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Showing: 681 - 720 of 879 results

  1. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Articles with and without plasmids at Addgene To find out whether scientific articles associated with ...Addgene plasmids get more citations So what do we find? Lots more citations for the papers with plasmids...adjustment is done within publication years. What do we find once we do this adjustment? That papers with plasmids...

  2. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...strandedness) along with its action on the substrate (nick, bind, cleave, mixed). This information represents the...Considerations section on each enzyme page, you'll find a link to search Addgene's collection for plasmids...embedded in the References lists on every page you can find direct links to plasmids from the article if they're...

  3. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...have moderate to high immunogenicity, meaning they induce an immune response in the host. So why use a gamma-retrovirus...also has low immunogenicity, meaning it does not induce the same high immune response as retroviruses. ...immunogenicity, but some strains have been engineered to induce a lower inflammatory response within hosts. Relative...

  4. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...to the right indicates a higher signal, or upregulation, and a peak to the left indicates a lower signal...case for you, switching to a contour plot should indicate where the two populations diverge. The smear ...

  5. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...and/or opportunities) to modify the device for individual experiments. Manufacturers rarely provide the...for use, or they can modify and optimize the code, find and fix security-critical bugs, and contribute to...produced quickly with the printer, act here as a kind of "teleporter," since a part developed in Germany...

  6. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...nuclease. This can be determined by measuring the indel frequency at a target site. A specificity ratio ...the Addgene blog Read our CRISPR 101 blog posts Find more information about Cas proteins Download the...plasmids and resources page Read our CRISPR guide Find CRISPR plasmids for mammalian systems ...

  7. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...generates an action potential (Figure 1). This light-induced excitation can take place in a tissue culture dish...ChRgers and ChR2(H134R) both reach the brain, but individual neurons do not express as many copies of the ...available at Addgene Visit our viral vectors guide Find our chemogenetics and optogenetics science guides...

  8. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...do to combat this issue. First and foremost, be mindful of your extractions- if you need really clean RNA...blog Read blog posts related to plasmid protocols Find protocols for purifying DNA without a kit Resources...Addgene.org Check out our molecular biology protocols Find protocol videos on our website Read our Molecular...

  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ... provide detailed evidence of knowledge new to mankind. And you probably understand how much faster it...class. Even so, it’s quite easy (and common!) to be blind to the cognitive load you’re placing on your audience... as pausing or turning off the slide deck (or reminding them to put their phones away), or maybe I was...

  10. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...middle of my presentation, lost in a sea of data, my mind went completely blank. After several seconds of ... classmates so they could understand it. When my mind went blank, my options were to stop talking until...removal service slams dumpsters outside the open windows in the middle of your talk. Technical difficulties...

  11. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...20th anniversary was about all the amazing years behind us, as much as it as about the amazing years in...item, and had a cumulative 120,000 citations of individual items in our repository, a tribute to how many...sharing their plasmids. It’s been a privilege to find and share what stories we can, and to hear the stories...

  12. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...contributed by guest blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, Stanford University...most of the results were positive, the analysis did find 263,000 more osteoporotic fractures. The overall.... To remedy this, Shah et al. provide a table indicating the sex of the most commonly used cell lines ...

  13. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...You can create an almost limitless number of DNA binding proteins with Cas9 guided by different promoters...large as natural regulatory networks. Addgene: What kinds of things could we do as genetic circuits become...  Thank you to Chris Voigt for speaking with us! Find the Plasmids: Plasmids for multi-input CRISPR/Cas9...

  14. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...Potential points where DNA errors can be introduced, indicated by exclamation marks. Error points include places... frame shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements. ...bioinformatics. Through their efforts, the QC team finds errors in 30% of deposited plasmids! We are committed...

  15. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...nature20149 Jiang Q, Meng X, Meng L, et al. Small indels induced by CRISPR/Cas9 in the 5’ region of microRNA... unexpectedly affect nearby genes (Figure 1A-C). Find CRISPR plasmids at Addgene! Knock-in: Introducing...

  16. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...from and unique single stranded DNA primers that bind upstream (5’) and downstream (3’) of the DNA region...polymerase is also stable at this temperature and can bind to the primers.  Extension- The temperature is then... can learn all about ddPCR for AAV titration and find helpful tips and tricks in our blog post “Droplet...

  17. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...editing!  CRISPR functions in use But exactly what kind of editing are people doing with all these CRISPR... modifier. We used these categories to see what kinds of CRISPR tools people were depositing and requesting... and the longevity of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is...

  18. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...appropriate? If you already have an end product in mind, steps 1 and 2 will generally be straightforward...links to a CRISPR forum where you may be able to find information regarding your system of choice. The...driving Cas9 expression can be constitutive or inducible. U6 promoter is typically used for gRNA. May contain...

  19. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...their diverse utility, DNA transposons have been hindered by their limited programmability: depending on... crRNA, QCascade complexes survey the genome and bind target DNA sequences using RNA-DNA base pairing....Resources on Addgene.org Visit the CRISPR guide Find CRISPR plasmids for bacteria ...

  20. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...these frustrating and often months-long quests to find the right AAV for your system or to successfully...improve our Addgene Data Hub, 76% of respondents indicated they wanted to see more negative data (Figure ... e-book Additional Resources on the Addgene Blog Find and Share AAV Data with Addgene's New AAV Data HubCelebrate...

  21. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...networking at the time, but that means it was the best kind of networking! Since nearly every talk I’ve given...their interest. I won’t claim that I blew their minds, but I think many of the students learned something...Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources ...

  22. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...techniques Find tips on gRNA design Resources on Addgene.org Browse all CRISPR plasmids Find validated... and stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus...

  23. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...supernatant containing antibodies. The antibodies bind the immobilized ligands and can be eluted once the...are incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet is used to separate...Recombinant antibodies should be validated to ensure they bind their antigens specifically. Possible applications...

  24. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...are also available (Weigel and Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing an ...background growth of non-transformants and make finding true transformants more difficult. Don’t leave...arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource page Deposit...

  25. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ..., they fail to account for the effects of Cas9-induced dsDNA breaks. Are off-target cutting patterns in...hypersensitivity, or are in an enhancer, transcription factor binding site, or a coding region. Targeting these sites...Check out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries currently available...

  26. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...visually select cells in a microscopy image for individual analysis. A shuttle vector designed for this ...plasmids are those that can replicate in multiple kinds of bacteria, including many natural plasmids. By... same molecular language as the tree outside my window! Whatever you use them for, shuttle vectors can...

  27. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...that! I organized my lab data so well that I can find the results of any experiment I’ve run in under ...format regardless of whether you’re in academia, industry, or nonprofits. Like any other letter, they have...Our Management for Scientists Series Get Tips on Finding Your Perfect Job after Graduation Additional Resources...

  28. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...for a bacterial infection. While this post will, indeed, focus on bacteria, it is important to note that...resistance genes from it’s environment (from DNA left behind after another bacterium died for instance). These...Are Used in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use Our Microbiology Pages...

  29. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...plasmids for use in CRISPR and Tol2 transposase induced transgenesis.  Xenopus Xenopus, or frogs, are one...Addgene.org: Browse our plasmid collection page Find more plasmids and resources for plant resources ...Collection Page Read our molecular biology reference Find plasmids for your research ...

  30. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...to email me at [email protected]." You can find his original blog post below! --Ed.  Eight years...fundamental to development. I'm interested in how cells find their way to the correct positions. What is the... fit into a department and bring in grants. What kind of colleague will you be? Do you understand what...

  31. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...hydrogen bonds and a base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined ...conditions to be screened per plate. The results indicated that the optimal buffer for Taq DNA Ligase contains...DNA ligase I completely encircles and partially unwinds nicked DNA." nature 432.7016 (2004): 473. PubMed...

  32. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...it is also important to know the brightness of individual fluorophores. Table 1 describes the brightness... conjugated to. Since CD45 is a common marker, finding an antibody already conjugated to my fluorophore... In this post, you learned about the principles behind fluorophores and their colors for used in flow ...

  33. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ... for bench scientists everywhere. In it, you can find an introduction to viral vectors and their elements... in a clean and easy-to-navigate design. You can find it, along with other eBooks in our collection, on...

  35. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...and more. It's this diverse group of talented individuals that makes Addgene — well, Addgene. We're so ...delicious food, or playing one of the giant games or indoor mini-golf course.    While the main purpose of ...

  36. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...partnered with distributors in China, Korea, Japan, India, and Brazil.  We've also created a robust support...us the honor of being part of yours. We hope you find our services helpful in your scientific pursuits...

  37. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...synthetic biology can be difficult to find. I hope that many people will find this guide useful as they start...

  38. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...under this application process since May 2020. We find this process takes 4-6 weeks on average. Therefore...licenses is available through the US Bureau of Industry and Security. If you have any questions about ...

  39. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...interested in making your own DNA ladder? You can find the Tan Lab’s DNA ladder prep protocol here and ...Frugal Scientist? Save Money with the Baby Gel Box Find 5 Smart Phone Apps for Lab Life Learn About the ...

  40. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ...resource for scientists around the world. With that in mind, we are very excited to debut our brand new Addgene...goal of this page is to make it easier for you to find content relevant to your research and career goals...
Showing: 681 - 720 of 879 results