We narrowed to 612 results for: Sis
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TypeBlog PostPublishedMay 7, 2020, 1:15 p.m.... starting point for a new generation of ABEs), consists of 3 components: a deaminase (TadA, originally...edited the fetal hemoglobin promoter and caused persistent expression of fetal hemoglobin to a greater level...
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A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....topics that may require additional attention during analysis. These include topics such as: locations in a ...on Addgene.org Educational Resources Sequence Analysis of a Plasmid Protocol ... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m.... will be selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic... may be some low-level fluorescent protein or resistance marker expression following transfection, higher-level... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog PostPublishedJuly 14, 2016, 2:30 p.m....rates, determined using sophisticated genome-wide analysis. At many of its computationally predicted off-... Hur JK, Been KW, Yoon SH, Kim JS. Genome-wide analysis reveals specificities of Cpf1 endonucleases in... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination of the transcript...: PMC102582. Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion... -
The Materials Science of Optogenetics Experiments
TypeBlog PostPublishedSept. 17, 2015, 2:30 p.m....will be erratic, opsin activation/inactivation inconsistent, and behavioral results impossible to interpret...sequentially increasing fineness helps to maintain a consistent stream of light. Test your implant by connecting... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog PostPublishedAug. 6, 2015, 2:30 p.m....transferable skills such as leadership skills can only assist you and make you more qualified for future career...blogger Juliet Moncaster! Juliet Moncaster is an Assistant Professor at the Boston University School of Medicine... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....the cell pellet, I lyse the cells with this AAV lysis buffer and freeze/thaw the lystate four times in...-on time and 2 hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m.... popular cloning methodologies, with a special emphasis on these five categories. Please note that many...backbone containing a topoisomerase I cut site, toposisomerase I, and a taq polymerase-generated insert. When... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....to participate in genetic recombination during meiosis. Also, flies are not viable if they are homozygous...that males do not recombine chromosomes during meiosis. This can sometimes be a handy feature when setting... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m.... increase efficiency of these systems, though consistent expression can still be a challenge. A multitasking...Dolan, A. E., Liao, M., & Ke, A. (2018). Structure basis for RNA-guided DNA degradation by Cascade and Cas3... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m.... easier, and more comprehensive thanks to the consistent development and improvement of CRISPR-based technologies...& Platt, R. J. (2023). Transcriptional linkage analysis with in vivo AAV-Perturb-seq. Nature, 622(7982... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....the virions out. We recommend using a Tris-based lysis buffer and sonication. The obtained virions from...can then remove the density gradient material by dialysis/buffer exchange and further concentrate the virions... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....you can adjust compensation values later in your analysis, unlike voltages. Figure 1A shows a single-color...always change your compensation values during the analysis portion of your experiment. So, as long as you... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....requesting each year (Figure 2). We did a similar analysis with CRISPR pooled libraries, although we used... from this data is that CRISPR technology is consistently showing rapid development and quick adoption... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated a rate-limiting... a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent protein ... Single Fluor Chemigenetic Sensors This system consists of a genetically expressed enzyme, typically on... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....PMID: 14999088. 7. Turetsky, T., et al., Laser-assisted derivation of human embryonic stem cell lines ...from IVF embryos after preimplantation genetic diagnosis. Hum Reprod, 2008. 23(1): p. 46-53. PubMed PMID... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....difficult” GC-rich regions of the genome, which seem resistant to Cas9 cleavage (1). Both the CRISPR-Cas9 and...however rely on cloning into a vector or in vitro synthesis, which could be less desirable if one has to target... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m.... a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to easily screen...thought to function by making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element...