We narrowed to 617 results for: SCR

...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

.... Such a system has been previously described for transcriptional activation/repression and genome editing...

...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

...figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later,... Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource...

... fragments, these problems can be overcome by screening a higher number of potential clones. Golden Gate...In early 2011, the Bogdanove and Voytas groups described a new Golden Gate-based technology for genome ...

...Cre-dependent gene expression – placing a series of transcriptional and translational termination features with... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

...efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017), they modified...undesired off-target editing effects. ABEs have been described to introduce base changes in cellular DNA and ...

...authors used a multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023... Figure 3: Overview of library generation and screening that led to identification and characterization...

...from the associated paper:   Color Base FP Description Article (Link to Plasmids) PI Blue EBFP2...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation...

...cloning, the Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly...editing assemblies are sensor-actuators, either transcriptional regulators or chromatin-modifying enzymes. ...

...Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation of the...FRET probe, try searching PubMed for articles describing the FRET tool that you are looking for and check...

...of citations per year and the number of other manuscripts that heavily cite the paper are displayed. You...the context of these citations. The number of manuscripts that cite the background, methods, or results...

...forward as quickly as possible and submitted our manuscript to the journal Cell just six weeks later. The...Asian-, African- and American-lineages). Strain discrimination is especially important in the current outbreak...

...there are any forms that need to be signed, or manuscripts that need to be reviewed, bring them to the meeting...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...

...NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited...for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings...

...while seated or with your poster on your computer screen than it is to pretend you’re standing at the session...poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session...

...component as well as a small molecule which we will describe in more detail below. Types of Voltage Indicators...membrane, a concern with several of the previously described systems. Several adaptations of this method have...

...used to quantitate RNA levels using reverse transcription-qPCR (RT-qPCR). The first step of this process...requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified by qPCR...

...processes. To create the antibody toolbox, they screened antibody candidates using a synthetic human scFV...cross-reactivity, antibody candidates underwent affinity screening to select for slow off-rate binders. The best ...