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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: SCR

Showing: 341 - 360 of 610 results

  1. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation of the...FRET probe, try searching PubMed for articles describing the FRET tool that you are looking for and check...

  2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...from the associated paper:   Color Base FP Description Article PI Blue EBFP2 EBFP2(1-10) and Capri...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation...

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...cloning, the Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly...editing assemblies are sensor-actuators, either transcriptional regulators or chromatin-modifying enzymes. ...

  4. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

  6. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...of citations per year and the number of other manuscripts that heavily cite the paper are displayed. You...the context of these citations. The number of manuscripts that cite the background, methods, or results...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...authors used a multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023... Figure 3: Overview of library generation and screening that led to identification and characterization...

  8. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...forward as quickly as possible and submitted our manuscript to the journal Cell just six weeks later. The...Asian-, African- and American-lineages). Strain discrimination is especially important in the current outbreak...

  9. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...there are any forms that need to be signed, or manuscripts that need to be reviewed, bring them to the meeting...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited...for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings...

  11. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...sense, models can be both useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students...reaction to this model on his blog. Or, for a description of how effective this model was in explaining...

  12. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...used to quantitate RNA levels using reverse transcription-qPCR (RT-qPCR). The first step of this process...requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified by qPCR...

  13. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...processes. To create the antibody toolbox, they screened antibody candidates using a synthetic human scFV...cross-reactivity, antibody candidates underwent affinity screening to select for slow off-rate binders. The best ...

  14. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...while seated or with your poster on your computer screen than it is to pretend you’re standing at the session...poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session...

  15. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...component as well as a small molecule which we will describe in more detail below. Types of Voltage Indicators...membrane, a concern with several of the previously described systems. Several adaptations of this method have...

  16. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...After this initial application, Stelzer’s group described the single-plane or selective-plane illumination...optical sectioning of a specimen is based on discriminating the out-of-focus reflected light by using a...

  17. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...well in plants and give similar stability to transcripts as the ones that people use all the time. Now... Marillonnet. ACS Synth. Biol., Just Accepted Manuscript, DOI: 10.1021/sb4001504. Journal. A modular cloning...

  18. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...expression) and a polyA signal (to terminate transcription) and the four constructs were then combined ...their 6-mer sequences, are rarely found in the transcriptomes of these species and can often be used to clone...

  19. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse...review. If you just want to use the nanobodies described in Pleiner et al, the key steps for generating...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that...Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires ...
Showing: 341 - 360 of 610 results