We narrowed to 383 results for: FLU

1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ...critical to stem cell survival. Knowing which genes influence the survival of our cell culture is the foundation...

2. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ...and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (...

3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ... signal amplification in gene expression and fluorescence imaging." Cell 159.3 (2014): 635-646. PubMed...

4. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...protein-DNA and protein-RNA interactions. The 1980s saw a flurry of discovery in the field of eukaryotic transcriptional...

5. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ... is received in the lab, although this number fluctuates throughout the year (see graph below). Addgene...

6. Viral Production at Addgene

   Type

   Blog Post

   Published

   Oct. 24, 2023, 1:15 p.m.

   ...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre....

7. QC Sequencing Technologies at Addgene

   Type

   Blog Post

   Published

   July 15, 2025, 1:15 p.m.

   ...electrophoresis. Capillary electrophoresis reads a fluorescent label on the terminating nucleotide and records...

8. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

   Type

   Blog Post

   Published

   July 8, 2025, 1:15 p.m.

   ...you want to fuse your protein to any tags or fluorescent markers? If you’re creating a multigene construct...

9. Plasmids 101: Shuttle Vectors

   Type

   Blog Post

   Published

   May 20, 2025, 1:15 p.m.

   ...instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters...

10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...launch of the redesigned Data Hub in 2022. It’s influenced the way we approach our viral vector educational...

11. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    Published

    June 20, 2024, 1:15 p.m.

    ... number of iterons encoded on a given plasmid influences how high the copy number needs to get before ...

12. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  Resources...

13. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    Published

    Nov. 3, 2016, 2:30 p.m.

    ...own work. Of course not all research has to be influenced by research in other disciplines. But there remains...

14. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    Published

    July 2, 2019, 12:51 p.m.

    ...The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to isolate differentiated...

15. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell imaging approaches. ...

16. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...mouse adenovirus in mouse; HIV1, West Nile, and Influenza in humans), LY6A lacks a defined role in viral...

17. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    Published

    Aug. 18, 2016, 2:30 p.m.

    ... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...

18. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ...vectors are the solution. Flex vectors expressing fluorescent cytoplasmic markers (e.g. eYFP) and synaptic ...

19. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...scientists have studied how different parts of MECP2 influence Rett Syndrome. Zoghbi’s lab found that a small...

20. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...several previously observed characteristics that influence gRNA off-target activity: 1) mismatches closer...