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We narrowed to 676 results for: Mos;

Showing: 341 - 360 of 676 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...pathways with sophisticated gene expression controls. Most cell types do not respond to low-intensity light...number of plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter ...

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...fluorescent proteins in common use (particularly not the most recently published ones), they can be a good starting...proteins the green and red proteins are generally the most detectable and so should be used to tag your least...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...goals. These first hires will set the tone and atmosphere that future hires will look up to." She also ... "Running Your Lab" booklet. What has been the most surprising thing about becoming a PI? Tom Ellis ...

  5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...integrated into the host genome upon viral transduction. Most transfer plasmids available from Addgene were derived...can be. Plasmids that encode SIN γ-retrovirus lack most of the U3 region of the 3’ LTR and are primarily...

  6. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...nuclear envelope, which occurs during mitosis. The most well-known retrovirus is HIV. Lentiviruses are ... Gene Therapy with AAV Figure out How to Get the Most from Your Lentiviral Transduction Additional Resources...

  7. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...the light microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging...Since I started my PhD, I have been convinced that most microscopy techniques can be replicated with commercially...

  8. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...available to the research community.    Validation For most cloning projects, the next step would be to validate...digest of the PCR products prior to cloning, which in most cases left the functional light chain intact, the...

  10. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...educational resources. Filters We identified the most useful kinds of information per resource category... details that make our new search engine unique. Most importantly, with this new implementation, we laid...

  11. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Overall, these approaches have two main drawbacks: 1) Most rely on transfection of more than one vector to ...lentiviral transduction, and AAV transduction.  In most of Zetsche et al’s experiments, a single vector ...

  13. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...promoters Chemically regulated promoters are among the most common inducible promoters. The positive inducible... agents to allow promoter activation. One of the most commonly used prokaryotic promoters is the negative...

  15. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    .... While some developers had science backgrounds, most did not. It was my job to translate complex lab ...front-end and back-end coding frameworks to use. I mostly sit and listen to stay informed on the details,...

  16. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...intrigued by the tRNA processing machinery present in almost every living organism. Precursor, or pre-tRNAs ...levels of gRNA expression. Since tRNAs are among the most abundant molecules in a cell, this system is clearly...

  18. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... a homologous template provided by the sister chromosome. As cells take those two ends and put them back... a technology that was difficult for all but the most experienced users and puts it in the hands of pretty...

  19. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...conjugate class than fluorophores are enzymes. The most common enzyme conjugates are Horseradish Peroxidase...be achieved through several different mechanisms. Most take advantage of chemical groups intrinsically ...

  20. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...isolate proteins, this blog will focus on one of the most common, purification based on the polyhistidine ...containing two or more adjacent histidines are present in most production systems and can bind and co-elute with...
Showing: 341 - 360 of 676 results