We narrowed to 364 results for: DUR
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TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....always have a few extra slides in their back pocket. During your practice rounds, encourage your listeners ...
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Site Directed Mutagenesis by PCR
TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m.... successfully anneal to the plasmid of interest during the PCR reaction. Ideally, your primers should ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....target sequence. Kaminski et al. next tested their procedure in HIV-1 infected T-cells to see if they could... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....important modulators of cell signaling pathways during embryonic development and cancer. But there is ... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific... -
Plasmids 101: Dimers and Multimers
TypeBlog PostPublishedJan. 10, 2023, 2:15 p.m....whole plasmid sequencing of our plasmid samples during our quality control process. Since MiSeq involves... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m.... ADH1 promoter pADH1m, which maintains activity during the late ethanol consumption phase of yeast culture... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive rates and... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....insertions were positive or negative selected for during the experiment (Sandoval-Villegas et al., 2021)... -
A History of Genome Engineering in Popular Culture
TypeBlog PostPublishedFeb. 25, 2020, 2:15 p.m.... sparked the imagination of most of the authors during this period. Genetic Engineering Era: Pre-CRISPR... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m.... do with the slight melting of the 5 ́-terminus during the reaction. This “peeling back” of the 5 ́-phosphorylated... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m.... organisms which I use to study gene regulation during developmental processes, CRISPR/Cas9 allows us ... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....photon is emitted. This step is called emission. During excitation, some of the photon's energy is lost... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....without the undesirable side products generated during chemical synthesis of these molecules. To make ... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m....evidence,” and to speak out in unprecedented force during the 2015 federal election. Even as we marched... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis." J Cell ... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....glycosylase inhibitor (UGI) components, which inhibit BER during cytosine-to-uracil editing, and instead introduced... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m.... plasmid information. Enter plasmid information During the remainder of the process, you will enter plasmid... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m...., it is much easier to genetically modify cells during the reprogramming process as they are propagated... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog PostPublishedSept. 10, 2020, 11:45 a.m.... Note: depending on which method you use, the procedure will vary slightly. Two Golden Gate options...