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We narrowed to 643 results for: Vars

Showing: 341 - 360 of 643 results

  1. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your samples... research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene...

  2. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ..., we are here to help with resources from AAV variables that matter to adenovirus production tips. For...DOI:10.3390/v13030388 Coffin J.M., Hughes S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold...

  4. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...protein modifications of interest or any of a huge variety of ncAAs to study the relationship between structure... our blog – we’ll be posting more posts on the various protocols and helping to spread the word as new...

  5. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...Excitation (dotted) and emission (solid) spectra for various teal, green, yellow, orange, and red fluorophores...Figure 4: Calcium titration curves for OCaMP and various red and yellow GECIs measured with soluble protein...

  6. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...

  7. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... versus other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...

  9. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...exhibition area, I got a sampling of the broad variety of tools in neuroscience trade. There were, of ...easily I could jump in and ask questions about the various posters I visited. If you’re interested in neuroscience...

  13. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...developed a modular suite of plasmids (pGT) with various replicative origins that can be used for broad ... host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible...

  14. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti Monster, and the Chicken IgY domain...

  15. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...   Ask the learners themselves for feedback in a variety of ways, like having them restate or summarize ...you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

  16. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance...

  17. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...to apply the scientific method to problems in a variety of contexts.  How do you develop problem solving...

  18. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    Published
    Oct. 21, 2025, 1:15 p.m.
    ...reactions that work in just about any situation (Devaraj & Finn, 2021). In one of the most common Click ...clicking. References and Resources References Devaraj, N. K., & Finn, M. G. (2021). Introduction: Click...

  19. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

  20. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...
Showing: 341 - 360 of 643 results