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We narrowed to 662 results for: Clu

Showing: 341 - 360 of 662 results
  1. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and...mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes both polyadenylation...
  2. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...quantification tend to be overestimates since they can include free p24 and defective viral particles. Direct ...infectious virus in a prep as the measurement will include defective particles. Studies have found that values...
  3. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...biologists working together on a variety of projects including the OpenWorm project. The main goal of OpenWorm... track them. We have a number of microscopes, including an automated microscope but we need to develop...
  4. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...conducting literature reviews and having weekly journal clubs outside of work. Our scientific training was put...we had an idea, knew how to build it, but had no clue how to run a business. SynBio Axlr8r thought color-changing...
  5. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...AAV6 is reported to have broad tissue tropism, including skeletal and cardiac muscle, airway epithelial...be expressed Cre-dependently to allow tracing exclusively from genetically determined cells. This version...
  6. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...website and also for offline use via GitHub (which includes prediction in batch mode). To predict the best...passion for computational biology. His expertise includes applying machine learning techniques to make sense...
  7. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...compensation between each other. We can therefore conclude that this population is double-positive for both...that there is no issue with compensation here. Conclusion Compensating between your fluorescent markers...
  8. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...standard quality control assays for AAVs. These steps include measuring viral titer and assessing purity. But...also conducting more rigorous quality control, including next generation sequencing (NGS) of the viral ...
  9. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...introduce yourself and what you do. You might want to include your university and your advisor’s name for some... uploading your work to a preprint server (and include this information on the poster) so your visitors...
  10. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...marker like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology arms. When we...recombination using a repair template. These templates can include loxP sites to create conditional floxed alleles...
  11. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...in the screenshot below). Currently, the page includes interactive maps for the Ras, mTor, and p53 pathways...from the research community, will soon expand to include several other important pathways. We invite feedback...
  12. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...are needed to optimize yield. You might want to include an extra wash step or use a different strain. For.... Look out for carbohydrates Certain strains, including HB101 and derivatives, release large amounts of...
  13. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...mammalian production system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese ...specifically. Possible applications for validation include western blot, immunohistochemistry, and immunocytochemistry...
  14. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...and its RNA directed binding site. These methods include the fusion of an activation domain such as VP16... or tracrRNA sequences can also be modified to include RNA domains that recruit additional transcription...
  15. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...their production requires animal sacrifice and precludes genetic engineering of a tag or fusion with a ...IgG subclasses, so that secondary antibodies exclusively recognize only one primary antibody. This process...
Showing: 341 - 360 of 662 results