We narrowed to 462 results for: bli

1. OCaMP: A New Calcium Indicator for Neural Imaging

   Type

   Blog Post

   Published

   Aug. 19, 2025, 1:15 p.m.

   ...lasers, with two-photon excitation at 1030 nm, enabling two-photon imaging without compromising on brightness...

2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   Published

   Sept. 26, 2024, 1:15 p.m.

   ...option is to perform a hierarchical assembly, assembling fragments in multiple steps to avoid using multiple...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition...

4. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA...

5. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ...

6. A Deep Dive into BioBuilder

   Type

   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   .... (and Mexico in 2018). BioBuilder textbook, published by O’Reilly Media. BioBuilder's open-access curriculum...

7. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ... gene. Mismatch nuclease assays are completely “blind” to this very important difference (Figure 3). ...

8. Addgene’s Viral Service - Why Virus? Why Now?

   Type

   Blog Post

   Published

   March 23, 2017, 2:30 p.m.

   ...Wyss Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral...

9. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...Stasevich and Hiroshi Kimura’s labs have recently published a set of anti-FLAG frankenbodies, a flexible, ...

10. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...containing lists of plasmids from the indicated publications. You can request all of these plasmids directly...

11. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment...

12. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...and joined each fragment with one part of an established pair of proteins (iLID and SspB) that undergo...

13. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ... from this collection in your experiments and published your data, please fill out this form and we’ll...

14. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...expression under low temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic...

15. Behind the scenes: Addgene’s new search engine and more

    Type

    Blog Post

    Published

    Dec. 12, 2019, 2:22 p.m.

    ... higher than matches of the same name in the publication field. Suggestions We provide as-you-type suggestions...

16. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type

    Blog Post

    Published

    Feb. 19, 2019, 1:36 p.m.

    ...accounting from Babson College. He is a certified public accountant (CPA), certified information systems...

17. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ...selected growth conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically...

18. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies...

19. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ... sequence (“microhomologies”). NHEJ doesn’t obligatorily introduce indels. Given the end structure of...

20. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ...Congratulate your connections when they get a new paper published. Stay in touch enough to plan to meet up at the...