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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: ACE

Showing: 341 - 360 of 610 results

  1. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...co-founder of www.assaygenie.com. No matter what facet of academia you are in, grant writing can be the...clear that you are staying because it is the best place in the world for your research to rapidly progress...

  2. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...Google it...Or Yahoo! it, or Bing it, but, let’s face it, there’s a reason nearly 70% of all internet ...experiment, but a picture of a gel or a sequencing trace file can go a long way in helping us understand ...

  3. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...gene cassettes. Thse vectors are engineered to replace the drug resistantance genes in the bacteria thus...known antibotics. These might be used on hospital surfaces and in hand sanitizers.  There are many clever...

  4. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... scatters a great deal once it enters tissue, placement above the site of stimulation guarantees that ...brain tissue a short distance so ideal implant placement should be about 0.4-0.5mm above the site of your...

  5. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...teamwork, manage day to day operations within their workplace, and pursue leadership opportunities at the local...postdoc). I soon realized that many of the issues faced by postdocs and institutions are not local but are...

  6. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...conferences, but after receiving her degree she was faced with a similar conundrum – she had the skills, training...color changing flower project was inspired by a Facebook comment.  In our day job, we worked on plants ...

  8. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...is typically purified using methods like ultracentrifugation, while lentivirus supernatants are centrifuged...collection 2–4 days 5–10 days Purification Ultracentrifugation (AAV); centrifugation (lenti) Centrifugation...

  9. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...applications across a wide range of topics. He embraces open science, self-publishing directly on his .... R., Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore...

  10. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...track protein localization, cell structure, intracellular trafficking, and protein turnover rates. Additionally...overexpressed fluorescent protein fusions is to replace the genomic copy of your gene of interest with ...

  11. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...so many technologies currently available, you now face the challenge of trying to figure out what system...Scientific Inc., Origene, and NEB® are all good places to look for some helpful kits! Efficiency: Wouldn...

  12. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...comfortable with awkward silences but I’ve learned to embrace them after one ended with the person in front of...your manager for advice if you’re not sure how to face a particular situation. But keep in mind that a ...

  13. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ..., but those outside of your field can point out places where you gloss over essential details or omit ...avoid rushing out the door without your poster, place it by your keys a day in advance. Dress for the ...

  15. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...simply as it seems laid out above, there are many places where the PhD application cycle becomes much more...program will allow for you to have the time and space to delve into what matters to you. It can also be...

  16. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... has refined our use of the BLAST web browser interface to be as efficient as possible. If you find yourself...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program...

  17. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...individual plasmid orders, because when a PI or lab places an order, they are likely to order several plasmids...next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of...

  18. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... journal club? Where will the journal club take place? Preparing for the first meeting There are many ...important pieces before that first meeting takes place to ensure success. One of the most important is ...

  19. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...required for a mini amplification reaction to take place.  When the PCR is complete, a droplet reader extracts...contamination. Optimize your PCR For a good starting place on what PCR parameters to use, see Lock’s seminal...

  20. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...research uses ancestry groups rather than self-reported race, it highlights the myriad issues researchers need...between biology and ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens ...
Showing: 341 - 360 of 610 results