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We narrowed to 642 results for: Vars;

Showing: 341 - 360 of 642 results

  1. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...after the conjugation reaction is done, through a variety of methods or commercial kits.  However, antibodies...create a small, usable panel.  First, fluorophores vary in brightness. You don’t want a super-bright fluorophore...

  2. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ..., we are here to help with resources from AAV variables that matter to adenovirus production tips. For...DOI:10.3390/v13030388 Coffin J.M., Hughes S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold...

  4. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...protein modifications of interest or any of a huge variety of ncAAs to study the relationship between structure... our blog – we’ll be posting more posts on the various protocols and helping to spread the word as new...

  5. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...

  6. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...Excitation (dotted) and emission (solid) spectra for various teal, green, yellow, orange, and red fluorophores...Figure 4: Calcium titration curves for OCaMP and various red and yellow GECIs measured with soluble protein...

  7. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... versus other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...

  9. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused on current ...100 teachers each year, with workshops held at various locations around the U.S. (and Mexico in 2018)....

  12. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

  13. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti Monster, and the Chicken IgY domain...

  14. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...developed a modular suite of plasmids (pGT) with various replicative origins that can be used for broad ... host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible...

  15. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...   Ask the learners themselves for feedback in a variety of ways, like having them restate or summarize ...you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

  16. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance...

  17. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...to apply the scientific method to problems in a variety of contexts.  How do you develop problem solving...

  18. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

  20. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...interest. Thanks to the tremendous efforts made by various research teams to build and constantly improve ...integrated, we look forward to the realization of a variety of perturbations and applications in functional...
Showing: 341 - 360 of 642 results