We narrowed to 888 results for: URE

...major benchmark in characterizing the 4D nucleome. Future modifications and applications Ma et al. developed...

...experiments, B3 and KD were shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as ...

...genetic screens employ various on/off switches to measure the function of a gene. For example, they eliminate...

...current work also proposes promising avenues for future engineering to shift the spectra and improve the...

...the line!) and to visit scientists in person to figure out ways we can make Addgene better and make scientists...

...transfer plasmids and five different serotypes. Not sure what to request? Addgene's Data Hub can help, with...

...galaxy. TL;DR: Many roads need scientists open for adventure. Many thanks to our guest writer, Pamela J. Hines...

...fluorescent proteins. She also showed that R-bodies can rupture E. coli membranes. Read the full post for potential...

...of scientific concepts than can be gained from lectures alone. Using plants as an experimental system ...

... experience from the scientific community gets captured,” he says. “It’s not just a reagent, it’s an annotated...

...Historically, electrical activity in the brain was measured with implanted electrodes. The Archon1 voltage...

...these fantastic educational resources and will be sure to keep our eyes open for any new technologies that...

...number of researchers, I had high hopes of securing tenure in a British or Irish University where I would ...

...Time-bound. It is a good idea to take larger goals (e.g. Figure out what I want to do after I get my PhD) and break...

...of the reporter mRNA is labeled by PCP-mCherry (Figure 2). The 3’ UTR also contains a CAAX sequence to...

...generating your own fly lines. In the meantime, be sure to browse the publication references and web links...

...and numeracy on perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547...

... should improve the specificity of influence measurements by reducing photostimulation of the axons and...

...fluorescence after cleavage by executioner caspases. Failure for cells to die or cells dying at the wrong time...

...produced 92% and 87% mCherry protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR...

...connections, in a powerful way. Using brain structure to understand brain function So far scientists...

... brain, and holds promise in the development of future therapies. Our popular plasmid encoding a Cre dependent...

...plasmid sharing has certainly taken us on many adventures...From moving office locations three times, to...

... time to choose your own protein purification adventure. You want to purify your favorite protein (YFP...

...cell, this system is clearly also robust enough to ensure high levels of gRNA cleavage.   Xie et. al constructed...

...and train the participants in skills useful for future mentoring relationships.   If you would like to...

...much time does it take to make a plasmid? Figure 2: Survey results from a Twitter poll run in 2023...

... understand what you’re communicating. While I’m sure that there are loads of ways psychologists define...

... of protein of interest the membrane.   Figure 1: Electron microscope images showing ultrastructural...

...then digested with the Type IIS enzyme, and the mixture is ligated following a heat inactivation step. ...

... transcriptional and translational termination features with loxP sites on either side (often called a...

...plasmids as well as transfer plasmids. This concept ensures safe handling and expression of these vectors when...

...cells in order to determine the specific molecular features that allow such “chemical rescue” in vivo. Engineering...

...item list and set some approximate timelines. Be sure that you keep your commitments, and if for some ...

...particularly for labs that already do mammalian cell culture. HEK-293T cells are the most common cells used ...

...multiple rounds of purification to get your protein as pure as possible? Vectors that offer different combinations...

...construct transiently care needs to be taken to ensure that the proper cell line is used; several common...

...manage a large number of files as part of the job. Structure of a cover letter Cover letters follow the same...