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We narrowed to 694 results for: EED

Showing: 341 - 360 of 694 results

  1. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...packaging capacity, AAV vectors were suitable for our needs for the following reasons: Safety: Since AAVs ...should be used to clean AAVs from workspaces. Speed: AAV transduction experiments are relatively quick...useful for a wide variety of experiments, care still needs to be taken when selecting which particular AAVs...

  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...DNA sequence encodes the proteins the transposon needs for transposition. Transposition protein: This is... transposons” since they encode the protein they need to move around, either a transposase or a reverse...transcriptase. Non-autonomous transposons, however, need another TE to express a transposase or reverse transcriptase...

  3. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...jump from one antigen presenting cell to another. Indeed, we now know that T cells are able to crawl more... injury in the acutely inflamed glomerulus." Proceedings of the National Academy of Sciences (2016): 201606253...cell subsets in the skin-draining lymph node." Proceedings of the National Academy of Sciences 113.4 (2016...

  4. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...the end, this results in an experiment that could need more than 8x107 successfully targeted cells. Depending...the analysis becomes easier, the number of cells needed is lower, and perhaps most important, targeting...good idea to keep a bioinformatician close if you need help putting your list together. Once you have your...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...should also be considered. If the light intensity needed for activation is too high, the photoactivation...photoswitching between two fluorescence wavelengths. Indeed, within the world of super resolution microscopy...UV-inducible green-to-red fluorescence conversion." Proceedings of the National Academy of Sciences of the United...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...grow haywire, access and create more vasculature to feed themselves, use the blood stream to commute to and...inflammatory responses can augment tumor growth. Indeed, lasting responses to viral infections have resulted... inhibit expression of co-stimulatory molecules needed for T cell effector function. Cancer cells also...

  7. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...as excitation wavelengths and emission profiles, need to be matched with the experimental setup and objectives...Resonance Energy Transfer (FRET) applications, the dyes need to have compatible excitation and emission profiles...witnessed remarkable advancements, driven by the need for more precise, stable, and versatile imaging ...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...we’ve included a glossary at the end if you ever need to double-check your vocabulary! A brief review ...double-strand breaks. The only things base editors really need from Cas9 are sequence specificity and the R-loop...efficient and precise editors. Most researchers agreed on the first part of the likely editing mechanism...

  9. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    Published
    Jan. 7, 2020, 1:55 p.m.
    ...would like you to meet...  Hmmm, they need a name. And we need your help in finding a good name for Blugene...

  10. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    Published
    Dec. 5, 2013, 3:27 p.m.
    ...should our new blog be any different? If you still need an extra push, there’s evidence that laughter really...’s a way to connect with other people. (We don't need evidence for that one, do we?) OK, so, now that ...

  12. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...though I suspect that our costume contests were indeed occurring earlier but that the photo evidence was...how seriously we take costume design. In case you need more convincing, I’ve got just the proof: Above...

  13. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...in interactive figures is a great step up, but I needed a way to show different aspects of the data simultaneously... R can further customize the dashboard to their needs. I hope you find the dashboard useful when analyzing...

  14. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...biologists alike. Now with over 600 articles, we needed a better way to organize and present the vast collection...outside of these five areas and you can find what you need by field (ex: microbiology, neuroscience), or explore...

  15. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...choosing the cargo, serotype, and prep size they need for their experiments — and we’ve been able to ship...continue to expand the collection to meet the diverse needs of our community and support a wide range of experimental...

  16. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...developing a strong graduate student community and the need for belonging in the scientific environment 3:09...graduate school? - The importance of criticism and feedback 15:38 - 17:06 : Post Grad Plans 17:07 - 18:29 ...

  18. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ... commitment to be responsive to the community’s needs and our commitment to top notch quality service ...as we grow and change to meet its ever-evolving needs. For now, we have many exciting things planned ...

  19. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...scientific community to speed COVID-19 research. With the COVID-19 pandemic, we saw the need to expand reagent...
Showing: 341 - 360 of 694 results