We narrowed to 621 results for: SCR

...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...Genome-Wide Control for Large-Scale Functional Screens        ...

... for over 10 years. Many successful scientists describe having a “posse” of mentors as one key to their...Ellen Daniell. In “Every Other Thursday,” Daniell describes a group of 7 women scientists who met every other...

...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle...

.... Such a system has been previously described for transcriptional activation/repression and genome editing...

...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

...video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...

...figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later,... Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource...

... fragments, these problems can be overcome by screening a higher number of potential clones. Golden Gate...In early 2011, the Bogdanove and Voytas groups described a new Golden Gate-based technology for genome ...

...Cre-dependent gene expression – placing a series of transcriptional and translational termination features with... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

...Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation of the...FRET probe, try searching PubMed for articles describing the FRET tool that you are looking for and check...

...cloning, the Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly...editing assemblies are sensor-actuators, either transcriptional regulators or chromatin-modifying enzymes. ...

...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

...efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017), they modified...undesired off-target editing effects. ABEs have been described to introduce base changes in cellular DNA and ...

...of citations per year and the number of other manuscripts that heavily cite the paper are displayed. You...the context of these citations. The number of manuscripts that cite the background, methods, or results...

...from the associated paper:   Color Base FP Description Article (Link to Plasmids) PI Blue EBFP2...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation...

...authors used a multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023... Figure 3: Overview of library generation and screening that led to identification and characterization...

...forward as quickly as possible and submitted our manuscript to the journal Cell just six weeks later. The...Asian-, African- and American-lineages). Strain discrimination is especially important in the current outbreak...

...there are any forms that need to be signed, or manuscripts that need to be reviewed, bring them to the meeting...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...

...sense, models can be both useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students...reaction to this model on his blog. Or, for a description of how effective this model was in explaining...