... neurological defects. Nature 550:398–401 . https://doi.org/10.1038/nature24058 Tillotson R, Bird A (2020...can find the reagents and resources they need to cure Rett Syndrome.  If you’re new to Rett Syndrome, ...walking. They may exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand...gene therapies to clinical trials in 2021. Figure 1: Schematic of the human MECP2 locus and MECP2...

...formation requires a fully folded beta-barrel structure, followed by an intramolecular reaction that generates...shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is maintained...specific phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production can be detected by using a MS2...specifically detects a specific RNA stem loop-structure (Querido and Chartrand, 2008). Finally, a nuclease-deficient...Quantitative assessment of fluorescent proteins." Nature methods 13.7 (2016): 557. PubMed PMID: 27240257...

...such as the brain, transparent so that the 3D architecture of a fluorescent neuron is visible. Chemical... al. "Improved tools for the Brainbow toolbox." Nature methods 10.6 (2013): 540. PubMed PMID: 23817127...neural circuit analysis in Drosophila melanogaster." Nature methods 8.3 (2011): 260. PubMed PMID: 21297619.... arises from expansion and dedifferentiation of mature pigmented melanocytes." Cell stem cell 21.5 (2017...of fluorescent proteins in the nervous system." Nature 450.7166 (2007): 56. PubMed PMID: 17972876. Pan...

...interactions found in nature. Once the bond is formed it is unaffected by changes in pH, temperature or organic ...Discovery of the biotin-avidin interaction   Figure 1: Biotin is enriched in egg yolk while its binding...affecting the avidin binding site. With all of these features it isn’t surprising that scientists began to exploit...with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534–545. PubMed PMID:18323822...

...both targeted alleles when gene editing.   Figure 1: Techniques to improve editing efficiency. ... RNAs wisely (and let them cut close).   Figure 2: Relationship between cut site to mutation distance... one or the other on each target site.   Figure 3: Optimal cut site to mutation distances for heterozygous... and heterozygous mutations using CRISPR/Cas9." Nature 533.7601 (2016): 125-125. PubMed PMID: 27120160...scarless genome editing in stem cells using CORRECT." Nature protocols 12.2 (2017): 329-354. PubMed PMID: 28102837...

...standardized leader peptide prevents secondary structures in the mRNA encoding your gene of interest from...in-vitro assays: Koch, Daniel, et al. "A pull-down procedure for the identification of unknown GEFs for small...monitoring sequence dynamics under various selective pressures: Miller, Corwin, et al. "Adaptation of Enterococcus...applications of PUF protein fusions reported in the literature include RNA detection, RNA cleavage, and translational...endonucleases with customized sequence specificities." Nature communications 3 (2012): 1147. Cooke, Amy, et...

...few hours at low cost with restriction mapping (Figure 1). Though some labs may prefer to send DNA directly...computer program) can then reproduce the DNA sequence (Figure 2). Accuracy and accessibility led to the widespread...reactions and may use Sanger sequencing. Looking to the future Southern blotting, restriction mapping and sequencing...sequence an entire genome for under $1,000 (10). The future of sequencing should bring efficient, low cost ...large amounts of DNA quickly and accurately. We're sure to see many improvements in a variety of biological...

...accumulated over the years using them. Indeed, the future of mouse work is bright as it is now easier than... 2013 (Cong et al., 2013)! Haoyi got his first exposure to CRISPR when he used this new technology to ...pyogenes. An alternative Cas9, from Streptococcus aureus, is as effective at editing mouse embryos as Cas9...appreciate the fact that it was first discovered in an obscure bacterium and that we still have much to learn ...Production of Gene-Modified Mice using Staphylococcus aureus Cas9. Sci Rep 6: . https://doi.org/10.1038/srep32565...

...to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, as it relies on...promoter or exonic regions. If the cell’s chromatin structure undergoes significant rearrangement (such as when...specific genetic loci of integration [8].   Figure 1: Mechanism of the piggyBac™  transposase/transposon...cells with a Footprint-Free™ genome edit.   Figure 2: Editing DNA using the piggyBac™ transposon selection...26299960. PubMed Central PMCID: PMC4559351. 8. A. G. Uren, H. Mikkers, J. Kool, L. van der Weyden, A. H. Lund...

...mutagenesis. Chapter 6 - The future of FRET and fluorescent protein tools. Future developments in microscopy...the Addgene Blog Visit our Fluorescent Proteins featured topic page Read Joachim's Series on Choosing the...Protein Plasmids 101: Visualizing Subcellular Structures and Organelles Resources at Addgene.org Find...

...started with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly...antibodies or viruses. If you’re new to tissue culture, check out this video for some tips and best practices...practices for getting started with tissue cultures. To keep up on all of the latest protocol videos...

...recommend you read the article to ensure you understand the features of the plasmids, how they were created... to the PubMed Preferences and Filters pages, be sure to check out the other “LinkOut” badges you can ...Addgene Blog Get Tips on Keeping Up with the Literature How to Write a Scientific Review Article Tips...

...Massachusetts that means it’s time for near-freezing temperatures, our first of the season snow flurries, dusting...holiday season! It is that time of the year again: miniature #Christmas tree goes up at home!This year with...December 4, 2020 Here’s how to enter: Post your picture or video for either contest on Twitter or Instagram...follow us on Twitter and Instagram as we share pictures of our decked out spaces.       ...

... plasmids shipped daily may be easy to picture (see the picture of our shipping room above), but as Joanne...amongst the lab team, shipping team, and others to ensure that everything will ship out in time - from assembling...said during our celebratory lunch, it’s hard to picture one million plasmids. How much space would one ...

... of a scientist was like. The word “scientist” conjured up images of men like Albert Einstein and Bill... make decisions about what we want to do in the future. How do I get started with research? I had no idea...personal interests While it’s perfectly fine to be unsure, it helps to have a vague sense of what kind of..., even if a question is a very basic one, making sure that you have a good fundamental understanding of...goals helped me reflect on what I wanted for my own future. Informational interviews with people who have ...

...lucky enough to have an imaging core or tissue culture core, you are looking at a process-oriented team...come across one. Also, remember, one important feature that all projects have in common - and by association...is a blog post, so here are just a few critical features based on my own experience: Leadership Few things...certainly necessary, but equally important is making sure all team members understand what roles they’re meant...well, you can learn from the experience and make sure your next team works better. In fact, don’t wait...

...breakthroughs and undermine critical research infrastructure that has taken decades to establish.These funding...materials that might otherwise be lost amid lab closures or project terminations, ensuring that scientific...grant applications, addressing the new funding structures and indirect cost caps more efficiently. Offering...can navigate this difficult period together. The future of scientific progress depends on our collective...

...Exciting news! Addgene recently rolled out a new feature on our plasmid pages - links to articles citing...screenshot below to see what I mean. Additional features Once you've clicked on the "# References" link...article abstract and more. We've also included a feature to help save you from performing any unnecessary...domains" publication.  If you want to see this new feature in action, click here to explore the TetO-FUW-OKSM...

...Doudna and Eva Nogales presented the first detailed picture of Cas9's three-dimensional shape via x-ray crystallography...Cas9's structural details. "Understanding this structure may help us engineer around the current limitations...in Feng Zhang's lab, in a press release. "In the future, it could allow us to design versions of these ...University summed it up well in that New York Times feature when he said, “All of this has basically happened...

...Live-cell measurements of kinase activity in single cells using translocation reporters. Nature protocols...whose cellular localization serves as a proxy measurement of kinase activity. The key advantage of KTRs...nucleus, while FRET-based reporters can be used to measure kinase activity in specific subcellular compartments...

... useful tools because we can easily detect and measure the light they emit. Proteins that give off light...observed in the crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the underside of the...this new green glowing protein could be used to measure gene transcription, as well as to track protein...GFP’s function. His lab described the protein’s structure and also discovered many mutations that alter ...

... a dish that features that ingredient. We just had a cook-off last week, and the featured ingredient was...Harry Potter-style.The shared lunches create a culture of openness and trust that comes from caring about...ll dedicate a post to these competitions in the future, and hopefully include some of our favorite winning...

...values with each element. We also set out to make sure the page looked clean, professional, and modern....our website, or in social media, we wanted to make sure our friend matched our core branding. Blugene needs... our values of community and sharing.  One big feature upgrade? Blugene now has a full expression. You...Addgene's new search tool Read more about other features of Addgene's website Resources on Addgene.org...

...heart health. These are amazing advances that will surely save thousands if not millions of lives, but many...resources and promoting research for rare disease cures. Through their BeHEARD Award, the Rare Genomics ...better choose which genes and pathways to target in future small molecule screens. He plans to work with a...voice. Check back with Addgene often to learn of future rare disease research funding opportunities. References...

...That includes keeping fees as low as possible to ensure that our materials are affordable but enough to...community-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better job of... our core values. These accurately reflect our culture and outlook: Addgene increases productivity by...increase sharing in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes...

... first need to decide the best way to track or measure the process you’re interested in. Depending on ...examine, i.e. for an enzymatic reaction, you could measure consumption of a substrate OR production of a product...molecule biosensors Addgene has many sensors that measure the concentration of small molecules like arabinose...withstand a particular solute concentration or temperature? For further advice, see our posts on how to ...

...very painful FOP flare ups. Eventually, without a cure, AJ will begin losing mobility and not be able to...play like the other kids his age. A potential FOP cure But, there is HOPE. FOP is a genetic disease typically...solutions to treat rare diseases like FOP until a cure is available. Addgene and AJ's Avengers This year...knowledge of FOP, it’s biological causes, and potential cures. We encourage any researchers working on ACVR1 or...

...Rodríguez et al., 2011).  Genetics of SPARC Figure 1: SPARC and SPARC2 use the GAL4-UAS system along...sparse (S) labeling of the cell population. Figure 2: Dense (c), intermediate (d), and sparse (e) ...gene expression and clonal analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/...

... visualization. However, due to the nature of the size, structure, and other qualities of each tag class...individually isolate a protein, there must be a special feature about that protein that no other protein in the...for an extra clean up step of the tag – a handy feature. If you decide not to cleave the GST tag after/...

... lunch. There was something so special about her pure interest and curiosity to understand my work and...making of this photo!  Here, I was also given a mixture of support and independence that allowed me to ...on content strategy. I really believe that the culture at Addgene cultivates trust between coworkers and... more confident in myself, my abilities, and my future - something I didn’t expect from a co-op experience...