We narrowed to 859 results for: STI

...service and is ready to use out of the box. We estimate that providing cloning grade DNA could save scientists...Plasmids offered as cloning grade DNA As we begin testing out this service, we’re starting with 210 plasmids...molecular biology experiments such as cloning, digestions, PCR etc. and/or directly long term storage. ...service in the future using the Cloning Grade DNA Suggestion Form.  Additional resources on the Addgene blog...

...science policy career and this seemingly simple question is a moving target. There are a multitude of options...policy? I’ve intentionally started with the ‘who’ question because the science policy community is the best...start. What is science policy? THE IMPOSSIBLE QUESTION. Science policy is the work you do to creatively... I would argue that this is the most important question that you can explore. The answer will help you...interviews or essays. Note: the answer to this question may change over time and that’s perfectly normal...

...hydrogen bonds and a base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined ... ́-phosphate to the ligation). The structural/mechanistic reason for this differential is not known for...Despite this higher fidelity, Taq DNA Ligase can still detectably ligate many T:G, T:T, and A:C mismatches...In this method, substrate pools were prepared consisting of one target (template) strand and four upstream...Reaction Temperature Calculator can be used to estimate an appropriate incubation temperature when using...

...development of phage-based therapeutics. After a brief stint at the venture capital firm, Flagship Ventures, ...opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens in food products prior to Sample ... from developing therapeutics to developing a diagnostic? 36:53 - 38:00 : As an entrepreneur, is it more...Read Sample 6's Paper on Bacteriophage-based Diagnostics Additional Resources on the Addgene Blog Check...

...plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries. We continue to ship...plasmids, analyze QC sequence, answer scientists' questions, manage our website, and continue to improve the...plasmids. The partnerships and collaborations with institutions and tech transfer offices that facilitate MTAs...improve, so thank you. 500,000x Thank You! Have a question? Email: [email protected] Call: +1 (617) 225...

...to be used as research tools is particularly interesting. Although humans have known about bioluminescent...bioluminescent organisms and their characteristic radiance for some time – there are records describing them dating...ring that glows with a faint green light. To investigate this green glimmer, Shimomura collected many,... Roger Tsien resulted in much of our current mechanistic understanding of GFP’s function. His lab described...

...microscope designs may further exploit the unique characteristics of fluorophores to block out of focus fluorescence...for a new sample I always start by asking two questions: 1) Is the sample dynamic or static? 2) Is the...the sample thin (< 15 μm) or thick? These questions will place each sample into one of four categories ...blurry out-of-focus light. The most difficult characteristic to capture in these types of samples is their...from a well-established microscopist at your institution when heading into a new set of experiments. ...

...proteins, so you can't distinguish the different proteins from each other. To distinguish different target ...inside the cells and are thus typically used for distinguishing live from dead cells. The combination of antibody-fluorophores...fluorophore? Strong brightness makes it easier to distinguish a signal from noise; therefore, it is recommended...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

...Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways...representing core nodes that, when over-expressed, constitutively activated or inactivated the pathway. “The ...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...pathways. With support from the National Cancer Institute, they expect to add another 200 or 250 constructs...

...Maintaining Institutional Knolwedge and Continuity - SITN's key technique for retaining institutional knowledge...mosquitoes, allergies, & more! We briefly answer the question, will the effects of climate change make put Cambridge...junior and one senior.  To learn more about the STIN's podcast, email [email protected]    For more...

... Twitter.” This paper addresses the following questions about scientists on Twitter: who are they? What...they are based on the US Occupational Employment Statistics (OES), but international Twitter users were included...orange)  scientists largely cluster by discipline, suggesting that scientists tend to follow scientists within...out the Top 50 Science Stars on Twitter Want suggestions on how to use social media as a scientist? Check...

...                           National Institute for Physiological Sciences Broad Institute                                       ...Hospital                           California Institute of Technology             Johns Hopkins University...Academy of Medical Sciences University of Texas at Austin University of California, San Francisco  University...

... the panelists were interesting enough to make up for the somewhat naïve questions. During the panel, ...their seminal, cooperative paper in Science.  Interestingly, this project started with their labs studying...for billions of years (…) Bacteria are really sophisticated”.     New to CRISPR/Cas9 Technology? Learn ...

...14] Hepatocytes [15] Pancreatic β-cells [16] Intestinal tissue [17] Lung epithelial cells [18] Red ...approaches differ in several ways. Below is a table listing some of critical differences (adapted from Zhou...Zhou and Melton, 2008, [43]): Characteristic Reprogramming+ Transdifferentiation in vivo or in vitro...differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 2011. 470(7332): p. ...37. Merrell, A.J. and B.Z. Stanger, Adult cell plasticity in vivo: de-differentiation and transdifferentiation...

...property by including ccdB on its destination plasmids. In the destination plasmid, the ccdB gene is flanked... interest by taking advantage of bacterial characteristics such as prototrophy, the ability to synthetize...sites. To introduce a gene of interest into the destination plasmid, recombination sites must first be added...

...composed of camels, llamas, and alpacas (and their undomesticated counterparts) - not exactly species that jump... is a lot of excitement around using sdAbs in diagnostic applications. For example, fluorescently and ...generate new sdAbs. There has also been some interesting work done developing transgenic mice that express...S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https...

... fastest way for us to release your plasmids is still for you to notify us as soon as your article is ...a project isn’t working out as planned, but you still hope somebody will find the plasmids useful, we’...article, a preprint, or without any paper at all. Posting preprints and openly sharing materials are both...think. We appreciate your feedback. If you have questions about depositing, check out our website, Help ...

...mitigating noise generated by off target digestion. After MNase digestion, fragments are released from nuclei... DNA around the target protein. This targeted digestion is controlled by the addition of calcium, which...measure of DNA binding sites in combination with existing ChIP-seq data sets, of which there are many readily...

... some special characteristics of scientists in the work place as described by Sebastiano Massaro in his...some of these common pitfalls of management and still not get the most from your team. It is pretty clear...learned to get results with the carrot, and not the stick.     This is the first in a 5 part series on Introduction...

...are on the right track if they start asking you questions. Remember that people like a good story and science... this case you – on a quest to ask and answer questions and hopefully discover something new. Science ...out to science writers in your area or at your institution; the news or communications office is a good ...internship at a place like Science News. Ask your institutional news office if there might be opportunities ...