Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 573 results for: CHI;

Showing: 341 - 360 of 573 results

  1. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...retrievable, searchable and traceable. However, switching from paper to an electronic lab notebook requires...within your lab? In every lab, there are certain hierarchies and restrictions to data access. If these are...

  2. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...biotin, avidin. Avidin was aptly named for the chicken, “Avian”, egg it was purified from. Snell later...Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...

  3. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...or difficult to create this list. Start BEFORE searching for a faculty position. This is important to ensure...seem, you should consider it if it will help you achieve your research goals, even if that means saying ...

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...for mammalian expression and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral...Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Although there are different ways to ultimately achieve success, we find it is usually faster and easier...large number of different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...need! How does it work? There are several ways to achieve multiplex IF. One of the most common approaches...the sample, either by chemical treatment or photobleaching or by stripping out the antibodies, then start...

  9. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...alternative sequencing approaches have been developed. Cas ChIP-Seq, where only DNA sites bound by Cas are sequenced...for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding sites within...

  10. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...rural health clinic in Ghana. My role involved teaching the nursing students and community health workers... world. The research project was hard; I was researching the epidemiology of breast cancer and I loved...

  11. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ... target. There are a multitude of options for achieving success in a science policy career with many opportunities...organizations, working in student government, and teaching and mentoring others (see more in Figure 2). Starting...

  13. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... mammalian cells. Type IV Type IV is the middle child (like me!) of CRISPR types — weird and poorly understood...involved in plasmid competition or can hijack machinery from other CRISPR systems to carry out various...

  14. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...string of letters is a part of the word you’re searching for. Antibodies may recognize a linearized sequence...a slide, or whole organs. Because the tissue architecture is preserved, this technique allows you to not...

  15. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...Cofsky et al., 2022; Osuka et al., 2018). Each matching DNA:RNA pair gives just enough stability for the...Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization...

  16. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... inhibitor with a degradation tag (LVA tag) to achieve specific point mutations in E. coli. gRNAs are ...plasmids from this paper at Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for ...

  18. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...versatile, but it requires careful optimization to achieve the best efficiency. The type of edit, genomic ...Liu lab contributed an improved prime editing “architecture” that can be combined with any of the PE2-PE5...

  19. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...With both siRNA and shRNA, it can be difficult to achieve sufficient knockdown to enable robust assessment...S., Wright, A. K. A., Sousa, J., Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P. (2019). Validation...
Showing: 341 - 360 of 573 results