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We narrowed to 696 results for: Tec

Showing: 341 - 360 of 696 results

  1. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated virus serotype...adeno-associated virus vectors with sensitivity sufficient to detect single infectious events. Human Gene Therapy, 15...addgene.org  AAV Titration by qPCR Using SYBR Green TechnologyAAV Titration by ddPCRAAV Purification by Iodixanol...

  2. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ... where rhodopsin functions as both the voltage detector and fluorescent signaler. The pro of this system...several targeting domains. The quencher and voltage detector in this system was dipicrylamine, a lipophilic...high-speed, bright, red fluorescent voltage sensor to detect neural activity. Sci Rep 9, 15878 (2019). doi: ...

  3. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology...discovery through the creation of innovative technologies. To date his group has develop several popular...catalytically active Cas9 nuclease." Nature biotechnology (2015). PubMed PMID: 26436575. PubMed Central...

  4. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ... on and so forth. This also serves as a light technical control - an RQ value of 1.5 means you have either...chemistry (see image, left, of droplet immersion technique, courtesy of CAR15.) Each droplet contains a primer...Molecular Biology Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll SA. ...

  5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...plasmid, using the fragment exchange (FX) cloning technique which uses Type IIS restriction sites (Geertsma...protein of interest. The Seeger lab used the ELISA technique described in their protocol because it is less...protein is added and after a few washes, binding is detected using a colorimetric reaction. After the ELISA...

  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...understanding of the fast, deadline-driven pace of biotech. In other words, industry postdocs already have...Biogen, Amgen, New England Biolabs, Regeneron, and Genentech, among others, and the list is growing. Some post... a position open up. Other companies, such as Genentech, will not. The point is that each company has ...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...the AAV production process. Either SYBR green technology or a TaqMan primer/probe set can be used and ...sample for the DNAseI digest. Use good sterile technique when handling your AAV plasmid standard and AAV...Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated virus serotype...

  8. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...Advantages of the agarose cloning method The agarose technique should be useful to scientists who wish to rapidly... from a population of transfected clones. The technique is applicable to a wide variety of cell lines,...physiological properties. An advantage of this technique is the potentially large number of single clones...

  9. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing purposes...cheaper, faster, and easier than prior editing techniques. More and more CRISPR tools are being published...how you will evaluate the outcome: Will you be detecting insertions/deletions using a mismatch repair assay...

  10. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...therapy. The year was 2001 and I started a job as a technician in a lab using adeno-associated viral vectors... I quickly became fascinated by this emerging technology and its potential for the treatment of some genetic...research is being done to further improve the technology: engineering of new capsid variants, designing...

  11. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...club occurs with a small technical team (single lab or team), a deep technical dive on single papers could...must stay current with literature and emerging technologies to remain competitive and relevant. Enter: the...

  12. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...experimental process. The majority of current cloning techniques involve the assembly of a circular plasmid in... 1991, Bubeck et al., 1993). But why did this technique fail to gain traction? It is likely that both ...ends on DNA using polymerase chain reaction." Biotechniques 10.1 (1991): 62-66. PubMed PMID: 2003926. 2....

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...CNS and PNS.That is until the Gradinaru Lab at Caltech stepped up to the challenge. Find the PHP Plasmids...Recombinase-based AAV Targeted Evolution. In this technique, a pool of mutant AAV capsid (cap) expressing ...widespread gene transfer to the adult brain. Nature biotechnology. 2016;34(2):204-209. PubMed PMID: 26829320. ...

  14. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...key steps to colony PCR are: 1) design primers to detect the presence of your insert; 2) set up a standard...Running a no template control PCR reaction for detecting contamination is also a good idea.    Analyzing....org Read about Choosing a Molecular Cloning Technique Learn to Perform a Bacterial Transformation Deposit...

  15. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...reduce this likelihood. The key problem with these techniques is efficient delivery, as the antimicrobial must...efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed: PMID 252409288...produce sequence-specific antimicrobials.” Nature Biotechnol. 32(11): 1146-50. (2014). PubMed: PMID 25282355...

  16. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...offer the maximal biosafety available for the technology. The minor limitation in using the 3rd-generation...scientists often refer to as “retroviruses” are technically gamma-retroviruses - another, separate member...transfer vectors for transient transfections? A2: Technically, yes. Will it actually work? That depends. The...

  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...Leehyo Bioscience 2015 Korea San Diego Biotech Lab 2018 Mexico e-nnovation Life Sciences...Sciences 2020 India Sinapse Biotecnologia 2022 Brazil   In some cases, the distributors broadly...best part of any conference is scientists, from techs to grad students to professors, stopping by to say...

  18. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...two deposits that increase the utility of this technique.  By engineering a light-sensitive LOV domain ... tapes for intracellular recordings. Nature Biotechnology, 41(5), 631–639. doi: 10.1038/s41587-022-01524... using thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently...

  19. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...integrate the mARGs using PiggyBac. Gas vesicles are detectable under ultrasound and phase contrast microscopy...bioengineering. They are both members of the Shapiro lab at Caltech.   References and Resources References Piraner...monitoring of tumors and tumor-homing bacteria. Nat Biotechnol 1–13 (2023) doi:10.1038/s41587-022-01581-y   ...

  20. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...the Dow lab created a base editing reporter that detects and enriches for base editing events in vivo without...conversion) they could induce the translation of any detectable protein, not just fluorescent proteins. ...protein expression, it can be used for an array of detectable proteins including fluorescent proteins, antibiotic...
Showing: 341 - 360 of 696 results