We narrowed to 911 results for: addgene

...after editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair...between 1 to 2 kb in length. There are plenty of Addgene plasmid options for templates - see the example...construct as described in Figure 2.  You can also use Addgene plasmids to purify Cas9 protein. The table below...How to design Easi-CRISPR repair templates using Addgene plasmids. Design PCR primers to add T7 promoter-left... PMCID: PMC5828090 Additional Resources on the Addgene Blog Read Our Previous Post on CRISPR Mouse Models...Ribonucleoprotein (RNP) Delivery Resources on Addgene.org Check out our CRISPR Guide Find Plasmids for...

...of the Tet-Off system.  For more information on Addgene Tet plasmids, see our Tetracycline resource page...PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez, Alberto, Dominic Didiano...PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, Christopher J. Potter, Liqin Luo &...PMC3846601. Find plasmids from this publication at Addgene. Riabinina, et al. “Improved and expanded Q-system...PMC4344399. Find plasmids from this publication at Addgene. Riabinina O, Potter CJ. The Q-System: A Versatile...PubMed PMID: 22129153. Additional Resources on the Addgene Blog Quick guide to working with Drosophila Terminators...and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline Collection ...

...plants available through Addgene. In addition to the plasmids described above, Addgene carries several useful... are available to academic researchers through Addgene. CRISPR components for plant genome engineering...expression of small RNAs in their respective species. Addgene carries >30 “empty gRNA” backbones which contain... or ubiquitin promoters (Belhaj et al., 2013). Addgene carries Cas9-containing plasmids for knockout, ...s41580-020-00288-9 Additional Resources on the Addgene Blog Read Up on CRISPR Multiplexing Tools Validate...Edit Design Your gRNA Additional Resources on Addgene.org Find CRISPR Plasmids for Plants Read Our CRISPR...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...protein. The pCri system, recently deposited to Addgene by F. Xavier Gomis-Rüth, aims to take the guesswork... either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The ...visualization of the data from RSCB PDB Resources on the Addgene Blog Plasmids 101: E. coli strains for protein...expression Plasmids 101: Protein tags Resources on Addgene.org Find Additional Cloning Kits ...

...demonstrate the new system in a video filmed at Addgene:     If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References...

...019-46171-2. Resources on Addgene.org Modular Cloning (MoClo) Guide Addgene’s eBook Collection More resources... will review common uses of MoClo and the kits Addgene offers to kickstart these applications, no matter...starter for your general plant cloning experiments. Addgene also has two additional MoClo plant kits: Plant...CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too. The AspFlex... (Brychcy et al., 2023). For plant biologists, Addgene has not one, not two, but three CRISPR MoClo kits...study in yeast cells. Need more MoClo? Check out Addgene's MoClo Guide! Customizing MoClo for your needs ... resources on the Addgene blog Plasmids 101: Golden Gate Cloning High Complexity Golden Gate Cloning ...

...If you’re been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you...placing the aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the... we’ll take a deeper dive into the content of Addgene’s aptamer soup by exploring the development and ...and applications of fluorescent RNA aptamers in Addgene’s collection. Green fluorescent aptamers Spinach...PMCID: PMC4143336. Additional resources on the Addgene blog Learn more about aptamer fluorophores Investigate...Find other uses for RNA aptamers Resources at Addgene.org Browse fluorescent protein plasmids and resources...

... an ambitious goal of curing Rett Syndrome and Addgene is thrilled to partner with them to launch a Rett...passion for facilitating research and discovery, Addgene and RSRT have consolidated and centralized a collection...Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith Joung, PhD, and...the root cause of Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource Center will...deposit their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to...

...sequencing, and optical pooled screens in human cells. Addgene offers vectors for CROPseq-multi using selection...recombinant antibodies were recently added to the Addgene repository of tools for SAR-CoV-2 research. Two...critical component for viral genome packaging. Addgene recommends both for use in western blots (Figure...as a loading control (right panel). Image from Addgene. These antibodies are recombinant versions ... out the new and noteworthy tools featured in Addgene’s Neurodegeneration Research Collection. This page...

...Are the shots clear enough for the audience? As Addgene’s Science Video Production Specialist, I’m constantly...March 2020, just four months after I started with Addgene, I was about to have a check-in with my boss Jennifer...traditional solution was completely out of reach for Addgene.  So, onto research! I needed to design a system...the Lab Design: The lab is the beating heart of Addgene. It’s why we exist, storing plasmids, and distributing... out all of the written and video protocols at Addgene? If you need help in the lab, we have you covered...some incredibly strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure...

...Mess in IHC Resources on Addgene.org  Browse ready-to-use antibodies at Addgene...visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? If so, you are not alone. Full-sized...when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken up, there...j.crmeth.2022.100301  Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment...

...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services...Services  Resources on the Addgene blog  Viral Vectors 101: Biosensors Viral Vectors 101: Viral Applications... to AAV Find AAV Calcium Biosensor Vectors at Addgene ...

...Resources Resources on Addgene.org Addgene viral services Addgene Biosafety Guide Addgene Viral Plasmids ... Type of Virus Should I Use? Resources on the Addgene blog Viral Vectors 101: Types of Viruses Viral...

...References on Addgene.org Check out Addgene's Chemogenetics Guide Find fluorescent proteins at Addgene ...fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent Proteins 101: GFP Fusion Proteins...0471140864.ps0909s73 Additional references on the Addgene blog Browse Fluorescent 101 blog posts Read about...

...More Resources at Addgene:  Check out more Plasmids 101 Posts! Check out Addgene’s restriction cloning...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...can also be adapted to express multiple gRNAs. Addgene has two Golden Gate-based gRNA assembly methods...

...manipulation Resources on Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors...tools for optogenetics and chemogenetics on the Addgene website. Optogenetics also requires that light ...bs.mie.2018.01.022 Additional resources on the Addgene blog Read more about optogenetics New to viral...

...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration...online and in-person, you helped us understand how Addgene can better serve the global scientific community...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...Children’s Hospital and Harvard Medical School. Addgene is proud to present a video reprint of the CRISPR...the full protocol from the JOVE article here on Addgene's website and find more CRISPR resources.    Thank...Browse CRISPR Plasmids CRISPR Information at Addgene More CRISPR-Cas Blog Posts    ...