We narrowed to 644 results for: Vars;

...plasmids can direct the production of virus with various tropisms.  Packaging using helper-free packaging... virus is produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables ...

...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your samples... research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene...

... confirm expression in target cells. While the various AAV serotypes can be used to achieve different ...supply of transgenic reporter lines that supply a variety of GFP expression patterns, thus allowing you to...

...Different setting, different population, new genetic variation, or different handling can all be important.  ...certain that some important, previously unknown variable actually makes a big difference for the robustness...

...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

..., we are here to help with resources from AAV variables that matter to adenovirus production tips. For...DOI:10.3390/v13030388 Coffin J.M., Hughes S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold...

...protein modifications of interest or any of a huge variety of ncAAs to study the relationship between structure... our blog – we’ll be posting more posts on the various protocols and helping to spread the word as new...

...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...

...after the conjugation reaction is done, through a variety of methods or commercial kits.  However, antibodies...create a small, usable panel.  First, fluorophores vary in brightness. You don’t want a super-bright fluorophore...

... versus other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...

...Excitation (dotted) and emission (solid) spectra for various teal, green, yellow, orange, and red fluorophores...Figure 4: Calcium titration curves for OCaMP and various red and yellow GECIs measured with soluble protein...

... available.  We’ve also had some requests for variations on the plasmids we’ve developed. We’re working...be doing something similar. There may be other variants of this that are better for some experiments than...

...the degree to which genes are turned on and off varies depending on which guide RNA we use. What we have...straightforward to apply the new library for use in various cell types and in live mice. Therapeutic uses in...

...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti Monster, and the Chicken IgY domain...

...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

...   Ask the learners themselves for feedback in a variety of ways, like having them restate or summarize ...you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance...

...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...to apply the scientific method to problems in a variety of contexts.  How do you develop problem solving...

...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

...developed a modular suite of plasmids (pGT) with various replicative origins that can be used for broad ... host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible...