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Showing: 341 - 360 of 621 results

  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making it easy ...PMCID: PMC4253859. 4. Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific...

  3. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ... N., et al. "Tumor cells can follow distinct evolutionary paths to become resistant to epidermal growth...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene...

  6. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...or licenses that could prohibit Addgene from distributing the plasmid, select “yes” from the drop-down...but can accept sequence files in any format. Distribution status for your plasmids: Hold for Publication...

  7. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...that you will need large volumes of lentivirus routinely, you may opt for simply increasing the number ...ultracentrifuge, PEG will precipitate all proteins in a solution, not just the lentivirus; consequently, this may...

  10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...versatile tool for genome engineering, but its utility is dependent on its ability to infiltrate cells... like the addition of a fluorophore or tag. To utilize HDR, a donor template containing the desired sequence...

  11. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...Conditional gene-targeting in mice: problems and solutions." Immunity 48.5 (2018): 835-836. PubMed PMID: ...

  12. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...Agrobacterium Ti virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative...Resistance Genes Antibiotic Resistance: An Old Solution but a New Problem Plasmids 101: What is a Plasmid...

  13. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...through qualitative data that they are a long-term solution to reproducibility and cost-effectiveness for ... innovation by allowing scientists to find and utilize reliable, high-quality antibodies. We are creating...

  15. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...mammalian cells. It's based on the introduction & resolution of two DSBs in a fluorescent reporter. Find ...

  16. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...hosted by Nature), and Addgene are open to guest contributions. If you decide to start your own blog, check...provides on-site courses in public engagement for institutions and the Royal Society gives training in media...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...the intramolecular dynamics of the proteins in solution and when associated with microtubules. ...sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  Figure...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...get you down for too long, though, because many solutions have been found to get around this restriction...Saur, D., & Rad, R. (2022). Tutorial: design and execution of CRISPR in vivo screens. Nature Protocols, 17...

  19. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... virus of interest causes cancer, as well as therapeutic strategies to target it, than you typically would...outline: Instead of getting bogged down in the minutia or coming off as a broken record, remember the ...
Showing: 341 - 360 of 621 results