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  3. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...transgene free individual. For example, with CRISPR-Cas9 gene editing, transformation may be identified by...CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation CRISPR 101: ...

  4. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...of a hairpin structure within your primer. In our case, we will add TAAGCA, resulting in a final Forward...to the sequence to be amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying...

  5. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...non-specific staining can be a challenge in the best of cases. But it can be particularly challenging when your...primary antibody that has your reporter (in this case a fluorophore) directly attached to it. (B) Another...

  6. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out...protein functionality (Arribere, et al). In rare cases, tags can also affect protein localization and solubility...

  7. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... common event. What are exosome AAVs? In 2012, Casey Maguire and colleagues from Massachusetts General...28082074. PubMed Central PMCID: PMC5368844.  Maguire, Casey A., et al. "Microvesicle-associated AAV vector as...

  9. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ... strategic knowledge to solve a clinical disease case. As a teacher, you explain the game rules and then...face each other on a single chosen clinical disease case. Then, each team received a whole set of cards (...

  10. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells,... delivering receptor constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are...

  11. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...computer modeling and predictive algorithms to forecast the drug epitopes likely to be presented by T ...between 2D assays and in vivo animal models. In these cases, they are designed to mimic the human lymphatic ...

  12. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...we all shared a passion for DIY Biology and, on occasion, would meet in the same space where BosLab exists...journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking...

  13. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ... start of the CRISPR craze, but he says, “In the case of CRISPR, the hype was completely justified… it...the audio engineer and producer for the Addgene Podcast.       Resources at Addgene Find CRISPR resources...

  14. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...of having all the pages in a Curricula Vitae to showcase your publications, academic performances, and ...related to the job you are applying for, in which case talk about your research, as an understanding of...

  15. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...should be interesting; however, this should (in most cases) not be the driving factor. The following questions...head for a career in pharma or biotech, this is one case where the topic of your research can make your transition...

  16. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...

  17. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...validate your antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite ...abundance transcription factor or cofactor. In such cases, the gentler enzymatic digestion can preserve more...

  18. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...photons or odorants, to elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled...then induce or inhibit intracellular signaling cascades as a response to the extracellular stimuli. Ligand...

  19. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...following exposure to a brief heat shock. In the case of Hsp70, the heat shock releases heat shock factor...depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans...
Showing: 341 - 360 of 600 results