We narrowed to 924 results for: Gene

1. Behind the scenes: Addgene’s new search engine and more

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   Blog Post

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   Dec. 12, 2019, 2:22 p.m.

   ...scientific domain in general and of our data in particular and our deep understanding of Addgene’s customer’s needs...’t. Try out the new Addgene search engine! Additional resources from the Addgene blog Learn about other...other features on Addgene's website Find a step-by-step guide to using Addgene's new search Read to learn... that we needed to improve more than that: the general “findability” of the information on the site. Different...posts or reagent items.  The technology behind Addgene’s new search  Like millions of other websites, we...approach much more than if we were dealing with general purpose information. Below are a few of the strategies...

2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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   April 5, 2018, 12:42 p.m.

   ... Figure 1: In conventional gene targeting (A), you must first generate your desired mutation in mouse...mutations, the 8-10 month process of conventional gene targeting is lowered to about 2-3 months. But certain...example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018) suggest using a 50-80 bp sequence... mouse model generation easi-er! Generating mice before CRISPR The process of generating knock-in mice...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models...after editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair...template. Figure 2: Overview of ivTRT to generate an ssDNA repair template. Although effective...

3. Addgene’s Viral Service - Why Virus? Why Now?

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   March 23, 2017, 2:30 p.m.

   ...expand Addgene’s services. There was an overwhelmingly positive response to the option of Addgene providing...allows us to maintain Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing... capabilities to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service ...build a completely self-supporting service so that Addgene is not dependent on grants. Aiming to operate under...Sponsors and Supporters Page Thank you to all of our generous, sharing depositors. Over 3,000 laboratories are...

4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   Feb. 22, 2017, 3:30 p.m.

   ... orientation as the gene or use custom gene synthesis or site directed mutagenesis to remove the BbvCI...this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol will still...work if the orientation of the BbvCI site(s) of the gene are in the same orientation as those in the plasmid...Additional Resources on the Addgene Blog Use Site Directed Mutagenesis to Generate Specific Mutations in Your... pot saturation mutagenesis as a quick and easy technique that can be used to generate complex libraries...of this. Plasmid library generation prior to one pot saturation mutagenesis Several methods can be used...effects. One-pot saturation mutagenesis The one-pot saturation mutagenesis technique described in Wrenbeck...

5. Using Addgene's New Viral Service for Your Research

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   Nov. 17, 2016, 3:30 p.m.

   ...Versatile Tool for Gene Expression in Mammals Additional Resources on Addgene.org Overview of Viral ...pooled libraries. Whether you’d like to knockout a gene, replace it, modulate its expression, or perform...while rtTA can be used to activate expression of a gene under the control of the tet operator. AAV AAVs ...associated virus (AAV, primarily chemogenetics tools for now but with optogenetic tools coming soon). The viral...excited that Addgene is now offering virion preparations to the scientific community. Addgene's AAV preparations...posts on the blog and pages on the Addgene website. blog.addgene.org The blog provides tips for working...email us and we’ll get you writing!     www.addgene.org On the Addgene website you’ll find our updated viral...

6. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

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   May 12, 2017, 12:58 p.m.

   ...plasmids deposited at Addgene from the abstract page. Check out that beautiful Addgene “Find Plasmids” badge...LinkOut” and search for “Addgene”. Then check the “Link Icon” box next to the “Addgene Nonprofit plasmid repository...Manuscript Resources on Addgene.org Learn How to Order Plasmids from Addgene Check Out Our FAQ Pages ...badge to be taken directly to the plasmids on Addgene’s site (Side note: I always recommend you read the...plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create an account... hope this helps! Additional Resources on the Addgene Blog Get Tips on Keeping Up with the Literature... addgene...

7. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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   Nov. 3, 2020, 2:15 p.m.

   ...delivery enables minimally-invasive gene delivery and optogenetic excitation. Excitation of neurons deeper...the differences between chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors...available at Addgene Visit our viral vectors guide Find our chemogenetics and optogenetics science guides...resources on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about...Optogenetics is a neuroscience method that lets you fire neurons with the flick of a light switch. Neurons...allowing an influx of ions into the neuron which generates an action potential (Figure 1). This light-induced... to use invasive measures to perform in vivo optogenetic experiments. Intracranial injections of AAV are...

8. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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   Sept. 17, 2020, 1:15 p.m.

   ...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small...Zuckerberg Initiative for supporting Addgene by providing a generous grant. This support will enable us ...sent the Addgenies home.  At the same time, as Addgene started dealing with this new reality of working...resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure...science sharing as we can get. The effects of Addgene’s brief shut-down and the subsequent fluctuations...don’t have space in this blog to call out every Addgene Team because they’re all doing amazing work during...rose to the challenge was our Finance Team. With Addgene closed for almost two weeks and with labs worldwide...

9. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   Aug. 9, 2016, 2:30 p.m.

   ...which NANOG-related genes are critical to stem cell survival. Knowing which genes influence the survival...compaction, an enhancer can increase the expression of a gene that is millions of base pairs away. (For details...GPS for the genome” to compute coordinates for genetic elements like protein, RNA, and DNA (Table 1). ...family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...differentiate over time, we periodically use next-generation sequencing to measure the relative proportion...pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing your own CRISPR...Hypersensitive Sites and Intergenic CRISPRs Yours truly generated the DHS data set by starting with all of the DHS...

10. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    Jan. 15, 2015, 1:50 p.m.

    ...versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed in the...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition to the poster session was a first...attendant called them, "long, skinny things") and generally exuding a very-tired-but-very-excited attitude...happens): Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges...That so many scientists are tackling this type of genetic engineering is a testament to how amazing the downstream...from the custom software used to design complex genetic circuitry to the downstream output. Tuesday morning's...

11. London Calling - Addgene’s New Europe Office in London

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    June 10, 2014, 3:20 p.m.

    ...contacting Addgene can be found on our Contact page. You’ve called. You’ve emailed. And Addgene has been...Plasmid sharing in Europe: Addgene’s request statistics Since 2004, Addgene has shipped more than 75,000...into the Addgene repository. More than 4,500 plasmids have already been deposited at Addgene by over 250... your service: Addgene’s London office Just a few weeks ago I became the newest Addgene Outreach Scientist...UPDATE - As of January 2020, Addgene's Europe office no longer handles customer support inquiries. The...Cambridge, MA to be exact. Today, that changes. Today, Addgene launches a brand new Europe office located in London...poised and ready to answer your questions about Addgene, plasmids, and depositing (all while our US counterparts...

12. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

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    Jan. 8, 2014, 3:10 p.m.

    ...frequently requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was...expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome engineering methods improved...Addgene was founded 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids...biosensors, optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium...Top 10 plasmid technologies distributed through Addgene over our first 10 years. 10 most distributed plasmid...it's not actually a technology, but pSV-PGC1 was Addgene's first distributed plasmid and was created in the...repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid...

13. Addgene’s New Way to Help You Share Science

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    Blog Post

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    Nov. 14, 2013, 4:10 p.m.

    ...When I got the job of Outreach Scientist at Addgene, my first thought was “I get to talk to scientists... spend a lot of time telling scientists about Addgene’s repository, and explaining how to deposit and ... 1,600+ labs around the world!) that have kept Addgene at the forefront of life sciences research for ...from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and get practical...scientific sharing and the open-access movement. At Addgene, we believe that science gets better when everyone... to tell? Let us know by emailing us at blog@addgene.org or tell us on Twitter and Facebook. We’ll track...

14. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

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    Blog Post

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    Feb. 6, 2025, 2:15 p.m.

    ...international researcher who uses plasmids containing genes from rabies and vesicular stomatitis virus, you ... export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster...ordering plasmids, please feel free to email help@addgene.org. We’ll be happy to help! A quick note: While ...complex and may change suddenly. Please email help@addgene.org for the most-up-to-date information on shipping...

15. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

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    Blog Post

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    Feb. 6, 2018, 2 p.m.

    ...Projects in Wei Leong Chew's lab. Genome engineering, gene delivery methods, AAVs, and more. Chapter 7/Outro... in the areas of genome editing, immunology, and gene therapy.   Special thanks to our sound engineer/...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...Additional Resources from the Addgene Blog Visit our CRISPR featured topic page Check out our Hot Plasmids...synthetic biology researcher Tom Ellis Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology ... addgene podcast...

16. Using AAV for Neuronal Tracing

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    Aug. 9, 2018, 1:04 p.m.

    ...Hybrid Serotypes: Improved Vectors for Gene Delivery. Curr Gene Ther 5(3), 299–310. PubMed PMID: 15975007...vectors could also be powerful tools for the field of gene therapy if they can reach the affected part of the...anterograde and retrograde axonal transport. Hum Gene Ther 25, 705–720. PubMed PMID: 24694006. PubMed ...2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems...., and Nakahara, K. (2011). Local and retrograde gene transfer into primate neuronal pathways via adeno-associated...-associated viral vectors is serotype-dependent. Gene Ther 20(3), 348–352. PubMed PMID: 22418061. PubMed...vector serotypes 1, 2, 5, 7 and 8 in mouse brain. Hum Gene Ther 18, 195–206. PubMed PMID: 17343566. Tervo, ...

17. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    April 6, 2021, 12:30 p.m.

    ...sequence to confirm backbone elements.   Confirming the gene/insert by aligning to NCBI entry or using BLAST....available reference sequence or plasmids containing genes with many modifications. We look for point mutations...amino acid. We also confirm that the species of the gene matches the data associated with the plasmid.   ...Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum...for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for quality control...control for over ten years. Now, thanks to a generous donation by the Hirsh family, we are able to take...provide requesting scientists high quality data! Addgene's new in-house NGS process To start, the process...

18. Hot Plasmids and Viral Preps - January 2021

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    Jan. 5, 2021, 2:15 p.m.

    ...control expression of their genes of interest. While recombinases are powerful gene expression tools in and...CRISPR knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the ...blue-light inducible Cre for precise control of bacterial gene expression By Meghan Rego Recombinases are enzymes...precisely controlled through the use of optogenetics. Optogenetic tools use light to induce conformational...Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive Cre, Opto-Cre-Vvd, by splitting...from Biggiephage can eliminate competing mobile genetic elements. Image by Basem Al-Shayeb in the Doudna...unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion...

19. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    March 5, 2020, 2:15 p.m.

    ...data Do you use Addgene AAV tools and want to join the ranks of our growing group of Addgene AAV data sharing...treatment conditions like drugs (Chemogenetics) or light (Optogenetics) to activate receptors? If you’re... way to celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot in 2019 as... an open science platform for scientists using Addgene AAV viral preps to easily find and share useful...animal strain used). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over...sharing champions? If you’ve recently used Addgene AAV tools, we invite you to contribute your data to ... for sharing on the site. The entry form has 5 general question types and it takes approximately 15 minutes...

20. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

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    Nov. 14, 2016, 3:30 p.m.

    ...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...we know biological resource centers, including Addgene, positively affect scientific research. As Maria...Maria Castro of University of Michigan says, “Addgene has transformed the way in which we share plasmids...tool is so influential that it is requested from Addgene many times as the technology disseminates throughout...researchers who have at least one plasmid deposited at Addgene that has been distributed more than 100 times. ... honor for our powerhouse depositors who have generously shared their reagents freely with the research... on sharing because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's of investigators...