We narrowed to 351 results for: SUA
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TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m.... will first need to transfect producing cells, usually 293T cells, with your transfer plasmid and your...
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Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m.... digestion products (which could be easier to visualize and identify). If you need/want to put the digestion... -
A Guide to Getting Started in Undergrad Research
TypeBlog PostPublishedJune 8, 2021, 1:15 p.m....and responsibilities can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....mismatches and cleave them. The results are then visualized using gel electrophoresis. Surveyor™ and T7E1... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....available from commercial vendors. These libraries usually contain 4-12 gRNAs/gene, which results in libraries... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....effectors, like Cas9. The Cascade complex doesn’t usually carry out the function of the specific CRISPR type... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....chromosomal integration modules, and many more of the usual suspects. The Multiplex Yeast Toolkit builds on ... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m....not be partisan: everyone (of every political persuasion) must be humble in the face of evidence. Stephanie... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....antigen interactions (Saraheimo et al., 2013), and visualizing structural changes in the ribosome during protein... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog PostPublishedSept. 10, 2020, 11:45 a.m....system, editing or labeling efficiency doesn’t usually change when you add multiple gRNAs on one plasmid... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....this “non-target strand.” To avoid confusion, we usually use different terminology when discussing base ...