We narrowed to 803 results for: Cre

... to screen for positive clones in most cases, and restriction enzyme digest can be used to screen plasmids...strand was degraded. Beta: Beta protects the ssDNA created by Exo and promotes its annealing to a complementary...recombination frequency between 0.1% to 1%, and can be increased to as high as 25-50% by designing oligos that ...recombination than dsDNA substrates, so you should screen more clones when using dsDNA. Have you used the...

...Each path to science policy is unique and this creates a rich and diverse workforce. Out of all the career...QUESTION. Science policy is the work you do to creatively connect seemingly disparate individuals and ideas...also happen at the state and local level. The big secret about science policy that I didn’t learn until ...bring researchers to Congress to advocate for increased science funding and other issues. Whether you ... field where you have no specific scientific credentials.  But before you even apply to that first science...

...my partner Mike and I split the work, shared the credit, and we both won accolades that would launch our..., the Senior Scientist who coordinates all kit creation and quality control efforts with depositors; .... We worked with Andrew, the team’s founder, to create a mission statement in the first few meetings and...becoming part of a team is a really good way of creating and strengthening those social bonds. So never...communication options. Addgene, along with an increasing number of academic labs, uses Slack constantly...

...be used in concert with in silico prediction to create a more comprehensive list of off-target editing...of DSBs throughout the genome. However, their decreased sensitivity means that the best option moving ...with a focus on the ways science can be used to create a more just and equitable society. Read more at.... Bell, Charles C., et al. "A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations...Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9...

...paths. Check out Joanne's Reddit AMA Here are some concrete things you can do to develop those transferable...Manage a project involving multiple scientists, create and stick to timelines Be a mentor in a formal ...If opportunities don't exist in your department, create them and show your leadership skills too. Teach...are always excellent Technical knowledge and creativity Become a “thought leader,” develop a technical...

... drug discovery by big pharma (i.e. HTRF and LanthaScreen) (Degorce et al., 2009), measuring intracellular...are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET... biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore...donors (Fig. 1). Scores of FP variants have been created, thereby enabling a broad spectrum of donor fluorescence...strategies. FRET experimental design lends itself to creativity and innovation, valuable commodities in any NIH...

... The MORF library is only one of 22 libraries, created from 14 deposits, that became available through...would be remiss not to mention that 2024 is an incredibly special year for Addgene: it marks our 20th anniversary...twenty years while seizing new opportunities to create even greater value for the scientific community...every Addgenie, thank you for twenty years of incredible success, and for believing, as we do, that sharing...

...including Andrea Radtke, PhD, and Ziv Yaniv, PhD, have created the IBEX Knowledge-Base, a community repository...from the Zenodo dataset.     Figure 2: Screenshot showing reagent entries with associated metadata...others in the IBEX community.   Figure 3: Screenshot showing recent topics in the IBEX discussion ...when designing the IBEX Knowledge-Base, seeking to create an iterative process that is constantly becoming...

...COVID-19 research? We decided to give it a try, by creating a service for COVID-19 researchers. So far, we...to researchers worldwide. “COVID-19 has spread incredibly fast and without any notion of national boundaries...-19. “Open scientific sharing is important for creating research synergies and accelerating the translational... but we believe that by coming together, we can create a significant impact on both clinical care and ...

...kit size, it may be tempting to try increasing your input to increase your output. But, exceeding a kit’...their relative qualities and quantities. Image created in BioRender.com. Transformation Grade DNA...that you are left with pure plasmid DNA. Image created in BioRender.com. For the clean-up stage, plasmid...kit.   Do you want eco-friendly options? It’s no secret that labs produce a lot of waste. If you are looking...

...within content and business.  Joining the Addgene crew!  This pattern was consistent: everyone I spoke ...One of the bigger projects I was tasked with was creating and sustaining a biweekly report of our social...coworkers and that trust then translated into this incredible experience.  Of course, I wasn’t presenting to... research on loads of different best practices, create an email strategy, and make the occasional TikTok...

... at eScience Labs, an educational company that creates hands-on science lab kits and digital curricula...writing is clear, Caitlin’s career plans weren’t concrete when she finished grad school at the University...their research from the bench to the classroom by creating the outline of a college level course based on...

... to honor the creativity, passion, and dedication to sharing epitomized by the creators of these plasmids...distribute our plasmids via Addgene. Given the increasing popularity of CRISPR in the plant community, ... to send these small tokens of thanks for the incredible impact our depositors have had on Addgene and...

... then degraded by the cell.4.) The transposase creates a double strand break in the DNA and allows the...transposase gains its name from the fact that it was recreated from a defective transposase found in fish. This...transposases. Improvements have been made since then by increasing the consensus between a greater number of extinct...primary cells. In 2009, Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity ...

...reminds us that, although fluorescent proteins are incredibly useful, chemical fluorophores can still be used...Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of Dr. Elke Mühlberger and Dr. Rachel...CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that can be used to quickly...barcode small libraries of CRISPR gRNAs to use for screening experiments. Get the full details on how to make...

...who are also students in AP biology classes Creating a podcast about genetics using dog breeds as an...structure, and intent to the messaging - while also creating space for people outside of the primary audience...your team?)  Defining your audience allows you to create common ground and understanding that you can use...Image courtesy of Ron Mader, via Flickr, under the Creative Commons license     ...

... of several SARS-CoV-2 variants coincide with increased vaccination efforts. So, how effective are the...Neutralization levels against spike pseudovirus decreases depending on the variant. The lowest levels of... assay to study SARS-CoV-2 spike variants. “We created a series of spike expressing plasmids which encode...different circulating variants. This allowed us to create pseudoviruses that represent lineages and variants...

.... “They took a dividing yeast and did a genetic screen to identify the factors that regulate cell division...Leuven in Belgium. “That’s where I not only got incredibly compelled by wet bench molecular research, but... over the years. “Some of the walls we’ve built create inefficiencies that ultimately make innovation ...science slow,” Vandenberghe says. “The continuous creativity that Addgene takes to find ways to disrupt these...

...and Benjamin Glick from SnapGene. SnapGene was created to meet a need. While there were software tools...the DNA molecules they were using. SnapGene was created to alleviate these problems through good software...control elements. Development of this tool required creating a database of common features, and devising rules...the beginning or end of the feature. With the increasing popularity of gene synthesis, many researchers...

...product and development teams have worked hard in increasing and improving accessibility on our website.  ... In fact, one of our team goals in 2023 was to increase accessibility to our website through the use of...describe images in online spaces. Alt text is used by screen readers to provide visually impaired visitors with...ultracentrifugation. The viral supernatant migrates through increasingly dense layers until the macromolecule density...