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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 738 results for: HA

Showing: 341 - 360 of 738 results

  1. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... (SB) transposon system is one such system which has been designed to perform gene transfer in vertebrates...defective transposase found in fish. This transposase has been discovered in the genomes of many different ...

  2. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...enables you to discuss a person's performance or what has been learned, it helps to plan effective next steps...about areas they need to improve in. If a manager has concerns about an employee and the employee doesn...

  4. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...an organization such as COMPASS. Although COMPASS has a special interest in ocean science, they have good...Program at the University of California, Santa Cruz, has been turning scientists into confident and connected...

  5. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...Mentee. A Mentor will be more effective if the Mentee has done the self-reflection necessary to identify areas... you have nothing to give, think again. Everyone has knowledge to impart. An undergraduate can mentor ...

  6. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...trend in funding and space in academic research labs has more students looking for other options. While graduate... skills for alternative careers. Science Careers has developed a web-based, career-planning platform called...

  7. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ...learn about antibodies? Our new antibodies animation has all the information! Link in bio. #STEM #antibodies...

  8. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...measured using visible light scatter The optical system has both visible and fluorescent light sources. Visible...autofluorescence from your target cell type or that has arisen from fixation. Also include an isotype control...secondary antibody control in which the primary antibody has been omitted from the staining protocol. This control...gating during data analysis.  We hope that this blog has provided you with an overview of flow cytometry for...

  9. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...increase the sensitivity of detection (14-20). LDR has also been extended to multiplexed probe sets that...high-sensitivity multiplexed format (21, 27). This methodology has been used to rapidly screen buffer conditions for... 200 mM KCl at pH 8.5. This optimization method has been used internally at NEB to screen additional ...additional ligases, conditions, and formulations and has led to the development of the new HiFi Taq DNA Ligase....

  10. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...CRISPR/Cas editing toolbox for C. elegans Recent work has further improved the efficiency of genome editing... (Farboud et al., 2019). Work from the Mello lab has dramatically improved RNP editing with linear repair...elegans (Ebbing et al., 2017). Because C. elegans has an AT-rich genome, it can be difficult to find an...whereas import of CRISPR technology into C. elegans has been a transformative innovation for our community...

  11. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ..., organelles or other cellular impediments. FRET has been used in many applications, including drug discovery...up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it...synthetases is required and thus far, this method has proven most effective only in a few cell types (E...FRET measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you...

  12. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...the opsin you use); the efficacy of optogenetics has already been proven. Therefore, a concrete, well ...scientists who pioneered the development of optogenetics, has genetically manipulated a number of the opsin genes...kinetics of opsin activity/inactivity. Dr. Deisseroth has also generously made many of his optogenetic constructs...stereotaxic surgery (see videos below). The Deisseroth lab has developed many of these AAV constructs with various...

  13. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ... and allows targeting of specific cell types, it has a limited capacity to carry large DNA cargos. As ..., the search for smaller, compact genome editors has become a key focus in recent years. TnpB nucleases...nuclease domains (HNH and RuvC-like), and Cas12a has one (RuvC-like), TnpB also operates with a single...

  14. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ... newest kid on the block in the sequencing world has been long-read sequencing. Whereas the individual...auto-alignment, plus additional automation in the backend, has enabled the QC to keep up with the pace of deposits...plasmid. By working with the Data team, the QC team has also improved efficiency in many of their methods...

  15. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...stringently controlled and has a low copy number. Like the E. coli chromosome, it has a single ori and requires...Once the average copy number for a given plasmid has been reached, negative feedback circuits reduce replication...

  16. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...RNA and can express fluorescent proteins. This lab has created an automated tank for feeding and maintenance...scolopes (Joshua Rosenthal) The Hawaiian bobtail squid has an extreme number of nerve cells and an unusually... injection (hard to do, expensive, not every lab has the capability) with receptor mediated endocytosis...

  17. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... improve its stability in vivo. However, Spinach has proven useful in the engineering of riboswitches....syndrome (Strack et al., 2013). Since then, Spinach2 has been used to investigate intracellular viral DNA ...better choice for imaging than the monomer. Broccoli has been developed into a split aptamer that can be used...

  18. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

  19. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ... have been spent within a lovable community that has allowed me to expand my knowledge more than ever ... had opened its arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want...

  20. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...cells, but since the majority of the viral genome has been swapped for alternative genetic cargo, they ... there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available for...
Showing: 341 - 360 of 738 results