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We narrowed to 677 results for: Eng

Showing: 341 - 360 of 677 results

  1. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...Vincent's tweets.      Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  2. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...Vincent's tweets.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  3. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...You can try a few different lysing buffers and strengths. If that’s not working, consider using a subcellular...incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect... Different lysing buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular...

  4. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...details. "Understanding this structure may help us engineer around the current limitations of the Cas9 complex...said study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future,...

  5. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols...

  9. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-...transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann...

  10. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming...tools in your experiments.  Many thanks to Hongkui Zeng’s lab for providing this AAV vector! Additional ...

  11. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk...

  12. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  13. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... However these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania.     References...

  14. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...Navigating a fulfilling career in science can be challenging – is advice and guidance from only 2 people enough...doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them Is not threatened by...

  15. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...which helped researchers visualize norepinephrine (Feng et al., 2019). But this first generation of tools...blog post. References and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou...

  16. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    .... Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor plasmids Cytosine...-ME) is a methylation editing tool from Albert Cheng’s lab that enhances RNA-guided methylcytosine editing...

  18. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...trained to be good at learning new things—this is a strength you can draw on and demonstrate in your application...hiring manager. Learn to confidently describe your strengths in your resume and don't be shy about asking for...

  19. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ... energy and to choose institutions with allies engaged in training excellence to begin effecting change...in their labs. Dr. Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to...
Showing: 341 - 360 of 677 results