We narrowed to 812 results for: cha;

... studying protein turnover is radioactive pulse-chase experiments. However, these experiments are problematic...synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of Sciences...PubMed Central PMCID: PMC4414919. 5. Yarmolinsky, Michael B., and L. Gabriel. "Inhibition by puromycin of...Single β-actin mRNA detection in neurons reveals a mechanism for regulating its translatability." Science 343.6169...

...and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based...coupling can be achieved through several different mechanisms. Most take advantage of chemical groups intrinsically...that make up the disulfide bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies...References Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK (2012) A deep profiler’s guide to cytometry...

...restriction enzymes or vector purchases (Table 1). Restriction enzymes and vector purchases can raise the cost anywhere...one-off projects, or even save yourself hours by chatting quickly with the postdoc who’s really good at ...harder it is to throw in last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means...

...CRISPR/Cas9 genome editing can result in unwanted changes at non-target sites. Means to decrease these so-called...between the non-target strand and a positively charged groove formed by the Cas9 HNH and RuvC nuclease...al reasoned that, if they decreased the positive charge in the HNH/RuvC groove, then they could weaken ...interactions between the groove and the negatively charged DNA and therefore destabilize basal strand separation...

... with Cas9 guided by different promoters. The challenge in building genetic circuits is getting enough...shape of the response function or how the output changes as a function of input. Usually that function is...enough genetic parts for complexity. We have characterized almost 600 terminators. That gives us enough... we’ve really been making tons of parts and characterizing the software to help you put it together. The...

...of the theme of my interview stories as the characteristic which the story demonstrates I possess.   Bullet...to present about your prior research. This is a chance to highlight your prior accomplishments and demonstrate...carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably still...their hand to ask a question or type it into the chat. Either way, just let the interviewers know up front...

...light-activated ion channels. Similarly, chemogenetic tools are designed channels that are chemically ...introduced in neurons and depending on the choice of channel protein, they can trigger neuronal excitation or...adeno-associated virus. Curr Protoc Microbiol. (2013); Chapter 14:Unit14D.5. PubMed PMID: 23686825. PubMed Central...

...Indicate the amino acid change as well as any functional consequences of that change. For example, “Mutated... Answer “What does this plasmid do?” in <200 characters. A descriptive purpose focuses on the practical...Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment...

...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...cell types and for viewing how gene expression changes through time or after genetic manipulation. The...tissue sample, several washes, and temperature changes to visualize the expression of specific genes. ...genes of interest and was first discovered and characterized in an unrelated worm (C. elegans) [2]. Delivering...

...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves... following transfection. In other words, when exchanging the medium after transfecting cells, replace ...electrostatic forces between these two negatively-charged membranes (Davis et al., 2002). Polybrene is one...306. PubMed PMID: 10634178. 8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young. "Recombination ...

...the only right way, and Indecisives, who keep changing their minds and have trouble making decisions.... of your supervisor’s management style, bring challenges to his or her attention as soon as you can. It... action items. This will give your supervisor a chance to review what you have discussed and add suggestions...mutually beneficial solutions. If you package your challenges with your supervisor into learning opportunities...

...mGold2t (Lee & Lai, et al., 2025). The team characterized and deposited a variety of constructs for visualizing...perform neuronal tracing, anterograde tracing poses challenges, from non-selective anterograde transport to ...transsynaptic label based on antibody-like sensors) changes that. Developed by the Arnold Lab, ATLAS is based...or delivered separately). Figure 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag...

...titration by qPCR.   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection...the density gradient material by dialysis/buffer exchange and further concentrate the virions if needed....Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J., Blouin-Tavel, V., Bosch, F., Bose, M., ...., Francis, J. D., … Snyder, R. O. (2010). Characterization of a recombinant adeno-associated virus type...

... 37 °C and 5% CO2 and get agitated with sudden changes in temperature (especially if you have air conditioning... to recover after they are passaged. This could change their growth rates and morphology, rendering their...numbers Keep an eye on the passage number. Cells change their growth rate and morphology at high passage...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

...AAV-containing 40% fraction is collected and buffer exchanged to remove the iodixanol and concentrate the purified...or the virus can be stored at 4 °C and buffer exchanged the next day. Refer to the AAV Iodixanol Gradient...correctly. When aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots...vivo use. Current protocols in molecular biology, Chapter 16, Unit 16.25. PubMed PMID: 18265393. Additional...

...genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., 2011). In fact, the... software. References and resources References Chadeuf G, Ciron C, Moullier P, Salvetti A (2005) Evidence...Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations....2015.32 Wright JF, Zelenaia O (2011) Vector Characterization Methods for Quality Control Testing of Recombinant...

...through wild populations. Whereas most genomic changes impose a fitness cost and are eliminated by natural...previous gene drive and thereby undo the genetic changes driven by the earlier intervention2. While an initial.... PubMed. Gantz, V. M. & Bier, E. The mutagenic chain reaction: A method for converting heterozygous to...10.1126/science.aaa5945. PubMed. DiCarlo, J. E., Chavez, A., Dietz, S. L., Esvelt, K. M. & Church, G. M...

...purify proteins can face any number of technical challenges depending on their protein of interest. After...After several decades of trying to address these challenges, researchers have amassed a considerable molecular...for your protein. If you’re working with an uncharacterized protein, or a protein for which a good antibody...KETAAAKFERQHMDS  1.8 Solubility and Affinity Abundance of charged and polar residues improves solubility; good for...

...General Meeting seven other times I had subsequently changed fields and my cohort of friends and colleagues ...evolutionary lineages share genes that can improve chances of survival for an individual, and eventually, ... cells regulate gene expression in response to changes in cell density. At the mixer, they meet other ...Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson, Tiffany Lowe-Power, Eric Griffis...

...has no doubt changed drastically over the past few decades. But no matter how much it changes, it will still... in one cell type but not in others. The same mechanism that allows enhancers to regulate gene expression....2021.108754 Panigrahi, A., & O'Malley, B. W. (2021). Mechanisms of enhancer action: the known and the unknown...