...and Benjamin Glick from SnapGene. SnapGene was created to meet a need. While there were software tools...the DNA molecules they were using. SnapGene was created to alleviate these problems through good software...control elements. Development of this tool required creating a database of common features, and devising rules...the beginning or end of the feature. With the increasing popularity of gene synthesis, many researchers...

...who are also students in AP biology classes Creating a podcast about genetics using dog breeds as an...structure, and intent to the messaging - while also creating space for people outside of the primary audience...your team?)  Defining your audience allows you to create common ground and understanding that you can use...Image courtesy of Ron Mader, via Flickr, under the Creative Commons license     ...

... of several SARS-CoV-2 variants coincide with increased vaccination efforts. So, how effective are the...Neutralization levels against spike pseudovirus decreases depending on the variant. The lowest levels of... assay to study SARS-CoV-2 spike variants. “We created a series of spike expressing plasmids which encode...different circulating variants. This allowed us to create pseudoviruses that represent lineages and variants...

...reminds us that, although fluorescent proteins are incredibly useful, chemical fluorophores can still be used...Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of Dr. Elke Mühlberger and Dr. Rachel...CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that can be used to quickly...barcode small libraries of CRISPR gRNAs to use for screening experiments. Get the full details on how to make...

...promoter, the gene of interest, and a polyA signal. Created with biorender.com. ITR structure  ITRs are symmetrical... and terminal resolution site (trs) are noted. Created with biorender.com. ITR modifications and types...containing transcription factor binding sites can increase transgene expression and transduction efficiency...terminal repeats reduces rAAV productivity but increases transgene expression. Sci Rep, 7(1), 5432. https...

...sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids to confirm they contain the expected...of plates will have select samples prepared and screened by NGS to spot check the samples, the rest will...are requested by scientists, will immediately be screened by our QC scientists to confirm the plasmids contain...By leveraging our core strengths of intensive screening and the use of high throughput platforms, Addgene...

...application of an electrical charge to cells, to increase cell membrane permeability and thus transformation...DNA can be accidentally packaged into the newly created phages. Transfer of this DNA to another cell is...Viruses (AAV). Lentiviral and AAV can be used to create both transient cell lines, where a gene of interest...Electroporation on the other hand uses electrical pulses to create transient pores in the cell membrane that genetic...

...RspWYL1 increases both targeted and collateral RNA degradation by RspCas13d. RspWYL1 also increased EsCas13d...Cas13d, both groups used very general bioinformatic screens that looked for a CRISPR repeat array near a putative... WYL-domain-containing accessory protein WYL1 increases RspCas13d and EsCas13d cleavage activity The majority...

...of Producing Monoclonal Antibodies, 1999). This creates an immortal cell population that will continue ...hybridomas for monoclonal antibody production. Image created with BioRender.com.   The antibody-producing... the genes from the hybridoma into a plasmid to create a recombinant antibody.     Figure 2: Changes...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

... preserving cell viability. Library delivery Screening libraries, large collections of biological independent...independent variables, are becoming increasingly popular tools with many applications. These libraries ...library cargo delivered are controllable. For many screening applications, it is essential to deliver a single...being explored as the delivery mechanism for increasingly popular nucleic acid-based vaccines. These vectors...

...product and development teams have worked hard in increasing and improving accessibility on our website.  ... In fact, one of our team goals in 2023 was to increase accessibility to our website through the use of...describe images in online spaces. Alt text is used by screen readers to provide visually impaired visitors with...ultracentrifugation. The viral supernatant migrates through increasingly dense layers until the macromolecule density...

...Welcome to our deep dive series, which aims to increase your understanding and technical proficiency with...temperature drops, single intensity should smoothly increase as the DNA amplicon transitions from single-stranded...finally, drop once more during a final temperature increase. If there is a single amplicon, there should only...c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma, 2019...can fit in a quick game of Connect Four! Image created in BioRender.   Passive Reference Dyes Whatever...

...it, it just reflects how useful the reagents we created are for the community. More than 100 labs worldwide...need for high-quality service, open data, and increased reproducibility of materials. In March 2022, we... supported by an NIH BRAIN Initiative. We also created a growing library of antibody educational resources...York, Calico Life Sciences. Most of all, we’re incredibly thankful for all the scientists who have joined...

...ions flow into the neuron and causes it to fire. Created with BioRender.com. To make channelrhodopsin-based...information about parental channelrhodopsins to create recombinant channelrhodopsin sequences. The model...fiber-optic cable allows blue light to reach the brain. Created with BioRender.com. In the first experiment...neurons in the VTA, this should propel the mice to increase their nose-poking behavior. Strong nose-poking...

...Voigt: The big promise is orthogonality. You can create an almost limitless number of DNA binding proteins...fundamental. We built a NOR gate because they are used to create any program you can imagine. The Apollo 11 mission...so that genetic designs of that scale could be created. Someone could take on an engineering project including...enough hardware to control hundreds of genes. We’ve created enough regulatory elements, CRISPRs, repressors...

...OpenWorm OpenWorm is an open source project that is creating the “first virtual organism in a computer.” This...and split cGAL systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system...reagent toolkit and plasmid assembly line for the creation of CRISPR targeting vectors that each contains...2016). Using SapTrap, this CRISPR plasmid can be created in a single tube. You can also find additional ...

...of Science Writers How to create a successful science blog  5) Get creative – Bring your knacks into ... live sketches at conferences like @ATJCagan or create agar art with colorful microbes? Try your hand ...Science communication is my way of living out my creative side. My newest, exciting endeavor is starting...

...suspected a data-driven approach might allow for an increase in the complexity. In 2018, they published a paper...conditions available in DAD tools While DAD made an incredibly complex reaction possible, NEB also optimized...Additionally, this assembly, which used only antibiotic screening for selection, failed under normal cycling protocols...Ultimately, data-driven Golden Gate Assembly greatly increases the potential complexity allowable by a favored...

...observe the effects. Luckily for us, CRISPR is an incredibly versatile system that can be tweaked to target...pairs long. Using a single guide RNA (sgRNA) to create a double-strand break works well because very small...ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for targeting thousands...092902 Liu Y, Cao Z, Wang Y, et al. Genome-wide screening for functional long noncoding RNAs in human cells...

...methods. While techniques such as  blue-white screening require X-gal, an expensive exogenously-added ...chromoproteins don’t require other substrates for screening. Thus chromoproteins can be used as markers in... used the amilCP and tsPurple chromoproteins to create negative selection markers for allelic exchange...transfer (FLIM-FRET) in living cells (Figure 4). They created ShadowR by modifying the surface of the chromoprotein...