneficial...communication skills throughout her training and wants to make her work understandable to people both inside...

...used to negotiate individual MTAs every time they wanted to share materials, and the process was time-consuming...facilitate a fast and agile MTA process. This is important to keep up with the number of requests and deposits...sharing process smoother, more consistent, and more pleasant for everyone, which in turn encourages people ...process.  And you do have options for the terms you want to share your biological materials under! While ...for both research and commercial purposes. It’s important to note that it’s the depositing institute that...

...community, so it is extremely important for us as a company to constantly update our processes and get ...for Fragmentation System and this is the most important part of this newly released prep kit. In their...itself. This was great because it forced workshop participants from different biotech companies to interact...Therapeutics, a biotechnology company that uses neoantigen biology to treat cancer. My partner for the day...

... (Fig. 1). However, once thawed, there was a substantial reduction (from 7.4 to 5.0) in the pH of samples...samples stored in our AAV tubes (Fig. 1). It’s important to note that the change in pH only occurred when...that dry ice storage affected the performance of antibodies, because of effects of pH on protein stability...infectivity of the virus. However, there was a substantial reduction in infectivity after 3 weeks of exposure...Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors. Mol Ther Methods...

...plexWell™’s sequential tagging procedure, the quantities of library molecules for each sample remain highly...plate) will typically have a wide range of input quantities. This variation is problematic for conventional... individual libraries can be influenced by the quantity or quality of input samples. To minimize these...from multiple samples will then also need to be quantified and re-normalized in order to be combined with...proprietary chemistry). This turns out to be critically important for getting adequate sequencing depth and coverage...

...great for lab life. These apps prevent you from constantly searching the interwebs for lab resources, and... Lab Archives by LabArchives The single, most important part of lab life is the lab notebook. Lab Archives...always comes first with research! It is always important to know what kind of reagents you work with in...MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet is in this...iGreenEarth AMINO is a very simple app that packs important biological information, amino acid abbreviations...

... active variant, the phage is not infectious. The editing speed dictates which phage variants can propagate...complete ABE protein. If the phage delivers an active variant of the base editor, a functional gene III product...stringency PACE, resulted in the evolution of TadA variants with much higher base editing activity compared... TadA-7.10 enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold...

...however, did not improve at the same pace as the quantity and quality of the information. As a result, resources...different strategies for finding what they need, and we wanted to support all of them — searching, browsing, drilling... supported and combined?  Luckily, we had the advantage of a very specialized domain of information so...search engine to return only the filters that are relevant to a specific query and category. For example,...that make our new search engine unique. Most importantly, with this new implementation, we laid the foundations...

...choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have ...light-oxygen-voltage sensing domain from the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds...15 kDa), single monomeric variable heavy chain antibody domain.  How do these components interact? When...binding ability of its nanobody partner, so it’s important to insert AsLOV2 at a spot in the nanobody that...tagged, which limits these approaches to use with recombinant proteins. Purifying an untagged protein with ...

...Harvard. Roo wants to simplify access to academic resources among economically disadvantaged populations...graduate students. In order to gain a competitive advantage this cycle, consider the advice below:  1. Eligibility... and lighting for a smooth performance. Most importantly, make certain that everyone you live with is ...increase your competitive edge, they alone, are no guarantee of success. Don’t luck into an internship, prepare...

...most important resource that Addgene provides the scientific community? Katz: The most important resource...beyond. In your perspective, what do you think are important considerations for the future of open science?... on average 20 PhD’s a year and we have a very dominant presence in the biotech sector in both patent ...from Babson College. He is a certified public accountant (CPA), certified information systems auditor ...

...in low penetrant phenotypes that are hard to study. To overcome this, most scientists use mutant animals...leads to significantly stronger phenotypes than standard RNAi protocols without the need for mutant genetic...norovirus is enriched with synbodies--or synthetic antibodies--attached to magnetic beads, allowing the virus...

... autophagy and, in a mouse model led to improved anti-cancer immunosurveillance and reduced tumor mass...on novel cancer therapeutics such as  (Chimeric Antigen Receptors) CAR T cells and PD-1 modulators. An ... et al. "Caloric restriction mimetics enhance anticancer immunosurveillance." Cancer cell 30.1 (2016):...PubMed PMID: 27411589. 2. Todoric, Jelena, Laura Antonucci, and Michael Karin. "Targeting inflammation in...: eaal3118. PubMed PMID: 28495700. 4. Zelenay, Santiago, et al. "Cyclooxygenase-dependent tumor growth...

...several advantages over traditional vaccines, including 1) cellular responses in addition to antibody response...but this step can be engineered to be anything you want delivered to your target cells. This delivered DNA...the target cell or transiently expressed. It’s important to note that for all the applications we discuss...Gene expression control Researchers are always wanting to manipulate gene expression – turn it up, turn...

...base editors in vivo and achieved therapeutically relevant editing efficiencies in the mouse brain, liver...voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage indicators (GEVIs)...McCormick lab generated knock-out and knock-in mutants, determined protein expression levels from different...the CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID: 30819783. Light up CreLite...PIF6, phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In the presence of red light...

...not be important for a good friend, but if you are making a professional inquiry, you always want to read...many academic scientists are starting to see the advantages of using social networking to start, build, and...the easiest ways to help is to connect people that want to meet or that you think might be helpful to each...Do not post or comment on anything that is not relevant to business or volunteer organizational work. ...

...can be both sorted using FACS and isolated with antibiotic selection. The team tested this system in vitro...the addition of standards, the assay is semi-quantitative and can approximate mRNA concentration within...bacteria of clinical significance and four clinically relevant host biomarkers. The lab validated their platform...host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible ...microbiome responds to infection, dysbiosis, and antibiotic treatment. To facilitate these studies, Pamela...

...slides, handouts, and resources were hosted on the Plantae website blog post written by Ben Schwessinger, ...I realized that not all the materials would be relevant to everyone at this specific conference, and I...’s How-to-guide covers everything that you would want to know before, during, and after organizing such...was also recently awarded a Mozilla Science mini-grant, which will fund a project to support a dedicated... and impactful opportunities - There are many brilliant minds out there, but how do you get them to connect...

...added using PCR-based amplification (Option 2). Advantages of Golden Gate cloning Golden Gate cloning is...potential clones. Golden Gate assembly has a few advantages over other cloning methods. Exonuclease-based...destination vector, making multiplexing easy.  Disadvantages of Golden Gate cloning Golden Gate cloning is...may not be amenable to "domestication", you might want to consider using an alternative method like Gateway...Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

...other references to scientific literature to substantiate the facts upon which it builds. This means you...your area. When you are in ‘writing-mode’ for a grant or a scientific manuscript, the reading will likely... you an overview of the topic and point you to relevant primary research articles. Then just start reading...key words and download what seems interesting or relevant. Harness the power of the Google search engine...Google Scholar will send you daily updates with relevant literature. Organizing the literature As a scientist...

...‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the more loosely bound...acetyltransferases or CARM1 methyltransferase. This attendant signaling feature afforded by coactivator complexes...comprehensive datasets that collectively address many important and fundamental questions in nuclear receptor ...states, their circadian regulation, as well as in important physiological states such as adipogenesis and ...coregulators. We look forward to growing this important relationship with Addgene for the benefit of the...

...altogether different. They are filled with blind anticipation. Their trust in the quality of the content is... manuscript could only have been viewed as a significant improvement. As a result, the software piñata...those engaging in CRISPR design, but it is a significant hurdle made worse by an ever increasing marketplace...for this purpose. This second area is extremely important and given the lack of attention to it, it is an... interact with the software. This category is important for users wishing to select tools based on their...Matchmaker, it provides a list of terms I think are important to define for the CRISPR software space. It is...

...Outbred strains of mice are useful for comparing dominant and recessive traits and their impact on diseases... case, a positive selection marker such as an antibiotic resistance gene is also included to select for... are injected into a blastocyst which is then implanted into a female animal for gestation. Nucleases ...are microinjected into a zygote, which is then implanted into a female mouse for gestation. All three can...nucleases to generate an engineered mouse model has advantages over the injection of a targeting vector alone...

...these communities, which is in turn crucial if we want to make sure that everyone can be informed and empowered...individual consumers of technologies and as societal participants in the political process. My role at pgEd I ...who are interested in such careers should take advantage of their academic environment and attend seminars...either giving or running these events will be important both in preparing you for future work in these...communicate your work to a non-technical audience is an important skill for every scientist. After all, scientific...