Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 898 results for: CAN

Showing: 341 - 360 of 898 results

  1. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ... Yet little explanation is given for how someone can or should demonstrate this quality.   I am often ...high quality. Their work involves coordination and can require technical mastery. Scientists need to be ...hire and fire to make sure the team is the best it can be. Managers must ensure that their teams are working...operating in a different role?  I think the secret sauce can be summed up in one word – initiative.  Initiative... listed a few of my favorites. Additionally, you can find more suggested resources on this topic and get...Reads: On Leadership (Articles on Leadership, also can be purchased individually) ...

  2. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...although sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test ... pegRNAs whose 3′ extension begins with a C base can cause unwanted base pairing (left), while pegRNAs... What to edit The sequence you choose to install can influence the success of your prime edit. If possible...synthesized strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019). Figure...left), but “bubbles” of 3 or more mismatched bases can often evade MMR (right). Created with BioRender.com...PE3/PE5 approach. Figure 7: Nicking sgRNAs can be designed to target edited DNA, so that nicking...scaffold sequence (orange) and the genomic target can increase the odds of incorrect editing outcomes, ...

  3. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...overhangs, stat? GetSet can do that for you, complete with predicted fidelity. You can specify number of overhangs...highest-fidelity overhang set it can find for those parameters. For even more specificity, you can exclude specific sequences...overhangs with a high potential for mismatches, so you can redesign individual pieces as needed to improve fidelity...rather than pre-designed sets, so identical searches can return multiple answers. You might want to take advantage...fusion sites to split it into multiple fragments. You can define the number of fragments, and the “search windows...constricted to a specific number of fragments, you can also ask it to give the maximum or minimum number...52 fragments may be near the upper limit of what can be accomplished with Golden Gate Assembly—even when...

  4. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...experiments? While codon preferences can be very useful for organisms, they can be problematic for researchers...organisms - organisms have codon usage bias. You can find many codon tables showing which codons encode...constraints that lead to these preferences and what can we do about them? Read on to find out!   Why do organisms...conditions. With varied codon usage, an organism can change which proteins are highly expressed and which...instance, it may not express at all in E. coli (you can find a variety of databases showing various organisms...sounds. Even for relatively short peptides, there can be many possible ways to encode them and what constitutes...advantage of producing additional tRNAs is that you can use the same expression system for many different...

  5. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...The analysis can be sorted by any of the parameters displayed in the summary table. You can also use your...analysis formats: sample by sample analysis, which can compare up to five editing experiments at a time,...simultaneously.  The current version of ICE software can analyze indels that result from single or multiplex...red exclamation point in front of that sample. You can hover over the yellow or red circles to gather details...  The higher the R2 value, the more confident you can be in the ICE score. KO Score - Represents the proportion...to search for a particular sequence or name. You can perform more in-depth analyses on each sample by ...about the indel profile of the edited sample. You can also download the entire analysis as a ZIP file by...

  6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...two direct repeats which can be used for marker removal so that the marker can be reused to introduce multiple...present in bacteria. They consist of a toxin which can affect a variety of cellular processes and an antitoxin...by methanol. Once linearized, the delivery vector can be integrated into the genome to introduce the genetic... in the chromosome. The more stable endonuclease can then cut the chromosome leading to cell death. Antisense...resulting in cell survival. Plasmid addiction systems can be great additions to constructs that are used to...Kaper used the hok/sok system to create strains that can stably express antigens in sufficient quantities ...to construct a stable synthetic gene circuit that can be used over prolonged periods. Using a plasmid carrying...

  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...region or nucleus. Just optimizing AAV expression can take months (or longer), yet the process is often...detailed in the primary literature, and as a result can leave scientists under-informed with respect to how...cell types used for packaging viral particles (6), can influence vector performance. Open science initiatives...especially for AAV data, that may not be the case. We can all acknowledge that negative data is something every...getting this information out into the public where it can be utilized by others. Addgene’s AAV Data Hub is ...evaluate how small changes in vector administration can influence AAV expression properties.  By providing...generally captured in the literature, scientists can use the Data Hub to compare different methods and...

  8. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... that you can do now is diversify. Read on for an overview of some of the resources you can use to better...Conferences One of the single most important things you can do to plan for your career is to expose yourself ...homework and know who is going to be there, so that you can tailor your CV accordingly. Make it stand out with...you’ve identified an appropriate job opening, you can send your contact an email with the relevant info... position he or she originally called about, you can keep in touch and reach back out when you are looking...should have a well-groomed LinkedIn page. Here you can search for jobs, post your CV, and, most importantly...who they know all in one place! This information can be critical to a focused and effective job search...

  9. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...visualization for big biology. GPS for the genome We can think of the human genome as a map with three coordinates...addresses in the genome. Let’s now examine how we can locate powerful stretches of DNA known as enhancers...Through the marvel of DNA compaction, an enhancer can increase the expression of a gene that is millions...are bound to modified proteins called histones. We can detect modified histones with “GPS for proteins,”...tri-methylated histones, functions as an enhancer. We can therefore denote enhancers as follows: (mono_methylation... and conclude with a targeted CRISPR screen that can disrupt these enhancers. A real-world experiment ...H3K4me3 The following video demonstrates how anyone can find enhancers with Quilt. Seek and destroy enhancers...

  10. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...yours and your team's—can lead to frustration. Clinging to technical tasksDelegation can feel unnatural for...contemplating the leap into management, the journey can be as exciting as it is challenging. How do you... you need to know.   Figure 1: Management can seem a mystery at first! Image used under CC.  ...hands-on work. The temptation to "do it yourself" can result in burnout and underutilized team potential...connecting with mentors who have made similar transitions can provide you with insights, lessons from their experiences... Myers-Briggs Type Indicator or DISC assessments can help you navigate team dynamics effectively and foster... The Making of a Manager by Julie Zhuo Radical Candor by Kim Scott Courses: Leadership programs on...

  11. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...Gal4/UAS system, and you can read more about them in review articles (2–4). You can also take a look at my...expressed when Gal4 is expressed. Gal4 expression can be regulated by any chosen promoter, and Bloomington...expressing Gal4 in your tissue type of interest. You can quickly mate your UAS-YFG line with a variety of ... next blog post). For example, the hedgehog gene can be overexpressed in several cell types by using different...Controlling multiple genes with Gal4/UAS system One can also use Gal4 to drive expression of multiple genes...’s not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but...

  12. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...metabolism of leaves can be changed by light and temperature and leaf autofluorescence can interfere with microscopy...that replicate current knowledge, but the sensor can also kinetically outperform current methods. Unger...nucleotide polymorphisms present in the human population can affect drug and dose response. Selective serotonin...Unger ultimately hopes that medical professionals can genotype the patient and rapidly test the variants...promoters responsive to water or nutrient levels can help him determine whether the artificial leaf behaves...labelled with a constitutively fluorescent reporter can also be used to track how one population responds...Mitja Remus-Emsermann’s lab at the University of Canterbury where he studies how bacteria interact with each...

  13. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...grants and projects that might help your work Tenure can depend on your national recognition and the more ...academia is all driven by personal connections What can you do to get the most from the time and money you...conference. Large conferences have many opportunities but can be overwhelming as far as meeting new people. At ... want to introduce yourself and what you do. You can probably use some scientific terms when socializing... that already). Start with, “Is this seat taken? Can I join you?” Next talk about the food and then move...up to someone much more senior than you, but you can safely seek out scientists your own age. Some will...LinkedIn users notice this sort of connection. You can also see and offer congratulations on job changes...

  14. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...Figure 1). Antibodies can be broken up into smaller parts, the variable regions can be altered for specific...If you think functionality can be ramped up even more, the best mutant can then be used as the starting...delivering other treatments, their efficacy can be significantly increased through structural and functional...specific functions, the constant regions can be altered for different isotypes, and so much more. How you...low-fidelity polymerase, like Taq. Other PCR conditions can be changed to further lower fidelity, like changing... and the dNTP ratio. The resulting random errors can give you new and unexpected sequences. Degenerate...mutate whole antibody sequences, degenerate primers can be designed to selectively mutate specific regions...

  15. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... determines which tissues and cells an AAV can infect) can influence an AAV’s value greatly. Applying ...have different tropisms but, as you can see, AAVs of each serotype can transduce multiple tissues. So how...transducing different brain regions. Although AAV2 can transduce many tissue types, it has low expression... It has been demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move... If axonal transport is not desirable, transport can be reduced by injecting a smaller dose of AAV and... than other viruses, multiple freeze thaw cycles can still reduce AAV titer. For long term storage, make... begins. ScAAV avoid this rate limiting step and can immediately undergo replication and transcription...

  16. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...which level of purity. Based on these premises we can classify a plasmid preparation in 3 different ways...electrocompetent cells is minimal (picogram range). It can be extracted from small bacterial cultures (i.e. ...prokaryotes or eukaryotes into a synthetic vector that can have a plasmid or viral origin. The type of cloning... extraction. Restriction enzyme based approaches can require several hundreds of nanograms or micrograms...you want to utilize your plasmid DNA, so that you can decide what will be the optimal type of plasmid preparation...

  17. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...Career and skills development can happen anywhere, whether you realize it or not. In the lab, you could...two projects: one “safe” project that you know you can get a publication with and one “shoot for the moon...you don’t think you’re qualified. The worst that can happen is they say no, which is infinitely better...Senior Software Engineer “Searching for a new job can be an imposing endeavor.  Break the process down ...Get the toughest steps out of the way early if you can.” - Eddy Page, Digital Media Production Specialist...Kamens, Executive Director Career exploration “You can't wait for opportunities to fall in your lap. Sometimes...

  18. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ... – Get your voice out there Here’s one thing you can do today: Sign up for a Twitter account. Check out...in local museums, zoos or botanical gardens. You can also work or volunteer for a science communication...you don’t have time to maintain your own blog, you can probably write for your institute’s blog. The Wellcome...Use your unique talent to communicate science. You can draw live sketches at conferences like @ATJCagan ... by taking a media or journalism course. Courses can help you gain work experience and build your network... expect them to be the “be-all-and-end-all”. You can, for example, get an MSc Science Communication or...involve the public in your research projects, you can set up a citizen science project. Answering the big...

  19. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...constructs can be achieved by fluorescence-activated cell sorting, and stable gRNA expression can be achieved... But other enzymatic fusions, such as dCas9-TET, can, like the iron ball-and-chain that they resemble,...site is not as straightforward as expected. TET1 can have many effects independent of dCas9 targeting,...ve got some good news: complete DNA demethylation can be achieved by dCas9 alone, without TET (or any demethylase...demethylase) fusion. This may seem counterintuitive: how can a DNA-binding protein with no enzymatic activity ...DNA demethylation! With optimization, this method can produce nearly 100% demethylation without the confounds...block that unmethylated CpG so that nothing else can interact with it. Remember, the goal of this experiment...

  20. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ...hybridoma cell lines can produce more than one mAb (Bradbury et al., 2018). Hybridomas can lose gene expression...into a fully functional antibody. These antibodies can be used just as you would use antibodies made from...purified antibodies from a patient’s blood), scientists can use mass spectrometry to determine the amino acid...from an established hybridoma line, the antibody can be converted into recombinant form through DNA sequencing...2010; Andrews et al., 2019). Finally, scientists can perform the entire process of antibody selection ...been cloned into an expression plasmid, the plasmid can be introduced into host cells, such as bacterial,...engineer them. Given the ease with which scientists can now improve genetically-encoded research tools, it...
Showing: 341 - 360 of 898 results