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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 629 results for: UTY

Showing: 341 - 360 of 629 results

  1. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...Flame Award on Bluesky. In 2016, we also began distributing ready-to-use viral vectors from some of our ...

  2. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...improve localization accuracy when used in super-resolution microscopy by reducing the distance between the...fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https...

  3. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...that of the protein of interest. A high imidazole elution buffer disrupts the interaction of the protein ... disrupt contaminant-metal ion binding without eluting the target protein and needs to be empirically ...

  4. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...responses are also a concern when it comes to RNAi therapeutic delivery. Thus, balancing the amount of siRNA...just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease, and much more...

  5. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding proteins that...proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-...

  6. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ..., broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules...

  7. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making it easy ...PMCID: PMC4253859. 4. Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific...

  9. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ... N., et al. "Tumor cells can follow distinct evolutionary paths to become resistant to epidermal growth...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene...

  12. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...Agrobacterium Ti virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative...Resistance Genes Antibiotic Resistance: An Old Solution but a New Problem Plasmids 101: What is a Plasmid...

  13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...hosted by Nature), and Addgene are open to guest contributions. If you decide to start your own blog, check...provides on-site courses in public engagement for institutions and the Royal Society gives training in media...

  14. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...or licenses that could prohibit Addgene from distributing the plasmid, select “yes” from the drop-down...but can accept sequence files in any format. Distribution status for your plasmids: Hold for Publication...

  15. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...that you will need large volumes of lentivirus routinely, you may opt for simply increasing the number ...ultracentrifuge, PEG will precipitate all proteins in a solution, not just the lentivirus; consequently, this may...

  18. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...versatile tool for genome engineering, but its utility is dependent on its ability to infiltrate cells... like the addition of a fluorophore or tag. To utilize HDR, a donor template containing the desired sequence...

  19. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...Conditional gene-targeting in mice: problems and solutions." Immunity 48.5 (2018): 835-836. PubMed PMID: ...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...mammalian cells. It's based on the introduction & resolution of two DSBs in a fluorescent reporter. Find ...
Showing: 341 - 360 of 629 results