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Showing: 851 - 900 of 932 results

  1. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...within an organization, there can be variance. At Addgene, our content team, where writing is a core skill...updating this post, I took an informal poll of Addgene hiring managers. All of them stated that a good... in May 2024.     Additional Resources on the Addgene Blog Learn to Develop Your Professional Network...

  2. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...Resume Writing Resources on Addgene.org Check Out Career Opportunities at Addgene ...it's impact on human health.     Resources on the Addgene Blog Learn How to Experiment with New Careers ...

  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...In many experiments you’ll be working with a heterogeneous cell population, for example from a processed...voltage in the cytometer’s software in order to generate signals in a reasonable range. If colleagues in...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Flow Cytometry  Antibodies...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ... care about these details, but does follow the general structure and flow of the entire session. I myself... then see how many of them have requested from Addgene. It is always gratifying to see all the hands go...Herminia Ibarra and Kent Lineback Resources on the Addgene Blog Check Out Our Networking for Scientists Blog...

  6. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... scientists Resources on Addgene.org Learn about open positions at Addgene Check out our career videos...that loves you back. Additional resources on the Addgene blog Check out our transferable skills guide Learn...

  7. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...Parametric tests  Statistical tests fall into two general categories: parametric and non-parametric. In this...returning false positives/negatives, but they all generally yield a similar result and are appropriate to ...common parametric comparison tests along with some general statistics knowledge to have in your tool belt....

  8. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...PubMed PMID: 26186186.  Additional Resouces on the Addgene Blog Lightsheet Microscopy Choosing Your Fluorescent...Fluorescent Protein for Your Experiments Resources on Addgene.org Browse Our Fluorescent Protein Resources Find...Fluorescent Protein Backbones Find Plasmids for Optogenetics ...

  9. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...Addgene is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how...offer. Happy CRISPR-ing! More resources on the Addgene blog PRIME Editing CRISPR 101: Cas9 vs the Other...

  10. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...this year! All winners receive an Addgene care package with Addgene mugs and some delicious hot chocolate...

  11. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...EikeMahlandt @AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM #DeckTheLab pic.twitter.com...holiday season. Santa frog is coming to town. @Addgene @UMich_MCDB #christmastaco #frogsnowflake #xenopus...

  12. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...Human Cell Differentiation Molecules group Addgene Data Hub Any (see notes) Any Data repository...repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base  Any IBEX...

  13. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...Posts Last month there was plenty of excitement at Addgene. Our new viral service has begun to pick up steam... Lego Movie). October was no less busy for the Addgene blog which once again had record readership with...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...resources on the Addgene blog Technical Design of a Western Blot   More resources on addgene.org ...spend optimizing it. Blots for quantification generally require more optimization than blots used to confirm...

  15. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    .../10.1073/pnas.2018488117 More resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right Isotype...

  16. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers...journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped...

  17. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ... If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty ... are always looking to improve upon existing genetically-encoded probes by making them brighter, more ...

  18. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...controlling the amount of cutting and mitigating noise generated by off target digestion. After MNase digestion...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...

  19. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...besides structure and isotype determination, don’t generally affect antibody applications like ELISAs or Western...DOI:10.1126/sciadv.abl9171 More resources on the Addgene blog Antibodies 101: Introduction to AntibodiesAntibodies...

  20. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...which problems can be addressed and new ideas generated. Furthermore, this casual communication reflects...start something new! Additional resources on the Addgene blog Read our blog post about external collaborations...

  21. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...their work and their interest in science more generally to people outside of academia. Here are my recommendations...programs at both MIT and Boston University. At Addgene we're committed to scientific sharing – both your...

  22. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...video depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps; and an essay on...sport-related concussion.   More resources on the Addgene blog Summer SciComm Series: Cognitive LoadSummer...

  23. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...those ideas into commercial products and next generation companies.  We launched in the early spring of...Additional Resources Find more career posts on the Addgene blog Read our recent post on Synbio start up Revbio...

  24. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...score (Mattei et.al., 2022).    Figure 1: Generation of immunogenicity scores by predictive algorithms...and current Scientist, DNA Sequencing and QC at Addgene.  References and resources References Mosch, ...

  25. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... that while this post is intended to provide a general overview of CRISPR components, new Cas9 variants...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...

  26. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...TagRFP to give a brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will ...set of proteins the green and red proteins are generally the most detectable and so should be used to tag...

  27. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...studying up before you begin the hiring process. Read Genevive Bjorn's "Ready, Set, Hire" on Nature Jobs for ...Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing, and translation of results...

  28. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...helpful changes are endless.    Fig. 2: Blugene moves reagents from left to right as they use them...documents/Western%20Blotting.pdf  More resources on Addgene’s blog A Guide to Getting Started in Undergrad ...

  29. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...stellar resume and cover letter can be found on the Addgene blog.   3. Personal statement brilliance In addition...co-founder of STEAMid.     Additional resources on the Addgene blog Check out other tools and resources for early...

  30. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...This is the fourth post in the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot... first meeting and get organized. Discuss your general areas of interest and what you want to cover during...

  31. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...Framework Learning to think like an immunologist, a geneticist, a physicist, etc., is an immense task (it takes...accessible to your audience.  More resources on the Addgene blog Intro to our Summer SciComm Series...

  33. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...to plan ahead.     Resources Resources on the Addgene blog The Basics of Western Blotting Find other ...out other Antibody 101 articles Resources on addgene.org Western Blot Protocol Western Blot Protocol Video...

  34. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...Twitter into a paper-finding-machine. There are four general types of accounts you can follow: Scientists: ...scientific literature. Additional resources on the Addgene blog Learn why scientists should be on Twitter...

  35. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...Kamens on BitsizeBio  Additional Resources on the Addgene Blog Read our "Management for Scientists" series... on What Makes a Good Mentor Resources on the Addgene Wesbite Find plasmids to Expedite Your Experiments...

  36. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...Resources and references Additional resources on the Addgene blog Mouse Modeling Part 1 Mouse Modeling Part ...Popular Model Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression...

  37. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...more people to quit the lab. Resources on the Addgene Blog Read Joanne Kamen's Blog Series on Mentoring...Browse All of Our Career Posts Resources on the Addgene.org Find Plasmids for Your Research Learn About ...

  38. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... based on using a thin plane (sheet) of light generated by sunlight to observe single gold particles with...).  These viral plasmids are available through Addgene.  The combination of LSFM with multiple tissue ...

  39. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ... conventional antibodies, chemical dyes, and genetically encoded fluorescent proteins (Gambarotto, et ....7554/elife.46249  Additional resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence...

  40. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter...Internet "trolls"   More resources on the Addgene blog:  Summer SciComm Series: Cognitive LoadIntro...

  41. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...initiation proteins to start their own replication. In general, these plasmids tend to be low copy number. Conversely.../doi.org/10.1093/emboj/20.24.7323 Resources at Addgene Read our blog post on the Origin of Replication...

  42. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ... a common source of conflict. For instance, at Addgene, there are two teams whose primary role is customer... Jan. 3, 2022.     Additional Resources on the Addgene blog Management for ScientistsTransferable Skills...

  43. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...equipment that you use in your current lab first. Generally, major equipment can be defined as equipment with...Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental Designs Consulting...

  44. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...fellowship is that you are often vetted for your general scientific expertise and then once selected, you...Twitter at @Steph_Guerra Additional resource on the Addgene blog: Browse our blog posts on science career ...

  45. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...isotypes found in mammals.      Subscribe to Addgene's YouTube channel for more helpful animations, useful...

  46. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ... complete collection of protocol videos on the Addgene YouTube page. ...

  47. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...plasmids, they don’t go to Wikipedia; they go to Addgene.”-Ryan Mehl, Professor, Oregon State University...

  48. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...experiment. Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors? Check ...

  50. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...an eye out there for a chance to win some cool Addgene swag.  And, of course, to all our depositors: thank...
Showing: 851 - 900 of 932 results