...Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains Reagents developed during the SARS-...the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids... availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley...mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution, has been able...clinical manifestations, transmissibility, and laboratory studies of COVID-19: an observational study. ...

...PMCID: PMC49545. 3. Coffin J, Hughes S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold ...applications to help you start (safely!) thinking about the possibilities. Ready to plan your next (or maybe...Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

... The DNA packed into our chromosomes is very much 3-dimensional, as you can see by this post’s chromatin...Leah Bury of Microscopic Art. I started thinking about this problem when I recently attended the 2018 Chromosome...lot of scientists who not only remember to think about DNA in this context, but also specifically study...plasmids containing these genes. As with the PIs above, many of these genes were discussed at the 2018 ...chromatin This is not the first Addgene blog post about chromatin--multiple Addgene colleagues and guest...

... dementia-associated tau, delivering dCasRx and a 3-spacer array targeting the MAPT exon 10 splice acceptor...species (read Arbor Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx...this paper are available at Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources... Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using Cas9 Transcriptional...Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources on Addgene.org: Find...

...wrong, replace them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether...cloning before you can start your experiment? What about all the reagents you need to order? Sometimes, it...dive deep into generating the data you’re excited about. When we were closing in on the one million plasmids...million plasmids shared than not!), we think it’s about time we updated this post. How much time does ...choose the right cloning method Find blog posts about scientific sharing here Download the Plasmids 101...

...the insert is large, we may only check the 5’ and 3’ ends to verify that the sequence assembly and identity...One of the best things about sharing plasmids through Addgene is that we provide an added level of confidence...bp of clean sequence from a typical reaction, or about 250 amino acids worth, so make sure the feature ...96-well microplates (identical copies, see image above). We then select forward and reverse primers for...specifically to target this region, as outlined above. Avoid primers that bind within or must read through...

....  For example, the phosphorylation status of SRC-3 defines its association with other members of the ...a broad range of reproductive, developmental, metabolic, and immune response programs. NRs also encompass...gallstone disease, senescent illnesses, and the metabolic syndrome. Coregulators Coregulators constitute... regulatory regime (such as genes involved in metabolism, growth or cytokine action). Primary or core ...and the free exchange of these vectors between laboratories. The development of the transient transfection...

...biology, technology and application. Viruses. 2011 Jun;3(6):677-713. DOI: 10.3390/v3060677 Varanda C, Félix...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,... be helpful to you. Interested in learning more about a specific virus? We have you covered with guides..., adenovirus, and AAV. If you’re wondering more about what applications these viruses are good for, we...

... to wane about a month later, the animal is given additional booster immunizations every 2-3 weeks to ...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...

... PMID: 14166766. PubMed Central PMCID: PMC300121. 3. Söderberg, Ola, et al. "Direct observation of individual... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

...and best characterized of these is the Src homology 3 (SH3) domain family, known to be involved in cytoskeletal... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...can take as long as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used...

... to antibody response 2) very high immunogenicity 3) and long-lasting immune response even after a single...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

...PMID: 21364278. PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene ...Resources on the Addgene Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service...

...synthetic biology tools for cyanobacteria. Figure 3: Assembly of plasmids using the Cyanogate Kit and ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

...10) and grow them in a small culture volume (2 or 3 ml) containing the corresponding antibiotic, extract...they get old, they lose competency). If all the above is correct, you should probably have a second and...blog posts Read blog posts on plasmid cloning Read about different plasmid elements Resources on Addgene.org...

...ssRNA engaging one strand of a dsDNA molecule; the 3-stranded structure produced is known as an R-loop....power of dsRNA for a long time, even before we knew about all the diverse in vitro production mechanisms. ...repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular processes...

...PubMed PMID: 2839833. PubMed Central PMCID: PMC281709. 3. Tissue– and site–specific recombination in transgenic...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

...spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other species can recognize...our CRISPR 101 blog posts Find more information about Cas proteins Download the CRISPR 101 eBook Resources...

...transfection. The other (mKate2) is regulated in the 3’ UTR by four miRNA target sites and thereby serves... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...

...sequences more efficient and less risky.   Fig. 3: Development of a cleavage-free dCas9-based gene editor... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. ...